A pH- and ionic strength-responsive polypeptide hydrogel: Synthesis, characterization, and preliminary protein release studies
dc.contributor.author | Markland, Peter | en_US |
dc.contributor.author | Zhang, Yuehua | en_US |
dc.contributor.author | Amidon, Gordon L. | en_US |
dc.contributor.author | Yang, Victor C. | en_US |
dc.date.accessioned | 2006-04-19T13:33:19Z | |
dc.date.available | 2006-04-19T13:33:19Z | |
dc.date.issued | 1999-12-15 | en_US |
dc.identifier.citation | Markland, Peter; Zhang, Yuehua; Amidon, Gordon L.; Yang, Victor C. (1999)."A pH- and ionic strength-responsive polypeptide hydrogel: Synthesis, characterization, and preliminary protein release studies." Journal of Biomedical Materials Research 47(4): 595-602. <http://hdl.handle.net/2027.42/34416> | en_US |
dc.identifier.issn | 0021-9304 | en_US |
dc.identifier.issn | 1097-4636 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/34416 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=10497296&dopt=citation | en_US |
dc.description.abstract | A novel polypeptide hydrogel has been synthesized by crosslinking poly(L-glutamic acid) (PLG) with poly(ethylene glycol) (PEG). The PLG-PEG hydrogel was shown to be highly hydrophilic, and the extent of swelling varied with pH, increasing at higher ionization of the PLG. Aside from electrostatic effects, such as ion–ion repulsion and internal ion osmotic pressure, circular dichroism studies showed that swelling response to pH also is affected by secondary structural attributes associated with the polypeptide backbone. Modification of the polypeptide by changing its hydrophobicity and degree of ionization was an effective method for altering the overall extent of pH-responsive swelling. Rapid de-swelling (contraction) was observed when the PLG-PEG hydrogel was transferred from high to low pH buffer solution, and this swelling/de-swelling behavior was reversible over repeated cycles. Drug release from swollen hydrogels was examined using the model protein lysozyme. Rapid de-swelling of the hydrogel was found to be an effective means of facilitating lysozyme release. The crosslinking of synthetic polypeptides with PEG appears to be a highly versatile approach to the preparation of pH-responsive biodegradable hydrogels. © 1999 John Wiley & Sons, Inc. J Biomed Mater Res, 47, 595–602, 1999. | en_US |
dc.format.extent | 223138 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | John Wiley & Sons, Inc. | en_US |
dc.subject.other | Chemistry | en_US |
dc.subject.other | Polymer and Materials Science | en_US |
dc.title | A pH- and ionic strength-responsive polypeptide hydrogel: Synthesis, characterization, and preliminary protein release studies | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbsecondlevel | Biomedical Engineering | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | College of Pharmacy, The University of Michigan, 428 Church Street, Ann Arbor, Michigan 48109-1065 | en_US |
dc.contributor.affiliationum | College of Pharmacy, The University of Michigan, 428 Church Street, Ann Arbor, Michigan 48109-1065 | en_US |
dc.contributor.affiliationum | College of Pharmacy, The University of Michigan, 428 Church Street, Ann Arbor, Michigan 48109-1065 | en_US |
dc.contributor.affiliationum | College of Pharmacy, The University of Michigan, 428 Church Street, Ann Arbor, Michigan 48109-1065 ; College of Pharmacy, The University of Michigan, 428 Church Street, Ann Arbor, Michigan 48109-1065 | en_US |
dc.identifier.pmid | 10497296 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/34416/1/17_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/(SICI)1097-4636(19991215)47:4<595::AID-JBM17>3.0.CO;2-I | en_US |
dc.identifier.source | Journal of Biomedical Materials Research | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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