Regulation of insulin-like growth factor-II expression and its role in autocrine growth of human neuroblastoma cells
Martin, Donna M.; Feldman, Eva L.
1993-05
Citation
Martin, D. M.; Feldman, E. L. (1993)."Regulation of insulin-like growth factor-II expression and its role in autocrine growth of human neuroblastoma cells." Journal of Cellular Physiology 155(2): 290-300. <http://hdl.handle.net/2027.42/49884>
Abstract
Insulin-like growth factor-II (IGF-II) is highly expressed in fetal tissues and may act as an autocrine growth factor during early embryogenesis. The SH-SY5Y human neuroblastoma cell line also expresses IGF-II and its receptors and responds to exogenous IGF-II with increased DNA synthesis, cell division, and neuritic outgrowth. For this study, we tested the hypothesis that IGF-II mediates autocrine growth of SH-SY5Y cells in serum-free media. SH-SY5Y cells plated at high densities proliferated in serum-free media, whereas sparsely plated cells did not. IGF-II mRNA levels increased within 24 hours of serum deprivation and were associated with increased immunoreactive IGF-II protein. Exogenous addition of IGF-II increased 3 H-TdR incorporation and cell number in a dose- and time-dependent fashion. By nuclear labelling experiments using 5-Bromo-2′ deoxyuridine (BrdU), we detected a twofold higher percentage of S phase nuclei after a 24-hour incubation in IGF-II. Treatment of SH-SY5Y cells with anti-IGF-II antibodies in serum-free media inhibited cell proliferation, and this inhibition was partially overcome by the addition of increasing concentrations of IGF-II. Collectively, our results indicate that IGF-II mediates an autocrine growth mechanism in SH-SY5Y cells that is associated with increased IGF-II expression. © 1993 Wiley-Liss, Inc.Publisher
Wiley Subscription Services, Inc., A Wiley Company
ISSN
0021-9541 1097-4652
Other DOIs
PMID
8482722
Types
Article
Metadata
Show full item recordAccessibility: If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.