Now showing items 61-70 of 179
tRNA-guanine transglycosylase from Escherichia coli: Recognition of dimeric, unmodified tRNATyr
(Elsevier, 1994)
In order to probe the interaction between tRNA and the tRNA hypermodifying enzyme, tRNA-guanine transglycosylase (TGT) from Escherichia coli, we have undertaken the generation of E coli tRNATyr and analogues. During efforts ...
Corticotropin releasing hormone is involved in exercise-induced elevation in core temperature
(Elsevier, 1994)
To determine the involvement of corticotropin releasing factor (CRF) in exercise-induced elevation in core temperature of female rats, CRF antibody or vehicle was injected intracerebroventricularly (ICV) into rats that had ...
Whole skeletal muscle transplantation: Mechanisms responsible for functional deficits
(Wiley Subscription Services, Inc., A Wiley Company, 1994-04-05)
One aspect of tissue engineering of skeletal muscle involves the transposition and transplantation of whole muscles to treat muscles damaged by injury or disease. The transposition of whole muscles has been used for many ...
Nonsense mutations at Arg-1947 in two cases of familial neurofibromatosis type 1 in Japanese
(Springer-Verlag, 1994-01)
We report two familial cases of NF1 presenting as C to T transitions changing an Arg-1947 codon to a stop codon. In one of the two families, cosegregation of the mutation with NF1 was demonstrated, indicating this mutation ...
Relationship between cytotoxicity and conversion of thiosangivamycin analogs to toyocamycin analogs in cell culture medium
(Elsevier, 1994-08-17)
Non-nucleoside analogs of the pyrrolopyrimidine nucleosides toyocamycin, sangivamycin and thiosangivamycin have been synthesized and their cytotoxicity in mammalian cells determined. While studying the effects of ...
Expression of Corticotropin-Releasing Hormone Transgenes in Neurons of Adult and Developing Mice
(Elsevier, 1994-12)
The DNA sequences important for cell-specific expression and developmental regulation of corticotropin-releasing hormone (CRH) were analyzed in transgenic mice. A construct containing 0.5 kb of CRH 5' flanking DNA linked ...
Development of a sensitive, highly controlled assay for molecular detection of the Philadelphia chromosome in patients with chronic myelogenous leukemia
(Elsevier, 1994)
The Philadelphia chromosome (Ph1), present in [ges]95% of chronic myelogenous leukemia (CML) patients, is a well-characterized translocation that results in a unique chimeric gene product (BCR/ABL) with transforming ...
Application of the gel shift assay to study the affinity and specificity of anti-DNA autoantibodies
(Elsevier, 1994-12-28)
We have demonstrated that the gel shift assay, a powerful method to study protein [middle dot] DNA interactions under equilibrium conditions, is both an accurate and precise method to measure the affinity of anti-DNA [middle ...
Tyrosine kinase activity associated with the CD7 antigen: correlation with regulation of T cell integrin function
(WILEY-VCH Verlag GmbH, 1994-11)
Rapid up-regulation of the functional activity of integrin adhesion receptors is a hallmark of T cell activation. Monoclonal antibody engagement of the CD7 antigen on human T cells results in an increase in Β1 and Β2 ...