Now showing items 31-40 of 392
Solubilization of hen brain neurotoxic esterase in dimethylsulfoxide
(Elsevier, 1985-10-15)
Neurotoxic esterase is the putative site of initiation of organophosphorus-induced neuropathy. The protein is membrane-associated and will thus require solubilization before it can be purified. Its enzymic activity was ...
Michael addition of thiols with 4-methyleneglutamic acid: Preparation of adducts, their properties and presence in peanuts
(Elsevier, 1982-04-29)
The thioethers, S-(4-amino-2,4-dicarboxybutyl)cysteamine, S-(4-amino-2,4-dicarboxybutyl)cysteine and S-(4-amino-2,4-dicarboxybutyl)glutathione, were synthesized by a Michael addition between 4-methyleneglutamic acid and ...
Characterization of a topoisomerase-like activity at specific hypersensitive sites in the Drosophila histone gene cluster
(Elsevier, 1989-07-14)
It is well known that treatment of DNA-topoisomerase complexes with SDS induces cleavage of the DNA by trapping a reactive intermediate in which the topoisomerase is covalently linked to the terminal phosphates of the cut ...
Teratogenic action of platinum thymine blue
(Elsevier, 1982-08-23)
The teratogenic activity of the antitumor agent cisplatinum-2-thymine (platinum thymine blue) was investigated in rats. Pregnant Wistar-derived albino rats were given single ip injections of an aqueous solution of platinum ...
Optimal activity times and habitat choice of moose
(Springer-Verlag, 1981-02)
A set of concepts was presented which could be used to model an animal's activity cycle and habitat choice as an optimization process. The model was applied to moose ( Alces alces ) summer activity and its predictions were ...
Isolation of an acid protease from rabbit reticulocytes and evidence for its role in processing redox proteins during erythroid maturation
(Elsevier, 1981-06-30)
A protease which generates a soluble hemepeptide from bovine liver microsomal cytochrome has been isolated from the membrane fraction of rabbit reticulocytes. Inhibition by pepstatin and an acidic pH optimum indicate that ...
Monovalent cations (Na+, K+, Cs+) inhibit calcium activation of photosynthetic oxygen evolution
(Elsevier, 1989-02-13)
In the absence of the 17 and 23 kDa water-soluble polypeptides, the oxygen-evolving complex of photosystem II requires addition of Cl- and Ca2+ to maximize rates of oxygen evolution activity. We have found that monovalent ...
Differing calcium requirements for regulatory effects of ATP, ATP[gamma]S and adenosine on O2 responses of human neutrophils
(Elsevier, 1988-07-29)
In formyl peptide stimulated human neutrophils the enhancement of O2 responses by ATP and ATP[gamma]S requires extracellular calcium. In contrast, the inhibitory effects of adenosine are independent of a calcium requirement. ...
Herpes simplex virus thymidine kinase gene is stably maintained and expressed in cells transformed by protoplast fusion
(Kluwer Academic Publishers-Plenum Publishers; Plenum Publishing Corporation ; Springer Science+Business Media, 1984-03)
We examined a series of transformed cell lines resulting from transfer of the herpes simplex virus type 1 thymidine kinase gene to Ltk − cells by protoplast fusion gene transfer. We show that multiple copies of the ...
Isolation of chromosome clusters from metaphase-arrested HeLa cells
(Springer-Verlag, 1982-08)
We have developed a simplified approach for the isolation of metaphase chromosomes from HeLa cells. In this method, all the chromosomes from a cell remain together in a bundle which we call a “metaphase chromosome cluster”. ...