Electrolysis-induced myocardial dysfunction : A novel method for the study of free radical mediated tissue injury
dc.contributor.author | Jackson, Charles V. | en_US |
dc.contributor.author | Mickelson, Judith K. | en_US |
dc.contributor.author | Stringer, Kathleen | en_US |
dc.contributor.author | Rao, Parinam S. | en_US |
dc.contributor.author | Lucchesi, Benedict Robert | en_US |
dc.date.accessioned | 2006-04-07T19:29:20Z | |
dc.date.available | 2006-04-07T19:29:20Z | |
dc.date.issued | 1986-07 | en_US |
dc.identifier.citation | Jackson, Charles V., Mickelson, Judith K., Stringer, Kathleen, Rao, Parinam S., Lucchesi, Benedict R. (1986/07)."Electrolysis-induced myocardial dysfunction : A novel method for the study of free radical mediated tissue injury." Journal of Pharmacological Methods 15(4): 305-320. <http://hdl.handle.net/2027.42/26123> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B73F9-477XNNP-MK/2/e91118d76faf2de4b11f020e6921e572 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/26123 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=3724201&dopt=citation | en_US |
dc.description.abstract | Oxygen-derived free radicals and other oxidizing species are thought to be involved in inflammation and ischemic tissue injuries. Recently, oxygen-derived free radicals also have been implicated in tissue injury of the myocardium subjected to ischemia/reperfusion. The purpose of this investigation was to determine if electrolysis of a physiological buffer would serve as a source of free radicals, and if these radicals would lead to alterations in myocardial function. Isolated Langendorff-perfused rabbit hearts perfused with buffer subjected to a 20 mA D.C. current for 2 min demonstrated significant increases in coronary perfusion pressure (37 +/- 6 mmHg), left ventricular end diastolic pressure (41 +/- 7 mmHg), and loss in left ventricular developed pressure (35 +/- 5%). The free radical scavengers, Superoxide dismutase and a combination of tryptophan plus glycine, were effective in protecting the hearts from the effects of electrolysis. The presence of free radicals was semiquantitated with a radical-luminol chemiluminescent assay. In this assay a variety of radical scavengers and antioxidants were effective (i.e., dimethyl sulfoxide, nitro blue tetrazolium, ascorbate, superoxide dismutase, 1, 3-diphenyisobenzofuran, and glycine, catalase), whereas mannitol and tryptophan were not effective. The data indicate that electrolysis of a physiological buffer produces a milieu containing several reactive oxygen species or free radicals that have the potential to produce alterations in a biological system. This method has the advantage over existing protocols for the generation of radicals in that it is a blood-free and an enzyme-free system. | en_US |
dc.format.extent | 1847706 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Electrolysis-induced myocardial dysfunction : A novel method for the study of free radical mediated tissue injury | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Pharmacology, University of Michigan Medical School, Ann Arbor, Michigan, USA | en_US |
dc.contributor.affiliationum | Department of Pharmacology, University of Michigan Medical School, Ann Arbor, Michigan, USA | en_US |
dc.contributor.affiliationum | Department of Pharmacology, University of Michigan Medical School, Ann Arbor, Michigan, USA | en_US |
dc.contributor.affiliationum | Department of Pharmacology, University of Michigan Medical School, Ann Arbor, Michigan, USA | en_US |
dc.contributor.affiliationother | Department of Medicine, Montefiore Hospital and Albert Einstein College of Medicine, Bronx, New York (P.S.R.), USA | en_US |
dc.identifier.pmid | 3724201 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/26123/1/0000199.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0160-5402(86)90010-0 | en_US |
dc.identifier.source | Journal of Pharmacological Methods | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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