Temporal pattern of C1q deposition after transient focal cerebral ischemia
Mack, William J.; Sughrue, Michael E.; Ducruet, Andrew F.; Mocco, J.; Sosunov, Sergey A.; Hassid, Benjamin G.; Silverberg, Joshua Z.; Ten, Vadim S.; Pinsky, David J.; Connolly, E. Sander
2006-04
Citation
Mack, William J.; Sughrue, Michael E.; Ducruet, Andrew F.; Mocco, J; Sosunov, Sergey A.; Hassid, Benjamin G.; Silverberg, Joshua Z.; Ten, Vadim S.; Pinsky, David J.; Connolly, E. Sander (2006). "Temporal pattern of C1q deposition after transient focal cerebral ischemia." Journal of Neuroscience Research 83(5): 883-889. <http://hdl.handle.net/2027.42/50651>
Abstract
Recent studies have focused on elucidating the contribution of individual complement proteins to post-ischemic cellular injury. As the timing of complement activation and deposition after cerebral ischemia is not well understood, our study investigates the temporal pattern of C1q accumulation after experimental murine stroke. Brains were harvested from mice subjected to transient focal cerebral ischemia at 3, 6, 12, and 24 hr post reperfusion. Western blotting and light microscopy were employed to determine the temporal course of C1q protein accumulation and correlate this sequence with infarct evolution observed with TTC staining. Confocal microscopy was utilized to further characterize the cellular localization and characteristics of C1q deposition. Western Blot analysis showed that C1q protein begins to accumulate in the ischemic hemisphere between 3 and 6 hr post-ischemia. Light microscopy confirmed these findings, showing concurrent C1q protein staining of neurons. Confocal microscopy demonstrated co-localization of C1q protein with neuronal cell bodies as well as necrotic cellular debris. These experiments demonstrate the accumulation of C1q protein on neurons during the period of greatest infarct evolution. This data provides information regarding the optimal time window during which a potentially neuroprotective anti-C1q strategy is most likely to achieve therapeutic success. © 2006 Wiley-Liss, Inc.Publisher
Wiley Subscription Services, Inc., A Wiley Company
ISSN
0360-4012 1097-4547
Other DOIs
PMID
16447284
Types
Article
URI
http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=16447284&dopt=citationMetadata
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