DNA polymerase in nuclei isolated from herpes simplex virus type-2-infected cells. Characterization of the reaction product and inhibition by substrate analogs
dc.contributor.author | Barnett, Jimmy W. | en_US |
dc.contributor.author | Reinke, C. Michael | en_US |
dc.contributor.author | Turk, Steven R. | en_US |
dc.contributor.author | Drach, John C. | en_US |
dc.date.accessioned | 2006-04-07T18:30:54Z | |
dc.date.available | 2006-04-07T18:30:54Z | |
dc.date.issued | 1984-02-24 | en_US |
dc.identifier.citation | Barnett, Jimmy W., Reinke, C. Michael, Turk, Steven R., Drach, John C. (1984/02/24)."DNA polymerase in nuclei isolated from herpes simplex virus type-2-infected cells. Characterization of the reaction product and inhibition by substrate analogs." Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression 781(1-2): 130-142. <http://hdl.handle.net/2027.42/24900> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T1V-47S5YFF-H9/2/1028d1c25c4c921d5dc98e29cb8dbbe1 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/24900 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6320890&dopt=citation | en_US |
dc.description.abstract | Nuclei isolated from herpes simplex virus (HSV) type 2-infected KB cells were examined for their capacity to serve as an in situ source of herpes DNA polymerase. In contrast to purified enzymes with added template, approx. 80% of the DNA synthesized in isolated nuclei was viral. The average size of DNA fragments labeled in vitro was 3.2[middle dot]106 Da. Based on an increase in DNA density when nuclei were incubated in the presence of BrdUTP rather than dTTP, 16% of the nucleotides were added during the in vitro reaction. Sucrose gradient analysis of DNA polymerase activity in extracts of isolated nuclei demonstrated the nearly exclusive presence of herpes DNA polymerase. Km concentrations for the four dNTPs were from 0.14 to 0.55 [mu]M. DNA synthesis was inhibited competitively by the 5'-triphosphates of ara-A and ara-C (Ki = 0.03 and 0.22 [mu]M, respectively) but not by the 5'-triphosphate of dideoxythymidine. aATP also served as a substrate (Km = 0.014 [mu]M) for the reaction. We conclude that nuclei from HSV-infected cells have significant advantages for the detailed study of inhibitors of herpesvirus replication. | en_US |
dc.format.extent | 994431 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | DNA polymerase in nuclei isolated from herpes simplex virus type-2-infected cells. Characterization of the reaction product and inhibition by substrate analogs | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Materials Science and Engineering | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | School of Dentistry, University of Michigan, Ann Arbor, MI 48109, U.S.A | en_US |
dc.contributor.affiliationum | School of Dentistry, University of Michigan, Ann Arbor, MI 48109, U.S.A | en_US |
dc.contributor.affiliationum | School of Dentistry, University of Michigan, Ann Arbor, MI 48109, U.S.A | en_US |
dc.contributor.affiliationum | School of Dentistry, University of Michigan, Ann Arbor, MI 48109, U.S.A | en_US |
dc.identifier.pmid | 6320890 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/24900/1/0000327.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0167-4781(84)90131-3 | en_US |
dc.identifier.source | Biochimica et Biophysica Acta | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.