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Detection of CTC Clusters and a Dedifferentiated RNAâ Expression Survival Signature in Prostate Cancer
dc.contributor.authorKozminsky, Molly
dc.contributor.authorFouladdel, Shamileh
dc.contributor.authorChung, Jae‐seung
dc.contributor.authorWang, Yugang
dc.contributor.authorSmith, David C.
dc.contributor.authorAlva, Ajjai
dc.contributor.authorAzizi, Ebrahim
dc.contributor.authorMorgan, Todd
dc.contributor.authorNagrath, Sunitha
dc.date.accessioned2019-02-12T20:23:28Z
dc.date.available2020-03-03T21:29:36Zen
dc.date.issued2019-01
dc.identifier.citationKozminsky, Molly; Fouladdel, Shamileh; Chung, Jae‐seung ; Wang, Yugang; Smith, David C.; Alva, Ajjai; Azizi, Ebrahim; Morgan, Todd; Nagrath, Sunitha (2019). "Detection of CTC Clusters and a Dedifferentiated RNAâ Expression Survival Signature in Prostate Cancer." Advanced Science 6(2): n/a-n/a.
dc.identifier.issn2198-3844
dc.identifier.issn2198-3844
dc.identifier.urihttps://hdl.handle.net/2027.42/147791
dc.description.abstractRates of progression and treatment response in advanced prostate cancer are highly variable, necessitating nonâ invasive methods to assess the molecular characteristics of these tumors in real time. The unique potential of circulating tumor cells (CTCs) to serve as a clinically useful liquid biomarker is due to their ability to inform via both enumeration and RNA expression. A microfluidic graphene oxideâ based device (GO Chip) is used to isolate CTCs and CTC clusters from the whole blood of 41 men with metastatic castrationâ resistant prostate cancer. Additionally, the expression of 96 genes of interest is determined by RTâ qPCR. Multivariate analyses are conducted to determine the genes most closely associated with overall survival, PSA progression, and radioclinical progression. A preliminary signature, comprising high expression of stemness genes and low expression of epithelial and mesenchymal genes, potentially implicates an undifferentiated CTC phenotype as a marker of poor prognosis in this setting.A microfluidic graphene oxideâ based device (GO Chip) is used to isolate circulating tumor cells (CTCs) and CTC clusters from the whole blood of 41 metastatic castrationâ resistant prostate cancer patients. A preliminary RNA signature, comprising high expression of stemness genes and low expression of epithelial and mesenchymal genes, potentially implicates an undifferentiated CTC phenotype as a marker of poor prognosis.
dc.publisherSpringer
dc.publisherWiley Periodicals, Inc.
dc.subject.othercirculating tumor cells
dc.subject.othermicrofluidics
dc.subject.otherprostate cancer
dc.subject.otherRNA expression
dc.subject.otherGO Chip
dc.titleDetection of CTC Clusters and a Dedifferentiated RNAâ Expression Survival Signature in Prostate Cancer
dc.typeArticleen_US
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelMaterials Science and Engineering
dc.subject.hlbtoplevelEngineering
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/147791/1/advs887.pdf
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/147791/2/advs887-sup-0001-S1.pdf
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/147791/3/advs887_am.pdf
dc.identifier.doi10.1002/advs.201801254
dc.identifier.sourceAdvanced Science
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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