Now showing items 31-40 of 1039
Analysis of neuropeptide gene expression by transfection of DNA into cell lines
(Kluwer Academic Publishers; Tissue Culture Association ; Springer Science+Business Media, 1993-06)
The transcriptional regulation of neuropeptide genes by cAMP is often directed by a cAMP responsive enhancer (CRE) upstream to the promoter of the genes. The identity of the CRE was determined by transient transfection ...
Synthesis of normal and variant human hypoxanthine-guanine phosphoribosyltransferase in Escherichia coli
(Elsevier, 1993-01-30)
Naturally occurring mutations in hypoxanthine-guanine phosphoribosyltransferase (HPRT) have been identified by amino acid sequencing, cDNA cloning, and direct nucleotide sequencing of PCR-amplified transcripts. To determine ...
Glutamic acid-insensitive [3H]kainic acid binding in goldfish brain
(Elsevier, 1992-01-31)
Kainic acid is supposed to be a specific agonist for a subclass of excitatory glutamate receptors in the vertebrate CNS. An investigation of (2 nM) [3H]kainic acid binding sites in goldfish brain, using quantitative ...
Nucleotide Sequence Analysis of 77.7 kb of the Human V[beta] T-Cell Receptor Gene Locus: Direct Primer-Walking Using Cosmid Template DNAs
(Elsevier, 1994-03-15)
The nucleotide sequence of 77.7 kb from the human T-cell receptor [beta]-chain locus was determined directly from three overlapping cosmid clones using the primer-walking approach. Computer-aided analyses of this sequence ...
Coupling of dihydroriboflavin oxidation to the formation of the higher valence states of hemeproteins
(Elsevier, 1991-11-27)
SummaryThe reactions between hydrogen peroxide and hemeproteins have been coupled to the oxidation of dihydroriboflavin so as to provide a simple method for measuring the rate constant of hemeprotein peroxidation. ...
A new 3D HN(CA)HA experiment for obtaining fingerprint H N -H α cross peaks in 15 N- and 13 C-labeled proteins
(Kluwer Academic Publishers; ESCOM Science Publishers B.V. ; Springer Science+Business Media, 1992-03)
A new 3D 1 H− 15 N− 13 C triple resonance experiment is presented that provides in-phase absorptive cross peaks between amide protons and α-protons of the same and the sequentially preceding residue. The experiment yields ...
Protease nexin-1 activity in cultured Schwann cells
(Elsevier, 1991-07-08)
We report that protease nexin-1 (PN-1), a serine protease inhibitor known to have neurite-promoting effects, is made by Schwann cells in tissue culture. Three modalities have been used to demonstrate the presence of PN-1 ...
Assessment of foveal cone photoreceptors in Stargardt's macular dystrophy using a small dot detection task
(Elsevier, 1993-07)
We measured frequency-of-seeing curves for tiny (1.125 and 3.375 min arc) stimuli flashed briefly at absolute threshold to estimate the density of foveal cones in normals and in subjects with Stargardt's macular dystrophy. ...
Post-translational processing of gastrin in neoplastic human colonic tissues
(Elsevier, 1992-12-15)
Gastrin has been postulated to stimulate proliferation in colorectal neoplasms. Although gastrin mRNA has been demonstrated to be present in colon cancer cell lines, the intact peptide had not been recovered from human ...