Aquifex aeolicus 3-Deoxy- d - manno -2-Octulosonic Acid 8-Phosphate Synthase: A New Class of KDO 8-P Synthase?
dc.contributor.author | Birck, Matthew R. | en_US |
dc.contributor.author | Woodard, Ronald W. | en_US |
dc.date.accessioned | 2006-09-08T20:16:03Z | |
dc.date.available | 2006-09-08T20:16:03Z | |
dc.date.issued | 2001-02 | en_US |
dc.identifier.citation | Birck, Matthew R.; Woodard, Ronald W.; (2001). " Aquifex aeolicus 3-Deoxy- d - manno -2-Octulosonic Acid 8-Phosphate Synthase: A New Class of KDO 8-P Synthase?." Journal of Molecular Evolution 52(2): 205-214. <http://hdl.handle.net/2027.42/42370> | en_US |
dc.identifier.issn | 0022-2844 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/42370 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=11244581&dopt=citation | en_US |
dc.description.abstract | The relationship between 3-deoxy- d - manno 2-octulosonic acid 8-phosphate (KDO 8-P) synthase and 3-deoxy- d - arabino -2-heptulosonic acid 7-phosphate (DAH 7-P) synthase has not been adequately addressed in the literature. Based on recent reports of a metal requiring KDO 8-P synthase and the newly solved X-ray crystal structures of both Escherichia coli KDO 8-P synthase and DAH 7-P synthase, we begin to address the evolutionary kinship between these catalytically similar enzymes. Using a maximum likelihood-based grouping of 29 KDO 8-P synthase sequences, we demonstrate the existence of a new class of KDO 8-P synthase, the members of which we propose to require a metal cofactor for catalysis. Similarly, we hypothesize a class of DAH 7-P synthase that does not have the metal requirement of the heretofore model E. coli enzyme. Based on this information and a careful investigation of the reported X-ray crystal structures, we also propose that KDO 8-P synthase and DAH 7-P synthase are the product of a divergent evolutionary process from a common ancestor. | en_US |
dc.format.extent | 957983 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Springer-Verlag; Springer-Verlag New York Inc. | en_US |
dc.subject.other | Legacy | en_US |
dc.subject.other | Key Words: Lipopolysaccharide — O-antigen — Shikimate Pathway — Maximum Likelihood | en_US |
dc.title | Aquifex aeolicus 3-Deoxy- d - manno -2-Octulosonic Acid 8-Phosphate Synthase: A New Class of KDO 8-P Synthase? | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Medicinal Chemistry, College of Pharmacy, University of Michigan, 428 Church Street, Ann Arbor, MI 48109-1065, USA, US, | en_US |
dc.contributor.affiliationum | Department of Medicinal Chemistry, College of Pharmacy, University of Michigan, 428 Church Street, Ann Arbor, MI 48109-1065, USA, US, | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 11244581 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/42370/1/239-52-2-205_10520205.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/s002390010149 | en_US |
dc.identifier.source | Journal of Molecular Evolution | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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