Cloning and characterization of Daphnia mitochondrial DNA
Hebert, P. D. N.; Crease, T. J.; Stanton, D. J.
1991-08
Citation
Stanton, D. J.; Crease, T. J.; Hebert, P. D. N.; (1991). "Cloning and characterization of Daphnia mitochondrial DNA." Journal of Molecular Evolution 33(2): 152-155. <http://hdl.handle.net/2027.42/48044>
Abstract
The mitochondrial genome of Daphnia pulex (Crustacea, Cladocera) was cloned as a single fragment into the plasmid vector pUC12. The genome size, estimated from restriction endonuclease fragment lengths, is 15,400±200 base pairs. The GC content, estimated from thermal denaturation studies, is 42%. The positions of 39 cleavage sites were mapped for 14 restriction enzymes. The distribution of these sites within the genome is random ( P =0.44). Heterologous hybridizations with Drosophila sylvestris mitochondrial DNA (mtDNA) probes indicate that gene orders within Daphnia and Drosophila mtDNAs are similar.Publisher
Springer-Verlag; Springer-Verlag New York Inc.
ISSN
0022-2844 1432-1432
Other DOIs
PMID
1920451
Types
Article
Metadata
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