Now showing items 1-10 of 32
Cysteine 265 Is in the Active Site of, But Is Not Essential for Catalysis by tRNA-Guanine Transglycosylase (TGT) from Escherichia coli
(Kluwer Academic Publishers-Plenum Publishers; Plenum Publishing Corporation ; Springer Science+Business Media, 1997-01)
Site-directed mutagenesis and X-ray absorption spectroscopy studies have previously shown that the tRNA-guanine transglycosylase (TGT) from Escherichia coli is a zinc metalloprotein and identified the enzymic ligands to ...
Synthesis of normal and variant human hypoxanthine-guanine phosphoribosyltransferase in Escherichia coli
(Elsevier, 1993-01-30)
Naturally occurring mutations in hypoxanthine-guanine phosphoribosyltransferase (HPRT) have been identified by amino acid sequencing, cDNA cloning, and direct nucleotide sequencing of PCR-amplified transcripts. To determine ...
Significance of Root-Mean-Square Deviation in Comparing Three-dimensional Structures of Globular Proteins
(Elsevier, 1994-01-13)
In the study of globular protein conformations, one customarily measures the similarity in three-dimensional structure by the root-mean-square deviation (RMSD) of the C[alpha] atomic coordinates after optimal rigid body ...
Directed biosynthesis of unnatural alkaloids in Dolichothele sphaerica
(Elsevier, 1974-05)
Using appropriate precursors, the two unnatural alkaloids 4(5)-[N-isocaproylaminomethyl]imidazole and 3-[2-N-isovalerylaminoethyl]pyrazole were produced by Dolichothele sphaerica. The former compound represents an unnatural ...
Antinociceptive effects of [D-Ala2]deltorphin II, a highly selective [delta] agonist in vivo
(Elsevier, 1990)
The present study has characterized the antinociceptive actions of [D-Ala2]deltorphin II following intracerebroventricular (i.c.v.) administration in the mouse tail-flick test. [D-Ala2]deltorphin II produced dose- and ...
CASA: An Efficient Automated Assignment of Protein Mainchain NMR Data Using an Ordered Tree Search Algorithm
(Kluwer Academic Publishers; Springer, 2005-12)
Rapid analysis of protein structure, interaction, and dynamics requires fast and automated assignments of 3D protein backbone triple-resonance NMR spectra. We introduce a new depth-first ordered tree search method of ...
The isolation and synthesis of dolichotheline
(Elsevier, 1970-03)
An imidazole alkaloid named dolichotheline has been isolated from Dolichothele sphaerica (Dietrich) Britton and Rose, and characterized as 4(5)-[2-N-isovalerylaminoethyl[imidazole (N-isovalerylhistamine) (I). Its structure ...
Statistical mechanics of protein folding by exhaustive enumeration
(Wiley Subscription Services, Inc., A Wiley Company, 1998-09-01)
It is hard to construct theories for the folding of globular proteins because they are large and complicated molecules having enormous numbers of nonnative conformations and having native states that are complicated to ...
Failures of inverse folding and threading with gapped alignment
(Wiley Subscription Services, Inc., A Wiley Company, 1996-10)
To calculate the tertiary structure of a protein from its amino acid sequence, the thermodynamic approach requires a potential function of sequence and conformation that has its global minimum at the native conformation ...
Low molecular weight protamine as an efficient and nontoxic gene carrier: in vitro study
(John Wiley & Sons, Ltd., 2003-08)
Background The structural similarity between low molecular weight protamine (LMWP), prepared by enzymatic digestion of protamine, and HIV-TAT protein transduction peptide suggested the feasibility of LMWP as an efficient ...