A microtiter virus yield reduction assay for the evaluation of antiviral compounds against human cytomegalovirus and herpes simplex virus
dc.contributor.author | Prichard, Mark N. | en_US |
dc.contributor.author | Turk, Steven R. | en_US |
dc.contributor.author | Coleman, Lisa A. | en_US |
dc.contributor.author | Engelhardt, Sandra L. | en_US |
dc.contributor.author | Shipman, Charles Jr. | en_US |
dc.contributor.author | Drach, John C. | en_US |
dc.date.accessioned | 2006-04-10T13:46:39Z | |
dc.date.available | 2006-04-10T13:46:39Z | |
dc.date.issued | 1990-04 | en_US |
dc.identifier.citation | Prichard, Mark N., Turk, Steven R., Coleman, Lisa A., Engelhardt, Sandra L., Shipman, Jr., Charles, Drach, John C. (1990/04)."A microtiter virus yield reduction assay for the evaluation of antiviral compounds against human cytomegalovirus and herpes simplex virus." Journal of Virological Methods 28(1): 101-106. <http://hdl.handle.net/2027.42/28636> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T96-476KY1N-VB/2/dadc9253f3c863d3d9cb33e987f9bb3a | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/28636 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2161417&dopt=citation | en_US |
dc.description.abstract | Although the virus yield reduction assay is a powerful technique for evaluating the efficacy of antiviral compounds, it is not routinely utilized due to its labor-intensive nature. This procedure was modified, developed, thereby reducing greatly the time and effort required to perform yield reduction assays. Monolayer cultures of mammalian cells were grown in 96-well microtiter tissue culture plates and infected with virus. Test compounds were added and serially diluted directly with the plates. Following a cycle of virus replication, culture lysates were made and serially diluted in a separate set of uninfected cultures grown in microtiter plates. The cultures were incubated, plaques were enumerated in wells containing 5 to 20 plaques, and virus titers were calculated. To illustrate the use of the assay the known antiviral drugs acyclovir and ganciclovir were evaluated using this procedure. Ninety percent inhibitory concentrations for the respective drugs were 3 [mu]M and 0.7 [mu]M against herpes simplex virus type 1 and 60 [mu]M and 1 [mu]M against human cytomegalovirus. | en_US |
dc.format.extent | 435173 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | A microtiter virus yield reduction assay for the evaluation of antiviral compounds against human cytomegalovirus and herpes simplex virus | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Departments of Microbiology and Immunology, School of Medicine, The University of Michigan, Ann Arbor, Michigan, U.S.A.; Departments of Biologic and Materials Sciences, School of Dentistry, The University of Michigan, Ann Arbor, Michigan, U.S.A. | en_US |
dc.contributor.affiliationum | Departments of Biologic and Materials Sciences, School of Dentistry, The University of Michigan, Ann Arbor, Michigan, U.S.A. | en_US |
dc.contributor.affiliationum | Departments of Biologic and Materials Sciences, School of Dentistry, The University of Michigan, Ann Arbor, Michigan, U.S.A. | en_US |
dc.contributor.affiliationum | Departments of Biologic and Materials Sciences, School of Dentistry, The University of Michigan, Ann Arbor, Michigan, U.S.A. | en_US |
dc.contributor.affiliationum | Departments of Microbiology and Immunology, School of Medicine, The University of Michigan, Ann Arbor, Michigan, U.S.A.; Departments of Biologic and Materials Sciences, School of Dentistry, The University of Michigan, Ann Arbor, Michigan, U.S.A. | en_US |
dc.contributor.affiliationum | Departments of Biologic and Materials Sciences, School of Dentistry, The University of Michigan, Ann Arbor, Michigan, U.S.A. | en_US |
dc.identifier.pmid | 2161417 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/28636/1/0000450.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0166-0934(90)90091-S | en_US |
dc.identifier.source | Journal of Virological Methods | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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