Regulation of insulin-like growth factor-II expression and its role in autocrine growth of human neuroblastoma cells
dc.contributor.author | Martin, Donna M. | en_US |
dc.contributor.author | Feldman, Eva L. | en_US |
dc.date.accessioned | 2007-04-06T18:04:38Z | |
dc.date.available | 2007-04-06T18:04:38Z | |
dc.date.issued | 1993-05 | en_US |
dc.identifier.citation | Martin, D. M.; Feldman, E. L. (1993)."Regulation of insulin-like growth factor-II expression and its role in autocrine growth of human neuroblastoma cells." Journal of Cellular Physiology 155(2): 290-300. <http://hdl.handle.net/2027.42/49884> | en_US |
dc.identifier.issn | 0021-9541 | en_US |
dc.identifier.issn | 1097-4652 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/49884 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=8482722&dopt=citation | en_US |
dc.description.abstract | Insulin-like growth factor-II (IGF-II) is highly expressed in fetal tissues and may act as an autocrine growth factor during early embryogenesis. The SH-SY5Y human neuroblastoma cell line also expresses IGF-II and its receptors and responds to exogenous IGF-II with increased DNA synthesis, cell division, and neuritic outgrowth. For this study, we tested the hypothesis that IGF-II mediates autocrine growth of SH-SY5Y cells in serum-free media. SH-SY5Y cells plated at high densities proliferated in serum-free media, whereas sparsely plated cells did not. IGF-II mRNA levels increased within 24 hours of serum deprivation and were associated with increased immunoreactive IGF-II protein. Exogenous addition of IGF-II increased 3 H-TdR incorporation and cell number in a dose- and time-dependent fashion. By nuclear labelling experiments using 5-Bromo-2′ deoxyuridine (BrdU), we detected a twofold higher percentage of S phase nuclei after a 24-hour incubation in IGF-II. Treatment of SH-SY5Y cells with anti-IGF-II antibodies in serum-free media inhibited cell proliferation, and this inhibition was partially overcome by the addition of increasing concentrations of IGF-II. Collectively, our results indicate that IGF-II mediates an autocrine growth mechanism in SH-SY5Y cells that is associated with increased IGF-II expression. © 1993 Wiley-Liss, Inc. | en_US |
dc.format.extent | 1254507 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Cell & Developmental Biology | en_US |
dc.title | Regulation of insulin-like growth factor-II expression and its role in autocrine growth of human neuroblastoma cells | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Kinesiology and Sports | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Neurology and the Neuroscience Program, University of Michigan, Ann Arbor, Michigan 48104 | en_US |
dc.contributor.affiliationum | Department of Neurology and the Neuroscience Program, University of Michigan, Ann Arbor, Michigan 48104 ; Department of Neurology and the Neuroscience Program, University of Michigan, Ann Arbor, Michigan 48104 | en_US |
dc.identifier.pmid | 8482722 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/49884/1/1041550210_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/jcp.1041550210 | en_US |
dc.identifier.source | Journal of Cellular Physiology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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