Show simple item record

Temporal pattern of C1q deposition after transient focal cerebral ischemia

dc.contributor.authorMack, William J.en_US
dc.contributor.authorSughrue, Michael E.en_US
dc.contributor.authorDucruet, Andrew F.en_US
dc.contributor.authorMocco, J.en_US
dc.contributor.authorSosunov, Sergey A.en_US
dc.contributor.authorHassid, Benjamin G.en_US
dc.contributor.authorSilverberg, Joshua Z.en_US
dc.contributor.authorTen, Vadim S.en_US
dc.contributor.authorPinsky, David J.en_US
dc.contributor.authorConnolly, E. Sanderen_US
dc.date.accessioned2007-05-02T14:16:17Z
dc.date.available2007-05-02T14:16:17Z
dc.date.issued2006-04en_US
dc.identifier.citationMack, William J.; Sughrue, Michael E.; Ducruet, Andrew F.; Mocco, J; Sosunov, Sergey A.; Hassid, Benjamin G.; Silverberg, Joshua Z.; Ten, Vadim S.; Pinsky, David J.; Connolly, E. Sander (2006). "Temporal pattern of C1q deposition after transient focal cerebral ischemia." Journal of Neuroscience Research 83(5): 883-889. <http://hdl.handle.net/2027.42/50651>en_US
dc.identifier.issn0360-4012en_US
dc.identifier.issn1097-4547en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/50651
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=16447284&dopt=citationen_US
dc.description.abstractRecent studies have focused on elucidating the contribution of individual complement proteins to post-ischemic cellular injury. As the timing of complement activation and deposition after cerebral ischemia is not well understood, our study investigates the temporal pattern of C1q accumulation after experimental murine stroke. Brains were harvested from mice subjected to transient focal cerebral ischemia at 3, 6, 12, and 24 hr post reperfusion. Western blotting and light microscopy were employed to determine the temporal course of C1q protein accumulation and correlate this sequence with infarct evolution observed with TTC staining. Confocal microscopy was utilized to further characterize the cellular localization and characteristics of C1q deposition. Western Blot analysis showed that C1q protein begins to accumulate in the ischemic hemisphere between 3 and 6 hr post-ischemia. Light microscopy confirmed these findings, showing concurrent C1q protein staining of neurons. Confocal microscopy demonstrated co-localization of C1q protein with neuronal cell bodies as well as necrotic cellular debris. These experiments demonstrate the accumulation of C1q protein on neurons during the period of greatest infarct evolution. This data provides information regarding the optimal time window during which a potentially neuroprotective anti-C1q strategy is most likely to achieve therapeutic success. © 2006 Wiley-Liss, Inc.en_US
dc.format.extent1237241 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.publisherWiley Subscription Services, Inc., A Wiley Companyen_US
dc.subject.otherLife and Medical Sciencesen_US
dc.subject.otherNeuroscience, Neurology and Psychiatryen_US
dc.titleTemporal pattern of C1q deposition after transient focal cerebral ischemiaen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbsecondlevelNeurosciencesen_US
dc.subject.hlbsecondlevelPsychologyen_US
dc.subject.hlbsecondlevelPublic Healthen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelSocial Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDivision of Cardiology, Department of Medicine, University of Michigan, Ann Arbor, Michiganen_US
dc.contributor.affiliationotherDepartment of Neurological Surgery, College of Physicians & Surgeons, Columbia University, New York, New York ; Dept. of Neurological Surgery, Columbia University, 710 West 168th Street, New York, NY 10032en_US
dc.contributor.affiliationotherDepartment of Neurological Surgery, College of Physicians & Surgeons, Columbia University, New York, New Yorken_US
dc.contributor.affiliationotherDepartment of Neurological Surgery, College of Physicians & Surgeons, Columbia University, New York, New Yorken_US
dc.contributor.affiliationotherDepartment of Neurological Surgery, College of Physicians & Surgeons, Columbia University, New York, New Yorken_US
dc.contributor.affiliationotherDepartment of Neurological Surgery, College of Physicians & Surgeons, Columbia University, New York, New Yorken_US
dc.contributor.affiliationotherDepartment of Neurological Surgery, College of Physicians & Surgeons, Columbia University, New York, New Yorken_US
dc.contributor.affiliationotherDepartment of Neurological Surgery, College of Physicians & Surgeons, Columbia University, New York, New Yorken_US
dc.contributor.affiliationotherDepartment of Pediatrics, College of Physicians & Surgeons, Columbia University, New York, New Yorken_US
dc.contributor.affiliationotherDepartment of Neurological Surgery, College of Physicians & Surgeons, Columbia University, New York, New Yorken_US
dc.identifier.pmid16447284en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/50651/1/20775_ftp.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1002/jnr.20775en_US
dc.identifier.sourceJournal of Neuroscience Researchen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


Files in this item

Show simple item record

Accessibility: If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.