Temporal pattern of C1q deposition after transient focal cerebral ischemia

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dc.contributor.author Mack, William J. en_US
dc.contributor.author Sughrue, Michael E. en_US
dc.contributor.author Ducruet, Andrew F. en_US
dc.contributor.author Mocco, J. en_US
dc.contributor.author Sosunov, Sergey A. en_US
dc.contributor.author Hassid, Benjamin G. en_US
dc.contributor.author Silverberg, Joshua Z. en_US
dc.contributor.author Ten, Vadim S. en_US
dc.contributor.author Pinsky, David J. en_US
dc.contributor.author Connolly, E. Sander en_US
dc.date.accessioned 2007-05-02T14:16:17Z
dc.date.available 2007-05-02T14:16:17Z
dc.date.issued 2006-04 en_US
dc.identifier.citation Mack, William J.; Sughrue, Michael E.; Ducruet, Andrew F.; Mocco, J; Sosunov, Sergey A.; Hassid, Benjamin G.; Silverberg, Joshua Z.; Ten, Vadim S.; Pinsky, David J.; Connolly, E. Sander (2006). "Temporal pattern of C1q deposition after transient focal cerebral ischemia." Journal of Neuroscience Research 83(5): 883-889. <http://hdl.handle.net/2027.42/50651> en_US
dc.identifier.issn 0360-4012 en_US
dc.identifier.issn 1097-4547 en_US
dc.identifier.uri http://hdl.handle.net/2027.42/50651
dc.identifier.uri http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=16447284&dopt=citation en_US
dc.description.abstract Recent studies have focused on elucidating the contribution of individual complement proteins to post-ischemic cellular injury. As the timing of complement activation and deposition after cerebral ischemia is not well understood, our study investigates the temporal pattern of C1q accumulation after experimental murine stroke. Brains were harvested from mice subjected to transient focal cerebral ischemia at 3, 6, 12, and 24 hr post reperfusion. Western blotting and light microscopy were employed to determine the temporal course of C1q protein accumulation and correlate this sequence with infarct evolution observed with TTC staining. Confocal microscopy was utilized to further characterize the cellular localization and characteristics of C1q deposition. Western Blot analysis showed that C1q protein begins to accumulate in the ischemic hemisphere between 3 and 6 hr post-ischemia. Light microscopy confirmed these findings, showing concurrent C1q protein staining of neurons. Confocal microscopy demonstrated co-localization of C1q protein with neuronal cell bodies as well as necrotic cellular debris. These experiments demonstrate the accumulation of C1q protein on neurons during the period of greatest infarct evolution. This data provides information regarding the optimal time window during which a potentially neuroprotective anti-C1q strategy is most likely to achieve therapeutic success. © 2006 Wiley-Liss, Inc. en_US
dc.format.extent 1237241 bytes
dc.format.extent 3118 bytes
dc.format.mimetype application/pdf
dc.format.mimetype text/plain
dc.publisher Wiley Subscription Services, Inc., A Wiley Company en_US
dc.subject.other Life and Medical Sciences en_US
dc.subject.other Neuroscience, Neurology and Psychiatry en_US
dc.title Temporal pattern of C1q deposition after transient focal cerebral ischemia en_US
dc.type Article en_US
dc.rights.robots IndexNoFollow en_US
dc.subject.hlbsecondlevel Molecular, Cellular and Developmental Biology en_US
dc.subject.hlbsecondlevel Neurosciences en_US
dc.subject.hlbsecondlevel Psychology en_US
dc.subject.hlbsecondlevel Public Health en_US
dc.subject.hlbtoplevel Health Sciences en_US
dc.subject.hlbtoplevel Science en_US
dc.subject.hlbtoplevel Social Sciences en_US
dc.description.peerreviewed Peer Reviewed en_US
dc.contributor.affiliationum Division of Cardiology, Department of Medicine, University of Michigan, Ann Arbor, Michigan en_US
dc.contributor.affiliationother Department of Neurological Surgery, College of Physicians & Surgeons, Columbia University, New York, New York ; Dept. of Neurological Surgery, Columbia University, 710 West 168th Street, New York, NY 10032 en_US
dc.contributor.affiliationother Department of Neurological Surgery, College of Physicians & Surgeons, Columbia University, New York, New York en_US
dc.contributor.affiliationother Department of Neurological Surgery, College of Physicians & Surgeons, Columbia University, New York, New York en_US
dc.contributor.affiliationother Department of Neurological Surgery, College of Physicians & Surgeons, Columbia University, New York, New York en_US
dc.contributor.affiliationother Department of Neurological Surgery, College of Physicians & Surgeons, Columbia University, New York, New York en_US
dc.contributor.affiliationother Department of Neurological Surgery, College of Physicians & Surgeons, Columbia University, New York, New York en_US
dc.contributor.affiliationother Department of Neurological Surgery, College of Physicians & Surgeons, Columbia University, New York, New York en_US
dc.contributor.affiliationother Department of Pediatrics, College of Physicians & Surgeons, Columbia University, New York, New York en_US
dc.contributor.affiliationother Department of Neurological Surgery, College of Physicians & Surgeons, Columbia University, New York, New York en_US
dc.identifier.pmid 16447284 en_US
dc.description.bitstreamurl http://deepblue.lib.umich.edu/bitstream/2027.42/50651/1/20775_ftp.pdf en_US
dc.identifier.doi http://dx.doi.org/10.1002/jnr.20775 en_US
dc.identifier.source Journal of Neuroscience Research en_US
dc.owningcollname Interdisciplinary and Peer-Reviewed
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