Now showing items 161-170 of 177
Understanding Pre-mRNA Dynamics in Single Spliceosome Complexes.
(2013)
The spliceosome is a large RNA-protein complex that catalyzes pre-mRNA splicing by removing sequences called introns and joining of the remaining sequences, called exons, to produce a mature mRNA. Despite over a quarter ...
Interactions Between Fluorescent Molecules and Plasmonic Nanoparticles: A Super-Resolution Study
(2017)
Single-molecule super-resolution imaging techniques play an increasingly important role in studying complex fine structures, dynamics and interactions in nanoscale confined spaces. The resolution of this technique can be ...
Mass Spectrometry Based Techniques for In Vivo Neurochemical Monitoring.
(2012)
Neurotransmitter concentration in vivo reflects various behavioral and pharmacological events. Therefore, the measurement of neurochemical dynamics in live behaving animals’ brain is of crucial importance for the fundamental ...
Molecular Recognition of Inhibitors, Metal Ions and Substrates by Ribonuclease P.
(2013)
Ribonuclease P (RNase P) is a divalent metal ion-dependent endonuclease that catalyzes cleavage of the 5’ leader of precursor transfer RNA (pre-tRNA), an essential tRNA processing step in all domains of life. Bacterial ...
Investigation of the Formation of Z-DNA and Other Non-Canonical DNA Conformations Using a Combined Spectroscopic and Biochemical Approach
(2012)
Genomic sequences of DNA not only code for proteins, but also unique structural conformations encompassing a wide variety of helical and base-pairing geometries. Formation of these sequence-specific conformations is driven ...
Incorporation of Protein Flexibility in Structure-Based Drug Discovery of HIV-1 Protease and Bacterial Hsp70 Chaperone.
(2013)
Damm et al. utilized a multiple protein structure (MPS)-based method and discovery an allosteric site of HIV-1 protease (HIV-1p) that had not been described before. Based on this new site, a MPS-based pharmacophore model ...
Chemical Modulation of Phospho-Signaling Pathways Involved in Cancer.
(2015)
Advancements in our understanding of the molecular causes of cancer have led to the therapeutic targeting of key enzymes involved in cell growth. Kinase inhibitors have been the most successful forms of such targeted therapy ...
Mechanistic and Structural Insights into the Chemical Modulation of Amyloid Aggregation
(2016)
The aggregation of amyloid-beta (Abeta) is implicated in the etiology of Alzheimer’s disease (AD). Aggregation in vivo permits interactions with transition metal ions and lipids of the cell membrane. Abeta-metal interactions ...
Molecular Interactions between Various Model Cell Membranes and Membrane Active Peptides Studied by Sum Frequency Generation Vibrational Spectroscopy.
(2014)
Since the use of native cell membranes for biophysical studies is difficult, membrane mimetics are often used. However, if the model membrane is too simple, it may not be able to provide physiologically meaningful information. ...
Super-resolution Imaging of DNA Replisome Architecture and Dynamics in Live Bacillus Subtilis
(2018)
DNA replication happens in all living organisms and assures that the genome is accurately copied and maintained. The replisome is the molecular machine in cells that replicates DNA, and this protein assembly includes DNA ...