Now showing items 131-140 of 165
Probing Nucleic Acid Structure with Shape‐Selective Rhodium and Ruthenium Complexes
(Wiley Periodicals, Inc.Humana Press, 2000-02)
In this unit, transition metal complexes are used as photochemical probes for the structure of RNA and DNA. The transition metal ion provides a rigid substitutionally inert framework and an octahedral geometry for ligand ...
Common Buffers and Stock Solutions
(CRC PressWiley Periodicals, Inc., 2000-02)
This appendix describes the preparation of buffers and reagents used in the manipulation of nucleic acids.RecipesAmmonium acetate, 10 MBCIP, 5% (w/v)DEPC (diethylpyrocarbonate)‐treated solutionsdNTPs: dATP, dTTP, dCTP, and ...
Methods to Crystallize RNA
(Wiley Periodicals, Inc.Academic Press, 2000-07)
Preparation of suitably large and wellâ ordered single crystals is usually the rateâ limiting step in the determination of the threeâ dimensional structure of RNAs and their complexes with proteins by Xâ ray crystallography. ...
Use of Electrophilic Substitution to Form Site‐Specific Cross‐Links in DNA
(Wiley Periodicals, Inc., 2001-09)
This unit describes the synthesis, purification, and analysis of modified oligodeoxynucleotides containing 4‐thio‐2′‐deoxyuridine, and the use of these modified oligodeoxynucleotides in conjunction with bis‐electrophilic ...
Molecular Modeling of Nucleic Acid Structure: Energy and Sampling
(Wiley Periodicals, Inc.Oxford University Press, 2001-04)
An overview of computer simulation techniques as applied to nucleic acid systems is presented. This unit expands an accompanying overview unit (UNIT ) by discussing methods used to treat the energy and sample representative ...
Synthesis of Oligoribonucleotides Containing N6‐Alkyladenosine and 2‐Methylthio‐N6‐Alkyladenosine
(American Society for Microbiology PressWiley Periodicals, Inc., 2004-06)
The N6‐alkyladenosines and 2‐methylthio‐N6‐alkyladenosines are the most common modified adenosine nucleosides, and transfer ribonucleic acids (tRNA) are particularly rich in these modified nucleosides. They are present at ...
Probing RNA Structure and Function by Nucleotide Analog Interference Mapping
(Wiley Periodicals, Inc., 2004-06)
Nucleotide analog interference mapping (NAIM) can be used to simultaneously, yet individually, identify structurally or catalytically important functional groups within an RNA molecule. Phosphorothioate‐tagged nucleotides ...
Calorimetry of Nucleic Acids
(John Wiley & Sons, 2000-02)
This unit describes the application of differential scanning and isothermal titration calorimetry (DSC and ITC) to the study of the thermodynamics of nucleic acid structure. DSC is used to study order‐disorder transitions. ...
Synthesis of Protected 2′‐Deoxy‐2′‐fluoro‐β‐D‐arabinonucleosides
(Wiley Periodicals, Inc.International Society for Nucleosides, Nucleotides, and Nucleic Acids, 2002-09)
This unit describes in detail the preparation of protected 2’‐deoxy‐2’‐fluoroarabinonucleosides. These building blocks are required for the synthesis of 2’‐deoxy‐2’‐fluoroarabinonucleic acid (2’F‐ANA), an oligonucleotide ...
Probing RNA Folding Pathways by RNA Fingerprinting
(John Wiley & Sons, 2000-10)
This unit provides protocols for using native polyacrylamide gel electrophoresis to distinguish folding and unfolding conformers of RNA. It is useful for studying conformers that can exchange in a period of minutes or ...