Unilineage model of hematopoiesis predicts self-renewal of stem and progenitor cells based on ex vivo growth data
dc.contributor.author | Peng, Ching-An | en_US |
dc.contributor.author | Koller, Manfred R. | en_US |
dc.contributor.author | Palsson, Bernhard Ø. | en_US |
dc.date.accessioned | 2006-04-28T16:32:07Z | |
dc.date.available | 2006-04-28T16:32:07Z | |
dc.date.issued | 1996-10-05 | en_US |
dc.identifier.citation | Peng, Ching-An; Koller, Manfred R.; Palsson, Bernhard Ø. (1996)."Unilineage model of hematopoiesis predicts self-renewal of stem and progenitor cells based on ex vivo growth data." Biotechnology and Bioengineering 52(1): 24-33. <http://hdl.handle.net/2027.42/37939> | en_US |
dc.identifier.issn | 0006-3592 | en_US |
dc.identifier.issn | 1097-0290 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/37939 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=18629849&dopt=citation | en_US |
dc.description.abstract | Stem cell models are used to describe the function of several tissues. We present unilineage kinetic description of stem cell models and their application to the analysis of ex vivo hematopoietic cell expansion data. This model has the capability to simulate the total cell number and the number of cells at each stage of differentiation over time as a function of the stem cell self-renewal probability, the growth rate of each subpopulation, and the mature cell death rate. The model predicts experimental observations in perfusion-based hematopoietic bioreactor systems. To obtain net cell expansion ex vivo, the model simulations show that the stem cell self-renewal probability must exceed one-half, thus resulting in net expansion of the stem cell population. Experimental data on long-term culture-initiating cells (LTC-IC) confirm this prediction and the probability of self-renewal is estimated to be 0.62 to 0.73. This self-renewal probability, along with the death rate, define a relationship in which the apparent overall growth rate is less than the compartmental growth rate. Finally, the model predicts that cells beyond the stem cell stage of differentiation must self-renew to achieve the level of expansion within the time frame observed in experimental systems. © 1996 John Wiley & Sons, Inc. | en_US |
dc.format.extent | 940733 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.subject.other | Chemistry | en_US |
dc.subject.other | Biochemistry and Biotechnology | en_US |
dc.title | Unilineage model of hematopoiesis predicts self-renewal of stem and progenitor cells based on ex vivo growth data | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbsecondlevel | Mathematics | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Statistics and Numeric Data | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Social Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Chemical Engineering, University of Michigan, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Department of Chemical Engineering, University of Michigan, Ann Arbor, Michigan ; Aastrom Biosciences, Inc., Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Department of Chemical Engineering, University of Michigan, Ann Arbor, Michigan ; Department of Bioengineering, 9500 Gilman Drive, Mail Code 0412, University of California at San Diego, La Jolla, CA 92093-0412. Telephone: 619-534-5668; fax: 619-822-0240 | en_US |
dc.identifier.pmid | 18629849 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/37939/1/3_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/(SICI)1097-0290(19961005)52:1<24::AID-BIT3>3.0.CO;2-0 | en_US |
dc.identifier.source | Biotechnology and Bioengineering | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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