A method for the quantitation of protamine in plasma
dc.contributor.author | Yang, Victor C. | en_US |
dc.contributor.author | Fu, You-Yin | en_US |
dc.contributor.author | Teng, Ching-Leou C. | en_US |
dc.contributor.author | Ma, Shu-Ching | en_US |
dc.contributor.author | Shanberge, Jacob N. | en_US |
dc.date.accessioned | 2006-04-10T18:08:58Z | |
dc.date.available | 2006-04-10T18:08:58Z | |
dc.date.issued | 1994-05-15 | en_US |
dc.identifier.citation | Yang, Victor C., Fu, You-Yin, Teng, Ching-Leou C., Ma, Shu-Ching, Shanberge, Jacob N. (1994/05/15)."A method for the quantitation of protamine in plasma." Thrombosis Research 74(4): 427-434. <http://hdl.handle.net/2027.42/31577> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T1C-4C3C51B-1R/2/d16effb630c6b47051677f788fe2651e | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/31577 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7521974&dopt=citation | en_US |
dc.description.abstract | A unique and simple colorimetric method for the quantitation of plasma protamine levels has been developed. The method is established on the competitive binding displacement mechanism between protamine and heparin-azure A dye complex, and the metachromatic color change of azure A dye in the presence of heparin. Because the method is based on the clinical specificity of protamine as the heparin antagonist, it is specific for protamine quantitation. Plasma protamine levels determined by this method are within 94% of accuracy when compared with their aqueous counterparts determined by the conventional Lowry protein assay. Since the method measures the protamine excess after heparin neutralization, it potentially could be employed during clinical heparin reversal with protamine to monitor protamine excess. In addition, the method may provide a useful means to identify the mechanism of the so-called "heparin rebound". | en_US |
dc.format.extent | 812623 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | A method for the quantitation of protamine in plasma | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Internal Medicine and Specialties | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | College of Pharmacy, The University of Michigan, Ann Arbor, MI 48109-1065, USA | en_US |
dc.contributor.affiliationum | College of Pharmacy, The University of Michigan, Ann Arbor, MI 48109-1065, USA | en_US |
dc.contributor.affiliationum | College of Pharmacy, The University of Michigan, Ann Arbor, MI 48109-1065, USA | en_US |
dc.contributor.affiliationum | Nova Biomedical Corp., 200 Prospect Street, Waltham, MA 02254, USA; College of Pharmacy, The University of Michigan, Ann Arbor, MI 48109-1065, USA | en_US |
dc.contributor.affiliationother | Hemostasis and Coagulation Laboratory, William Beaumont Hospital, Royal Oak, MI 48072, USA | en_US |
dc.identifier.pmid | 7521974 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/31577/1/0000505.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0049-3848(94)90158-9 | en_US |
dc.identifier.source | Thrombosis Research | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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