Now showing items 1631-1640 of 1640
Development of Modular, Droplet Microfluidic Workflows to Investigate Cellular Heterogeneity
(2023)
Cells exist in unique contexts within a population, but miniscule differences in their environment, epigenome, and cell cycle produce an assortment of observable characteristics in cells. Conventional cellular analysis ...
Innovations in Droplet Microfluidics for Enzyme Evolution
(2020)
High throughput screening (HTS) is a critical technology in discovery and process chemistry. Typically, HTS has relied on robotic handling of well plates of samples to rapidly perform and assess reactions, but novel ...
Directed Evolution of Stabilized Peptides with Bacterial Display
(2020)
Interactions between proteins govern cellular and the body’s states, including aberrant interactions found in diseases such as in cancers and infections. Small molecule drugs are not ideal in targeting these interactions ...
PSM Small Peptide Toxins Form Functional Bacterial Amyloids in the Staphylococcus aureus Biofilm Matrix.
(2014)
Staphylococcus aureus is a Gram-positive opportunistic pathogen colonizing mammalian hosts. Like many bacteria, S. aureus forms multicellular aggregates called biofilms. Biofilms are surface-associated communities of ...
Hsp70 chaperone Proteins and their Interactions with Various Drugs as Studied by Nuclear Magnetic Resonance.
(2011)
Chaperone proteins and their cochaperones are perhaps one of the most intriguing systems for investigation. Ubiquitous in nature, they can be found in every organism and that perhaps is the reason that their sequence shows ...
Molecular Dynamics Simulation of Phase and Structural Transitions in Model Lung Surfactant Mixtures.
(2010)
Lung surfactant (LS) is a complex mixture of lipids and proteins that reduces and regulates the surface tension in the lungs, thereby decreasing the work of breathing. A thorough understanding of LS function is critical ...
Engineering the Quantitative PCR Assay for Decreased Cost and Complexity.
(2011)
The quantitative polymerase chain reaction (qPCR) is an assay of target nucleic acid concentration. Clinical applications of quantitative PCR include measurement of HIV viral load, measurement of bacterial infection, and ...
Transparent and Self-Healing Elastomers for Reconfigurable 3D Materials
(Wiley Periodicals, Inc., 2022-12)
Transparent soft materials are widely used in applications ranging from packaging to flexible displays, wearable devices, and optical lenses. Nevertheless, soft materials are susceptible to mechanical damage, leading to ...
Identification of regulatory variants of carboxylesterase 1 (CES1): A proof-of-concept study for the application of the Allele-Specific Protein Expression (ASPE) assay in identifying cis-acting regulatory genetic polymorphisms
(Wiley Periodicals, Inc., 2023-01)
It is challenging to study regulatory genetic variants as gene expression is affected by both genetic polymorphisms and non-genetic regulators. The mRNA allele-specific expression (ASE) assay has been increasingly used for ...
Super-Strong Hydrogel Composites Reinforced with PBO Nanofibers for Cartilage Replacement
(Wiley Periodicals, Inc.Imperial College Press, 2023-02)
Cartilage replacement materials exhibiting a set of demanding properties such as high water content, high mechanical stiffness, low friction, and excellent biocompatibility are quite difficult to achieve. Here, ...