Cloning and characterization of Daphnia mitochondrial DNA
dc.contributor.author | Hebert, P. D. N. | en_US |
dc.contributor.author | Crease, T. J. | en_US |
dc.contributor.author | Stanton, D. J. | en_US |
dc.date.accessioned | 2006-09-11T19:41:43Z | |
dc.date.available | 2006-09-11T19:41:43Z | |
dc.date.issued | 1991-08 | en_US |
dc.identifier.citation | Stanton, D. J.; Crease, T. J.; Hebert, P. D. N.; (1991). "Cloning and characterization of Daphnia mitochondrial DNA." Journal of Molecular Evolution 33(2): 152-155. <http://hdl.handle.net/2027.42/48044> | en_US |
dc.identifier.issn | 0022-2844 | en_US |
dc.identifier.issn | 1432-1432 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/48044 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1920451&dopt=citation | en_US |
dc.description.abstract | The mitochondrial genome of Daphnia pulex (Crustacea, Cladocera) was cloned as a single fragment into the plasmid vector pUC12. The genome size, estimated from restriction endonuclease fragment lengths, is 15,400±200 base pairs. The GC content, estimated from thermal denaturation studies, is 42%. The positions of 39 cleavage sites were mapped for 14 restriction enzymes. The distribution of these sites within the genome is random ( P =0.44). Heterologous hybridizations with Drosophila sylvestris mitochondrial DNA (mtDNA) probes indicate that gene orders within Daphnia and Drosophila mtDNAs are similar. | en_US |
dc.format.extent | 376107 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Springer-Verlag; Springer-Verlag New York Inc. | en_US |
dc.subject.other | Cell Biology | en_US |
dc.subject.other | Genome Size | en_US |
dc.subject.other | Cladocera | en_US |
dc.subject.other | Crustacea | en_US |
dc.subject.other | Plant Sciences | en_US |
dc.subject.other | GC Content | en_US |
dc.subject.other | Life Sciences | en_US |
dc.subject.other | Microbiology | en_US |
dc.subject.other | Gene Order | en_US |
dc.subject.other | Restriction Mapping | en_US |
dc.title | Cloning and characterization of Daphnia mitochondrial DNA | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biological Sciences, University of Windsor, N9B 3P4, Windsor, Ontario, Canada; Department of Biology, University of Michigan, 48109, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationother | Department of Zoology, University of Guelph, N1G 2W1, Guelph, Ontario, Canada | en_US |
dc.contributor.affiliationother | Department of Biological Sciences, University of Windsor, N9B 3P4, Windsor, Ontario, Canada | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 1920451 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/48044/1/239_2005_Article_BF02193629.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/BF02193629 | en_US |
dc.identifier.source | Journal of Molecular Evolution | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.