Now showing items 61-70 of 578
Development of Channeled Nanofibrous Scaffolds for Oriented Tissue Engineering
(WILEY‐VCH Verlag, 2012-06)
A tissue‐engineering scaffold resembling the structure of the natural extracellular matrix can often facilitate tissue regeneration. Nerve and tendon are oriented micro‐scale tissue bundles. In this study, a method combining ...
Reexamination of the Direct Electrochemical Reduction of S ‐Nitrosothiols
(WILEY‐VCH Verlag, 2013-04)
We report here on the electrochemical reduction of S ‐nitrosothiol species (RSNO). Nitric oxide (NO) is the reported common product from electrochemically reduced RSNOs at physiological pH. However, studies here at pH 7.4 ...
Protection of 5′‐Hydroxy Functions of Nucleosides
(IRL PressWiley Periodicals, Inc., 2000-02)
The 5‐hydroxy group is the primary hydroxy group of nucleosides. It is mandatory to protect 5‐hydroxyls in all methods of oligonucleotide synthesis that require nucleoside synthons. This unit discusses a wide variety of ...
Proteomic analysis of cold adaptation in a Siberian permafrost bacterium – Exiguobacterium sibiricum 255–15 by two-dimensional liquid separation coupled with mass spectrometry
(WILEY-VCH Verlag, 2006-10)
Bacterial cold adaptation in Exiguobacterium sibiricum 255–15 was studied on a proteomic scale using a 2-D liquid phase separation coupled with MS technology. Whole-cell lysates of E. sibiricum 255–15 grown at 4°C and ...
Bio‐Orthogonal Polymer Coatings for Co‐Presentation of Biomolecules
(WILEY‐VCH Verlag, 2012-04-23)
Controlled presentation of biomolecules on synthetic substrates is an important aspect for biomaterials development. If the immobilization of multiple biomolecules is required, highly efficient orthogonal surface chemistries ...
SRM targeted proteomics in search for biomarkers of HCV ‐induced progression of fibrosis to cirrhosis in HALT ‐ C patients
(Wiley Periodicals, Inc.Springer, 2012-04)
Characterization of Tertiary Folding of RNA by Circular Dichroism and Urea
(Wiley Periodicals, Inc., 2001-04)
CD spectroscopy can be used to monitor RNA tertiary folding transitions that may not be observable by absorbance spectroscopy. With the use of computer‐controlled titrators, data can be acquired rapidly, and accurate ...
Biophysical Analysis of Nucleic Acids
(VCH PublishersWiley Periodicals, Inc., 2000-02)
This overview unit provides a thorough overview of biophysical methods used for structure analysis, including X‐ray diffraction, nuclear magnetic resonance, optical spectroscopy, theoretical and computational methods, and ...
References to Commonly Used Techniques
(John Wiley & Sons, Inc, 2000-02)
Many of the protocols in this manual assume a knowledge of basic (chemical and biochemical) techniques. Although some of these are outside the scope of Current Protocols in Nucleic Acid Chemistry (CPNC), several basic ...
Biomechanics and Energetics of Bipedal Locomotion on Uneven Terrain.
(2015)
Humans navigate uneven terrain in their everyday lives. From trails, grass, and uneven sidewalks, we constantly adapt to various surfaces in our environment. Past research has shown that walking on natural terrain, compared ...