Now showing items 891-897 of 897
Thiol-Mediated Redox Modulation of the Adaptive Immune Response.
(2010)
T cell activation and proliferation requires a reducing microenvironment that is provided by antigen presenting cells especially dendritic cells (DCs). Naturally occurring CD4+CD25+Foxp3+ regulatory T cells (Tregs) suppress ...
Regulating Gene Expression Through DNA Mechanics: Tightly Looped DNA Represses Transcription.
(2010)
It is now widely accepted that the mechanical state of DNA can play a major role in regulating the activity of RNA polymerase (RNAP). Not only have the global levels of supercoiling been shown to regulate transcription, ...
The Landscape of C. elegans 3'UTRs.
(2011)
The 3’ untranslated regions (3’UTRs) of mRNAs have recently been shown to play major roles in gene regulation by interaction with small (21-26nt length) RNAs, such as microRNAs and small interfering RNAs (siRNAs). Recent ...
Engineering the Quantitative PCR Assay for Decreased Cost and Complexity.
(2011)
The quantitative polymerase chain reaction (qPCR) is an assay of target nucleic acid concentration. Clinical applications of quantitative PCR include measurement of HIV viral load, measurement of bacterial infection, and ...
Optimization of RiPCA for the Live-Cell Detection of Pre-MicroRNA-Protein Interactions
(Wiley Periodicals, Inc., 2022-12-16)
Advancements in methods for identifying RNA-protein interactions (RPIs) on a large scale has necessitated the development of assays for validation of these interactions, particularly in living cells. We previously reported ...
Transparent and Self-Healing Elastomers for Reconfigurable 3D Materials
(Wiley Periodicals, Inc., 2022-12)
Transparent soft materials are widely used in applications ranging from packaging to flexible displays, wearable devices, and optical lenses. Nevertheless, soft materials are susceptible to mechanical damage, leading to ...
BayeStab: Predicting effects of mutations on protein stability with uncertainty quantification
(John Wiley & Sons, Inc., 2022-11)
Predicting protein thermostability change upon mutation is crucial for understanding diseases and designing therapeutics. However, accurately estimating Gibbs free energy change of the protein remained a challenge. Some ...