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dc.contributor.authorMartin, Donna M.en_US
dc.contributor.authorFeldman, Eva L.en_US
dc.date.accessioned2007-04-06T18:04:38Z
dc.date.available2007-04-06T18:04:38Z
dc.date.issued1993-05en_US
dc.identifier.citationMartin, D. M.; Feldman, E. L. (1993)."Regulation of insulin-like growth factor-II expression and its role in autocrine growth of human neuroblastoma cells." Journal of Cellular Physiology 155(2): 290-300. <http://hdl.handle.net/2027.42/49884>en_US
dc.identifier.issn0021-9541en_US
dc.identifier.issn1097-4652en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/49884
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=8482722&dopt=citationen_US
dc.description.abstractInsulin-like growth factor-II (IGF-II) is highly expressed in fetal tissues and may act as an autocrine growth factor during early embryogenesis. The SH-SY5Y human neuroblastoma cell line also expresses IGF-II and its receptors and responds to exogenous IGF-II with increased DNA synthesis, cell division, and neuritic outgrowth. For this study, we tested the hypothesis that IGF-II mediates autocrine growth of SH-SY5Y cells in serum-free media. SH-SY5Y cells plated at high densities proliferated in serum-free media, whereas sparsely plated cells did not. IGF-II mRNA levels increased within 24 hours of serum deprivation and were associated with increased immunoreactive IGF-II protein. Exogenous addition of IGF-II increased 3 H-TdR incorporation and cell number in a dose- and time-dependent fashion. By nuclear labelling experiments using 5-Bromo-2′ deoxyuridine (BrdU), we detected a twofold higher percentage of S phase nuclei after a 24-hour incubation in IGF-II. Treatment of SH-SY5Y cells with anti-IGF-II antibodies in serum-free media inhibited cell proliferation, and this inhibition was partially overcome by the addition of increasing concentrations of IGF-II. Collectively, our results indicate that IGF-II mediates an autocrine growth mechanism in SH-SY5Y cells that is associated with increased IGF-II expression. © 1993 Wiley-Liss, Inc.en_US
dc.format.extent1254507 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.publisherWiley Subscription Services, Inc., A Wiley Companyen_US
dc.subject.otherLife and Medical Sciencesen_US
dc.subject.otherCell & Developmental Biologyen_US
dc.titleRegulation of insulin-like growth factor-II expression and its role in autocrine growth of human neuroblastoma cellsen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbsecondlevelKinesiology and Sportsen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Neurology and the Neuroscience Program, University of Michigan, Ann Arbor, Michigan 48104en_US
dc.contributor.affiliationumDepartment of Neurology and the Neuroscience Program, University of Michigan, Ann Arbor, Michigan 48104 ; Department of Neurology and the Neuroscience Program, University of Michigan, Ann Arbor, Michigan 48104en_US
dc.identifier.pmid8482722en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/49884/1/1041550210_ftp.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1002/jcp.1041550210en_US
dc.identifier.sourceJournal of Cellular Physiologyen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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