Flow‐aligned, single‐shot fiber diffraction using a femtosecond X‐ray free‐electron laser
Popp, David; Loh, N. Duane; Zorgati, Habiba; Ghoshdastider, Umesh; Liow, Lu Ting; Ivanova, Magdalena I.; Larsson, Mårten; DePonte, Daniel P.; Bean, Richard; Beyerlein, Kenneth R.; Gati, Cornelius; Oberthuer, Dominik; Arnlund, David; Brändén, Gisela; Berntsen, Peter; Cascio, Duilio; Chavas, Leonard M. G.; Chen, Joe P. J.; Ding, Ke; Fleckenstein, Holger; Gumprecht, Lars; Harimoorthy, Rajiv; Mossou, Estelle; Sawaya, Michael R.; Brewster, Aaron S.; Hattne, Johan; Sauter, Nicholas K.; Seibert, Marvin; Seuring, Carolin; Stellato, Francesco; Tilp, Thomas; Eisenberg, David S.; Messerschmidt, Marc; Williams, Garth J.; Koglin, Jason E.; Makowski, Lee; Millane, Rick P.; Forsyth, Trevor; Boutet, Sébastien; White, Thomas A.; Barty, Anton; Chapman, Henry; Chen, Swaine L.; Liang, Mengning; Neutze, Richard; Robinson, Robert C.
2017-12
Citation
Popp, David; Loh, N. Duane; Zorgati, Habiba; Ghoshdastider, Umesh; Liow, Lu Ting; Ivanova, Magdalena I.; Larsson, Mårten ; DePonte, Daniel P.; Bean, Richard; Beyerlein, Kenneth R.; Gati, Cornelius; Oberthuer, Dominik; Arnlund, David; Brändén, Gisela ; Berntsen, Peter; Cascio, Duilio; Chavas, Leonard M. G.; Chen, Joe P. J.; Ding, Ke; Fleckenstein, Holger; Gumprecht, Lars; Harimoorthy, Rajiv; Mossou, Estelle; Sawaya, Michael R.; Brewster, Aaron S.; Hattne, Johan; Sauter, Nicholas K.; Seibert, Marvin; Seuring, Carolin; Stellato, Francesco; Tilp, Thomas; Eisenberg, David S.; Messerschmidt, Marc; Williams, Garth J.; Koglin, Jason E.; Makowski, Lee; Millane, Rick P.; Forsyth, Trevor; Boutet, Sébastien ; White, Thomas A.; Barty, Anton; Chapman, Henry; Chen, Swaine L.; Liang, Mengning; Neutze, Richard; Robinson, Robert C. (2017). "Flow‐aligned, single‐shot fiber diffraction using a femtosecond X‐ray free‐electron laser." Cytoskeleton 74(12): 472-481.
Abstract
A major goal for X‐ray free‐electron laser (XFEL) based science is to elucidate structures of biological molecules without the need for crystals. Filament systems may provide some of the first single macromolecular structures elucidated by XFEL radiation, since they contain one‐dimensional translational symmetry and thereby occupy the diffraction intensity region between the extremes of crystals and single molecules. Here, we demonstrate flow alignment of as few as 100 filaments (Escherichia coli pili, F‐actin, and amyloid fibrils), which when intersected by femtosecond X‐ray pulses result in diffraction patterns similar to those obtained from classical fiber diffraction studies. We also determine that F‐actin can be flow‐aligned to a disorientation of approximately 5 degrees. Using this XFEL‐based technique, we determine that gelsolin amyloids are comprised of stacked β‐strands running perpendicular to the filament axis, and that a range of order from fibrillar to crystalline is discernable for individual α‐synuclein amyloids.Publisher
Academic Press Wiley Periodicals, Inc.
ISSN
1949-3584 1949-3592
Other DOIs
Types
Article
Metadata
Show full item recordCollections
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.