Now showing items 21-30 of 184
A novel phosphate-regulated expression vector in Escherichia coli
(Elsevier, 1990-05-31)
The ugp promoter (pugp) responsible for expression of the binding-protein-dependent sn-glycerol-3-phosphate transport system in Escherichia coli was cloned into a small multicopy plasmid pTER5, a derivative of pBR322, ...
mnd2: A New Mouse Model of Inherited Motor Neuron Disease
(Elsevier, 1993-06)
The autosomal recessive mutation mnd2 results in early onset motor neuron disease with rapidly progressive paralysis, severe muscle wasting, regression of thymus and spleen, and death before 40 days of age. mnd2 has been ...
TECHNICAL REPORT: Rapid confirmation of gene targeting in embryonic stem cells using two long-range PCR techniques
(Kluwer Academic Publishers; Chapman and Hall ; Springer Science+Business Media, 1998-03)
Gene targeting in mouse embryonic stem (ES) cells generally includes the analysis of numerous colonies to identify a few with mutations resulting from homologous recombination with a targeting vector. Thus, simple and ...
Localization of the gene encoding R[kappa]B (NFRKB), a tissue-specific DNA binding protein, to chromosome 11q24-q25
(Elsevier, 1992-10)
Although NF (nuclear factor)-[kappa]B binds in vitro to several of the [kappa]B regulatory elements found in cellular and viral genes, another DNA binding protein, R[kappa]B, also binds to a related variant of the [kappa]B ...
Linkage of the multiple endocrine neoplasia type 2B gene (MEN2B) to chromosome 10 markers linked to MEN2A
(Elsevier, 1990-10)
The syndrome of multiple endocrine neoplasia type 2B (MEN 2B) resembles that of MEN 2A in that both include medullary carcinoma of the thyroid, pheochromocytoma, and autosomal dominant inheritance, but is distinct in that ...
Localization of the panhypopituitary dwarf mutation (df) on mouse chromosome 11 in an intersubspecific backross
(Elsevier, 1991-07)
Ames dwarf (df) is an autosomal recessive mutation characterized by severe dwarfism and infertility. This mutation provides a mouse model for panhypopituitarism. The dwarf phenotype results from failure in the differentiation ...
Development of Molecular Genetic Interventions for HIV Infection
(John Wiley & Sons, 1997-02)
This unit includes a set of protocols for the ex vivo transfer of genes into CD4+ T cells, to be used in the initial evaluation of genes protecting against HIV infection in gene therapy protocols. The describes isolating ...
The gene for a novel epidermal antigen maps near the neurofibromatosis 1 gene
(Elsevier, 1992-10)
Recently the M17S1 gene, encoding an epidermal antigen thought to play a role in cell adhesion, was mapped to chromosome bands 17q11-q12, placing it in the vicinity of the gene for the genetic disorder neurofi-bromatosis ...
A microsatellite polymorphism associated with the PLC1 (phospholipase C) locus: Identification, mapping, and linkage to the MODY locus on chromosome 20
(Elsevier, 1992-07)
A highly polymorphic (dC-dA)n[middle dot](dG-dT)n dinucleotide repeat at the PLC1 locus on human chromosome 20 has been identified. Primers flanking the dinucleotide repeat were used for PCR amplification of the repeat ...