Now showing items 121-130 of 160
Modification of the 5′ Terminus of Oligodeoxyribonucleotides for Conjugation with Ligands
(Wiley Periodicals, Inc.IRL Press, 2001-07)
Ligands can be introduced at the 5′ terminus of an oligonucleotide by adding a linker to the ligand and modifying the 5′ terminus of the oligonucleotide. These are then reacted to give the ligand‐oligonucleotide conjugate. ...
In Vitro Selection of RNA Aptamers to a Protein Target by Filter Immobilization
(Oxford University PressWiley Periodicals, Inc., 2000-02)
This unit describes the selection of aptamers from a pool of single‐stranded RNA by binding to a protein target. Aptamers generated from this selection experiment can potentially function as protein inhibitors, and may ...
Molecular Modeling of Nucleic Acid Structure: Setup and Analysis
(Wiley Periodicals, Inc.Oxford University Press, 2001-09)
The last in a set of units by these authors, this unit addresses some important remaining questions about molecular modeling of nucleic acids. It describes how to choose an appropriate molecular mechanics force field; how ...
Thermal Methods for the Analysis of RNA Folding Pathways
(John Wiley & Sons, 2000-10)
Once a model of the secondary structure of an RNA has been deduced, thermal melting analysis can be used to determine whether the model accounts for all intramolecular interactions of the RNA, or whether noncanonical and ...
Molecular Modeling of Nucleic Acid Structure: Electrostatics and Solvation
(Wiley Periodicals, Inc.Oxford University Press, 2001-07)
This unit presents an overview of computer simulation techniques as applied to nucleic acid systems, ranging from simple in vacuo molecular modeling techniques to more complete all‐atom molecular dynamics treatments that ...
Denaturing Polyacrylamide Gel Electrophoresis
(Wiley Periodicals, Inc., 2000-02)
Thin polyacrylamide gels that contain a high concentration of urea as a denaturant are capable of resolving short (<500 nucleotides) single‐stranded fragments of DNA or RNA that differ in length by as little as one nucleotide. ...
Probing Nucleic Acid Structure with Shape‐Selective Rhodium and Ruthenium Complexes
(Wiley Periodicals, Inc.Humana Press, 2000-02)
In this unit, transition metal complexes are used as photochemical probes for the structure of RNA and DNA. The transition metal ion provides a rigid substitutionally inert framework and an octahedral geometry for ligand ...
Common Buffers and Stock Solutions
(CRC PressWiley Periodicals, Inc., 2000-02)
This appendix describes the preparation of buffers and reagents used in the manipulation of nucleic acids.RecipesAmmonium acetate, 10 MBCIP, 5% (w/v)DEPC (diethylpyrocarbonate)‐treated solutionsdNTPs: dATP, dTTP, dCTP, and ...
Methods to Crystallize RNA
(Wiley Periodicals, Inc.Academic Press, 2000-07)
Preparation of suitably large and wellâ ordered single crystals is usually the rateâ limiting step in the determination of the threeâ dimensional structure of RNAs and their complexes with proteins by Xâ ray crystallography. ...
Use of Electrophilic Substitution to Form Site‐Specific Cross‐Links in DNA
(Wiley Periodicals, Inc., 2001-09)
This unit describes the synthesis, purification, and analysis of modified oligodeoxynucleotides containing 4‐thio‐2′‐deoxyuridine, and the use of these modified oligodeoxynucleotides in conjunction with bis‐electrophilic ...