Engineering new bone tissue in vitro on highly porous poly(Α-hydroxyl acids)/hydroxyapatite composite scaffolds
dc.contributor.author | Ma, Peter X. | en_US |
dc.contributor.author | Zhang, Ruiyun | en_US |
dc.contributor.author | Xiao, Guozhi | en_US |
dc.contributor.author | Franceschi, Renny T. | en_US |
dc.date.accessioned | 2006-04-19T13:33:28Z | |
dc.date.available | 2006-04-19T13:33:28Z | |
dc.date.issued | 2001-02 | en_US |
dc.identifier.citation | Ma, Peter X.; Zhang, Ruiyun; Xiao, Guozhi; Franceschi, Renny (2001)."Engineering new bone tissue in vitro on highly porous poly(Α-hydroxyl acids)/hydroxyapatite composite scaffolds." Journal of Biomedical Materials Research 54(2): 284-293. <http://hdl.handle.net/2027.42/34419> | en_US |
dc.identifier.issn | 0021-9304 | en_US |
dc.identifier.issn | 1097-4636 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/34419 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=11093189&dopt=citation | en_US |
dc.description.abstract | Engineering new bone tissue with cells and a synthetic extracellular matrix (scaffolding) represents a new approach for the regeneration of mineralized tissues compared with the transplantation of bone (autografts or allografts). In the present work, highly porous poly( L -lactic acid) (PLLA) and PLLA/hydroxyapatite (HAP) composite scaffolds were prepared with a thermally induced phase separation technique. The scaffolds were seeded with osteoblastic cells and cultured in vitro . In the pure PLLA scaffolds, the osteoblasts attached primarily on the outer surface of the polymer. In contrast, the osteoblasts penetrated deep into the PLLA/HAP scaffolds and were uniformly distributed. The osteoblast survival percentage in the PLLA/HAP scaffolds was superior to that in the PLLA scaffolds. The osteoblasts proliferated in both types of the scaffolds, but the cell number was always higher in the PLLA/HAP composite scaffolds during 6 weeks of in vitro cultivation. Bone-specific markers (mRNAs encoding bone sialoprotein and osteocalcin) were expressed more abundantly in the PLLA/HAP composite scaffolds than in the PLLA scaffolds. The new tissue increased continuously in the PLLA/HAP composite scaffolds, whereas new tissue formed only near the surface of pure PLLA scaffolds. These results demonstrate that HAP imparts osteoconductivity and the highly porous PLLA/HAP composite scaffolds are superior to pure PLLA scaffolds for bone tissue engineering. © 2000 John Wiley & Sons, Inc. J Biomed Mater Res 54: 284–293, 2001 | en_US |
dc.format.extent | 1695230 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | John Wiley & Sons, Inc. | en_US |
dc.subject.other | Chemistry | en_US |
dc.subject.other | Polymer and Materials Science | en_US |
dc.title | Engineering new bone tissue in vitro on highly porous poly(Α-hydroxyl acids)/hydroxyapatite composite scaffolds | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbsecondlevel | Biomedical Engineering | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biologic and Materials Sciences, 1011 North University Ave., Room 2211, University of Michigan, Ann Arbor, Michigan 48109 ; Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan 48109 ; Macromolecular Science and Engineering Center, University of Michigan, Ann Arbor, Michigan 48109 ; Department of Biologic and Materials Sciences, 1011 North University Ave., Room 2211, University of Michigan, Ann Arbor, Michigan 48109 | en_US |
dc.contributor.affiliationum | Department of Biologic and Materials Sciences, 1011 North University Ave., Room 2211, University of Michigan, Ann Arbor, Michigan 48109 | en_US |
dc.contributor.affiliationum | Department of Periodontics/Prevention/Geriatrics, University of Michigan, Ann Arbor, Michigan 48109 | en_US |
dc.contributor.affiliationum | Department of Periodontics/Prevention/Geriatrics, University of Michigan, Ann Arbor, Michigan 48109 | en_US |
dc.identifier.pmid | 11093189 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/34419/1/16_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/1097-4636(200102)54:2<284::AID-JBM16>3.0.CO;2-W | en_US |
dc.identifier.source | Journal of Biomedical Materials Research | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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