Microscale sample deposition onto hydrophobic target plates for trace level detection of neuropeptides in brain tissue by MALDI-MS
dc.contributor.author | Wei, Hui | en_US |
dc.contributor.author | Dean, Stacey L. | en_US |
dc.contributor.author | Parkin, Mark C. | en_US |
dc.contributor.author | Nolkrantz, Kerstin | en_US |
dc.contributor.author | O'Callaghan, James P. | en_US |
dc.contributor.author | Kennedy, Robert T. | en_US |
dc.date.accessioned | 2006-12-07T16:52:55Z | |
dc.date.available | 2006-12-07T16:52:55Z | |
dc.date.issued | 2005-10 | en_US |
dc.identifier.citation | Wei, Hui; Dean, Stacey L.; Parkin, Mark C.; Nolkrantz, Kerstin; O'Callaghan, James P.; Kennedy, Robert T. (2005)."Microscale sample deposition onto hydrophobic target plates for trace level detection of neuropeptides in brain tissue by MALDI-MS." Journal of Mass Spectrometry 40(10): 1338-1346. <http://hdl.handle.net/2027.42/48772> | en_US |
dc.identifier.issn | 1076-5174 | en_US |
dc.identifier.issn | 1096-9888 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/48772 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=16217843&dopt=citation | en_US |
dc.description.abstract | A sample preparation method that combines a modified target plate with a nanoscale reversed-phase column (nanocolumn) was developed for detection of neuropeptides by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). A gold-coated MALDI plate was modified with an octadecanethiol (ODT) self-assembled monolayer to create a hydrophobic surface that could concentrate peptide samples into a ∼200–500-µm diameter spot. The spot sizes generated were comparable to those obtained for a substrate patterned with 200-µm hydrophilic spots on a hydrophobic substrate. The sample spots on the ODT-coated plate were 100-fold smaller than those formed on an unmodified gold plate with a 1-µl sample and generated 10 to 50 times higher mass sensitivity for peptide standards by MALDI-TOF MS. When the sample was deposited on an ODT-modified plate from a nanocolumn, the detection limit for peptides was as low as 20 pM for 5-µl samples corresponding to 80 amol deposited. This technique was used to analyze extracts of microwave-fixed tissue from rat brain striatum. Ninety-eight putative peptides were detected including several that had masses matching neuropeptides expected in this brain region such as substance P, rimorphin, and neurotensin. Twenty-three peptides had masses that matched peaks detected by capillary liquid chromatography with electrospray ionization MS. 1 2 Copyright © 2005 John Wiley & Sons, Ltd. | en_US |
dc.format.extent | 564697 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | John Wiley & Sons, Ltd. | en_US |
dc.subject.other | Chemistry | en_US |
dc.subject.other | Analytical Chemistry and Spectroscopy | en_US |
dc.title | Microscale sample deposition onto hydrophobic target plates for trace level detection of neuropeptides in brain tissue by MALDI-MS | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Chemistry, University of Michigan, Ann Arbor, MI, 48109, USA | en_US |
dc.contributor.affiliationum | Department of Chemistry, University of Michigan, Ann Arbor, MI, 48109, USA | en_US |
dc.contributor.affiliationum | Department of Chemistry, University of Michigan, Ann Arbor, MI, 48109, USA | en_US |
dc.contributor.affiliationum | Department of Chemistry, University of Michigan, Ann Arbor, MI, 48109, USA | en_US |
dc.contributor.affiliationum | Department of Chemistry, University of Michigan, Ann Arbor, MI, 48109, USA ; Department of Pharmacology, University of Michigan, Ann Arbor, MI, 48109, USA ; Department of Chemistry, University of Michigan, 930 North University Avenue, Ann Arbor, MI 48109-1055, USA. | en_US |
dc.contributor.affiliationother | HELD/TMBB, Centers for Disease Control and Prevention, Morgantown, West Virginia 26505, USA | en_US |
dc.identifier.pmid | 16217843 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/48772/1/916_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/jms.916 | en_US |
dc.identifier.source | Journal of Mass Spectrometry | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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