Now showing items 21-30 of 70
Coomassie staining provides routine (sub)femtomole in‐gel detection of intact proteoforms: Expanding opportunities for genuine Top‐down Proteomics
(The Royal Society of ChemistryWiley Periodicals, Inc., 2017-12)
Modified colloidal Coomassie Brilliant Blue (cCBB) staining utilising a novel destain protocol and near‐infrared fluorescence detection (nIRFD) rivals the in‐gel protein detection sensitivity (DS) of SYPRO Ruby. However, ...
Abacus: A computational tool for extracting and pre-processing spectral count data for label-free quantitative proteomic analysis
(WILEY-VCH Verlag, 2011-04)
We describe Abacus, a computational tool for extracting spectral counts from MS/MS data sets. The program aggregates data from multiple experiments, adjusts spectral counts to accurately account for peptides shared across ...
Perfluoro‐ tert ‐butyl‐homoserine as a sensitive 19 F NMR reporter for peptide–membrane interactions in solution
(Wiley Periodicals, Inc., 2013-05)
Conformation dependence of DNA electrophoretic mobility in a converging channel
(WILEY-VCH Verlag, 2010-08)
The electrophoresis of Λ-DNA is observed in a microscale converging channel where the center-of-masses trajectories of DNA molecules are tracked to measure instantaneous electrophoretic (EP) mobilities of DNA molecules of ...
Effect of cellular polyanion mimetics on tau peptide aggregation
(Wiley Periodicals, Inc., 2018-11)
On the Need to Develop Guidelines for Characterizing and Reporting Intrinsic Disorder in Proteins
(BIPM, SevresWiley Periodicals, Inc., 2019-03)
Since the early 2000s, numerous computational tools have been created and used to predict intrinsic disorder in proteins. At present, the output from these algorithms is difficult to interpret in the absence of standards ...
Label-free quantitative proteomics and SAINT analysis enable interactome mapping for the human Ser/Thr protein phosphatase 5
(WILEY-VCH Verlag, 2011-04)
Affinity purification coupled to mass spectrometry (AP-MS) represents a powerful and proven approach for the analysis of protein–protein interactions. However, the detection of true interactions for proteins that are ...
Dose‐dependent proteomic analysis of glioblastoma cancer stem cells upon treatment with γ‐secretase inhibitor
(WILEY‐VCH Verlag, 2011-12)
Notch signaling has been demonstrated to have a central role in glioblastoma (GBM) cancer stem cells (CSCs) and we have demonstrated recently that Notch pathway blockade by γ‐secretase inhibitor (GSI) depletes GBM CSCs and ...
How microbial proteomics got started
(WILEY‐VCH Verlag, 2011-08)
Publication in 1975 by Patrick O'Farrell of a procedure to separate the proteins of Escherichia coli in a two‐dimensional array on polyacrylamide gels marked the birth of the field now called proteomics. Although O'Farrell's ...
Development of selected reaction monitoring-MS methodology to measure peptide biomarkers in prostate cancer
(WILEY-VCH Verlag, 2010-10)
Prostate cancer is a leading cause of cancer-related death. The current modality of diagnosis, the measurement of serum PSA, not only suffers from lack of specificity, but does not distinguish clinical cases in which current ...