Perifollicular transgenic expression of human interleukin-1 receptor antagonist protein following topical application of novel liposome-plasmid dna formulations in vivo
dc.contributor.author | Niemiec, Susan M. | en_US |
dc.contributor.author | Latta, Jill M. | en_US |
dc.contributor.author | Ramachandran, Chandrasekharan | en_US |
dc.contributor.author | Weiner, Norman D. | en_US |
dc.contributor.author | Roessler, Blake J. | en_US |
dc.date.accessioned | 2006-04-19T13:36:49Z | |
dc.date.available | 2006-04-19T13:36:49Z | |
dc.date.issued | 1997-06 | en_US |
dc.identifier.citation | Niemiec, Susan M.; Latta, Jill M.; Ramachandran, Chandrasekharan; Weiner, Norman D.; Roessler, Blake J. (1997)."Perifollicular transgenic expression of human interleukin-1 receptor antagonist protein following topical application of novel liposome-plasmid dna formulations in vivo ." Journal of Pharmaceutical Sciences 86(6): 701-708. <http://hdl.handle.net/2027.42/34491> | en_US |
dc.identifier.issn | 0022-3549 | en_US |
dc.identifier.issn | 1520-6017 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/34491 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=9188052&dopt=citation | en_US |
dc.description.abstract | Expression plasmid DNA for the human interleukin-1 receptor antagonist (IL-1ra) protein was formulated with nonionic:cationic (NC) liposomes or phosphatidylcholine:cationic (PC) liposomes and applied to the auricular skin of hamsters in single- and multiple-dose protocols. Confocal microscopy identified delivery of plasmid DNA proximal to perifollicular cells, and successful transfection of perifollicular cells was identified by immunohistochemistry and ELISA. Skin treated for 3 days with the NC liposomes had statistically significant levels of transgenic IL-1ra present for 5 days post-treatment. Expression of transgenic IL-1ra was specific to areas of skin treated with NC liposomes but not PC liposomes. The results indicate that the NC liposomes can deliver expression plasmid DNA to perifollicular cells and mediate transient transfection in vivo . | en_US |
dc.format.extent | 287313 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | John Wiley & Sons, Inc. | en_US |
dc.subject.other | Chemistry | en_US |
dc.subject.other | Food Science, Agricultural, Medicinal and Pharmaceutical Chemistry | en_US |
dc.title | Perifollicular transgenic expression of human interleukin-1 receptor antagonist protein following topical application of novel liposome-plasmid dna formulations in vivo | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Pharmacy and Pharmacology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | College of Pharmacy, University of Michigan, Ann Arbor, MI 48109-1065. | en_US |
dc.contributor.affiliationum | Department of Internal Medicine, Division of Rheumatology, 5520 MSRB I, Box 0608, 1150 West Medical Center Drive, University of Michigan Medical Center, Ann Arbor, MI 48109-0680. | en_US |
dc.contributor.affiliationum | College of Pharmacy, University of Michigan, Ann Arbor, MI 48109-1065. | en_US |
dc.contributor.affiliationum | College of Pharmacy, University of Michigan, Ann Arbor, MI 48109-1065. | en_US |
dc.contributor.affiliationum | College of Pharmacy, University of Michigan, Ann Arbor, MI 48109-1065. ; Department of Internal Medicine, Division of Rheumatology, 5520 MSRB I, Box 0608, 1150 West Medical Center Drive, University of Michigan Medical Center, Ann Arbor, MI 48109-0680. ; College of Pharmacy, University of Michigan, Ann Arbor, MI 48109-1065. | en_US |
dc.identifier.pmid | 9188052 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/34491/1/8_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/(ISSN)1520-6017 | en_US |
dc.identifier.source | Journal of Pharmaceutical Sciences | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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