Protein-carbohydrate interaction : VIII. A turbidimetric method for the analysis of -mannose

Abstract

A unique, spectrophotometric method for the quantitative of -mannose in the presence of other sugars is described. The procedure depends on the degree to which the turbidity formed on interaction of concanavalin A with glycogen is specifically inhibited by -mannose. The analysis, whose useful is 300 to 750 [mu]g/ml, gives essentially the same result as other methods commonly used. A correction for the presence of -glucose, which also inhibits glycogen-concanavalin A interaction, is readily achieved by use of the Glucostat reagent.