Now showing items 141-150 of 177
Examination of Aggregation Prone Proteins and their Higher Order Interactions by Ion Mobility-Mass Spectrometry.
(2015)
Amyloid forming peptides and proteins present an extreme challenge for modern analytical measurement techniques. When natively-folded biomolecules partition into misfolded forms, they produce a myriad of inherently unstable ...
Combining Active Site and Allosteric Inhibitors to Study Protein Tyrosine Kinases
(2016)
Kinases play a crucial role in a variety of cell signaling pathways that control processes such as: growth, motility, and angiogenesis. Not surprisingly, dysregulation of proper kinase signaling often leads to cancer. Since ...
Ultraquantitative Raman Spectral Cytometry to Measure the Cargo Capacity of Individual Macrophages
(2019)
This dissertation demonstrates the usefulness of Raman microscopy as a multipurpose, 'high content' cytometric analysis tool for characterizing the phenotype of diverse macrophage populations in terms of their molecular ...
Uncovering Hidden Dynamics in Living Systems Using Bayesian Statistics and Single-Molecule Microscopy
(2019)
Fluorescence microscopy is a powerful technique for understanding the organization, structure and dynamics of cells. Single-molecule imaging techniques extend our ability to probe cellular systems down to a range of only ...
Structure and Biochemistry of Cytochromes P450 Involved in the Biosynthesis of Macrolide Antibiotics
(2017)
Selective functionalization of chemically inert carbon-hydrogen (C–H) bonds embodies one of the grand challenges of organic chemistry and provides a key focus of research in the field. C–H functionalization can provide a ...
Interactions between the Translation Machinery and a Translational preQ1 Riboswitch.
(2015)
Gene expression is highly regulated with a diversity of regulation at the RNA level. In bacteria, regulation of mRNA translation into protein often occurs through RNA sequence features such as the Shine-Dalgarno (SD) ...
Synthesis and Biochemical Evaluation of Multifunctional Acetylcholinesterase Inhibitor Hybrids for Treatment of Alzheimer's Disease.
(2014)
Alzheimer’s disease (AD) places a significant and dramatically increasing burden on society. While pathological hallmarks of this neurodegenerative disorder are known, the exact cause remains unclear. Acetylcholinesterase ...
Design and Characterization of Bifunctional Glucocorticoid Ligands Capable of Producing Novel Transcriptional Profiles.
(2015)
The process of transcription underlies the expression of all gene profiles. Normal expression is caused by carefully coordinating the assembly of transcriptional proteins at specific genes, a procedure mediated through ...
Molecular Basis for Selective Late-Stage Transformations in Fungal Indole Alkaloid Biosynthesis
(2019)
The class of fungal indole alkaloids containing the bicyclo[2.2.2]diazaoctane ring is comprised of diverse molecules that display a range of biological activities. While much interest has been garnered due to their therapeutic ...
Structural Studies and Pharmacological Targeting of Protein Kinases in Obesity and Heart Disease
(2018)
Adrenergic receptors (AR) are G protein-coupled receptors (GPCRs) responsible for regulating physiological processes including the fight-or-flight response, muscle contraction, blood flow, and energy release. Numerous ...