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Cloning and characterization of Daphnia mitochondrial DNA

dc.contributor.authorHebert, P. D. N.en_US
dc.contributor.authorCrease, T. J.en_US
dc.contributor.authorStanton, D. J.en_US
dc.identifier.citationStanton, D. J.; Crease, T. J.; Hebert, P. D. N.; (1991). "Cloning and characterization of Daphnia mitochondrial DNA." Journal of Molecular Evolution 33(2): 152-155. <>en_US
dc.description.abstractThe mitochondrial genome of Daphnia pulex (Crustacea, Cladocera) was cloned as a single fragment into the plasmid vector pUC12. The genome size, estimated from restriction endonuclease fragment lengths, is 15,400±200 base pairs. The GC content, estimated from thermal denaturation studies, is 42%. The positions of 39 cleavage sites were mapped for 14 restriction enzymes. The distribution of these sites within the genome is random ( P =0.44). Heterologous hybridizations with Drosophila sylvestris mitochondrial DNA (mtDNA) probes indicate that gene orders within Daphnia and Drosophila mtDNAs are similar.en_US
dc.format.extent376107 bytes
dc.format.extent3115 bytes
dc.publisherSpringer-Verlag; Springer-Verlag New York Inc.en_US
dc.subject.otherCell Biologyen_US
dc.subject.otherGenome Sizeen_US
dc.subject.otherPlant Sciencesen_US
dc.subject.otherGC Contenten_US
dc.subject.otherLife Sciencesen_US
dc.subject.otherGene Orderen_US
dc.subject.otherRestriction Mappingen_US
dc.titleCloning and characterization of Daphnia mitochondrial DNAen_US
dc.subject.hlbsecondlevelNatural Resources and Environmenten_US
dc.subject.hlbsecondlevelEcology and Evolutionary Biologyen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Biological Sciences, University of Windsor, N9B 3P4, Windsor, Ontario, Canada; Department of Biology, University of Michigan, 48109, Ann Arbor, MI, USAen_US
dc.contributor.affiliationotherDepartment of Zoology, University of Guelph, N1G 2W1, Guelph, Ontario, Canadaen_US
dc.contributor.affiliationotherDepartment of Biological Sciences, University of Windsor, N9B 3P4, Windsor, Ontario, Canadaen_US
dc.contributor.affiliationumcampusAnn Arboren_US
dc.identifier.sourceJournal of Molecular Evolutionen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed

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