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A method for the quantitation of protamine in plasma

dc.contributor.authorYang, Victor C.en_US
dc.contributor.authorFu, You-Yinen_US
dc.contributor.authorTeng, Ching-Leou C.en_US
dc.contributor.authorMa, Shu-Chingen_US
dc.contributor.authorShanberge, Jacob N.en_US
dc.date.accessioned2006-04-10T18:08:58Z
dc.date.available2006-04-10T18:08:58Z
dc.date.issued1994-05-15en_US
dc.identifier.citationYang, Victor C., Fu, You-Yin, Teng, Ching-Leou C., Ma, Shu-Ching, Shanberge, Jacob N. (1994/05/15)."A method for the quantitation of protamine in plasma." Thrombosis Research 74(4): 427-434. <http://hdl.handle.net/2027.42/31577>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6T1C-4C3C51B-1R/2/d16effb630c6b47051677f788fe2651een_US
dc.identifier.urihttps://hdl.handle.net/2027.42/31577
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7521974&dopt=citationen_US
dc.description.abstractA unique and simple colorimetric method for the quantitation of plasma protamine levels has been developed. The method is established on the competitive binding displacement mechanism between protamine and heparin-azure A dye complex, and the metachromatic color change of azure A dye in the presence of heparin. Because the method is based on the clinical specificity of protamine as the heparin antagonist, it is specific for protamine quantitation. Plasma protamine levels determined by this method are within 94% of accuracy when compared with their aqueous counterparts determined by the conventional Lowry protein assay. Since the method measures the protamine excess after heparin neutralization, it potentially could be employed during clinical heparin reversal with protamine to monitor protamine excess. In addition, the method may provide a useful means to identify the mechanism of the so-called "heparin rebound".en_US
dc.format.extent812623 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titleA method for the quantitation of protamine in plasmaen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelInternal Medicine and Specialtiesen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumCollege of Pharmacy, The University of Michigan, Ann Arbor, MI 48109-1065, USAen_US
dc.contributor.affiliationumCollege of Pharmacy, The University of Michigan, Ann Arbor, MI 48109-1065, USAen_US
dc.contributor.affiliationumCollege of Pharmacy, The University of Michigan, Ann Arbor, MI 48109-1065, USAen_US
dc.contributor.affiliationumNova Biomedical Corp., 200 Prospect Street, Waltham, MA 02254, USA; College of Pharmacy, The University of Michigan, Ann Arbor, MI 48109-1065, USAen_US
dc.contributor.affiliationotherHemostasis and Coagulation Laboratory, William Beaumont Hospital, Royal Oak, MI 48072, USAen_US
dc.identifier.pmid7521974en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/31577/1/0000505.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0049-3848(94)90158-9en_US
dc.identifier.sourceThrombosis Researchen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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