Show simple item record

Cloning and characterization of Daphnia mitochondrial DNA

dc.contributor.authorHebert, P. D. N.en_US
dc.contributor.authorCrease, T. J.en_US
dc.contributor.authorStanton, D. J.en_US
dc.date.accessioned2006-09-11T19:41:43Z
dc.date.available2006-09-11T19:41:43Z
dc.date.issued1991-08en_US
dc.identifier.citationStanton, D. J.; Crease, T. J.; Hebert, P. D. N.; (1991). "Cloning and characterization of Daphnia mitochondrial DNA." Journal of Molecular Evolution 33(2): 152-155. <http://hdl.handle.net/2027.42/48044>en_US
dc.identifier.issn0022-2844en_US
dc.identifier.issn1432-1432en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/48044
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1920451&dopt=citationen_US
dc.description.abstractThe mitochondrial genome of Daphnia pulex (Crustacea, Cladocera) was cloned as a single fragment into the plasmid vector pUC12. The genome size, estimated from restriction endonuclease fragment lengths, is 15,400±200 base pairs. The GC content, estimated from thermal denaturation studies, is 42%. The positions of 39 cleavage sites were mapped for 14 restriction enzymes. The distribution of these sites within the genome is random ( P =0.44). Heterologous hybridizations with Drosophila sylvestris mitochondrial DNA (mtDNA) probes indicate that gene orders within Daphnia and Drosophila mtDNAs are similar.en_US
dc.format.extent376107 bytes
dc.format.extent3115 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherSpringer-Verlag; Springer-Verlag New York Inc.en_US
dc.subject.otherCell Biologyen_US
dc.subject.otherGenome Sizeen_US
dc.subject.otherCladoceraen_US
dc.subject.otherCrustaceaen_US
dc.subject.otherPlant Sciencesen_US
dc.subject.otherGC Contenten_US
dc.subject.otherLife Sciencesen_US
dc.subject.otherMicrobiologyen_US
dc.subject.otherGene Orderen_US
dc.subject.otherRestriction Mappingen_US
dc.titleCloning and characterization of Daphnia mitochondrial DNAen_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelNatural Resources and Environmenten_US
dc.subject.hlbsecondlevelEcology and Evolutionary Biologyen_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Biological Sciences, University of Windsor, N9B 3P4, Windsor, Ontario, Canada; Department of Biology, University of Michigan, 48109, Ann Arbor, MI, USAen_US
dc.contributor.affiliationotherDepartment of Zoology, University of Guelph, N1G 2W1, Guelph, Ontario, Canadaen_US
dc.contributor.affiliationotherDepartment of Biological Sciences, University of Windsor, N9B 3P4, Windsor, Ontario, Canadaen_US
dc.contributor.affiliationumcampusAnn Arboren_US
dc.identifier.pmid1920451en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/48044/1/239_2005_Article_BF02193629.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1007/BF02193629en_US
dc.identifier.sourceJournal of Molecular Evolutionen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


Files in this item

Show simple item record

Accessibility: If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.