Now showing items 11-20 of 32
Adenovirus binding to cultured synoviocytes triggers signaling through MAPK pathways and induces expression of cyclooxygenase-2
(John Wiley & Sons, Ltd., 2005-03)
Background Recombinant adenovirus can be administered in vivo to achieve transduction of a number of cell types including human synoviocytes. Immunogenicity of adenoviruses has limited their utility as vectors for gene ...
tRNA-Guanine Transglycosylase from Escherichia coli: Overexpression, Purification and Quaternary Structure
(Elsevier, 1993-05-20)
tRNA-guanine transglycosylase (TGT) is the enzyme responsible for the post-transcriptional modification of specific tRNAs (Asn, Asp, His and Tyr) with queuine. In E. coli this modification occurs via a two-step reaction: ...
Size-independent comparison of protein three-dimensional structures
(Wiley Subscription Services, Inc., A Wiley Company, 1995-07)
Protein structures are routinely compared by their root-mean-square deviation (RMSD) in atomic coordinates after optimal rigid body superposition. What is not so clear is the significance of different RMSD values, particularly ...
Stereochemical course of the biosynthesis of 1-aminocyclopropane-1-carboxylic acid. I. Role of the asymmetric sulfonium pole and the [alpha]-amino acid center
(Elsevier, 1984-05-31)
The substrate stereospecificity of 1-aminocyclopropane-1-carboxylic acid synthase, a pyridoxal phosphate-containing enzyme, from the pericarp tissue of (tomatoes) was studied using the various stereoisomers of -adenosylmethionine ...
A Comparative Study of Human Muscle and Brain Creatine Kinases Expressed in Escherichia coli
(Kluwer Academic Publishers-Plenum Publishers; Plenum Publishing Corporation ; Springer Science+Business Media, 2000-01)
We report the expression of the human muscle (CK-MM) and brain (CK-BB) creatine kinases in Escherichia coli . The proteins have been purified to apparent homogeneity and several of their physical and kinetic properties ...
Kinetic and Mutagenic Evidence for the Role of Histidine Residues in the Lycopersicon esculentum 1-Aminocyclopropane-1-carboxylic acid Oxidase
(Kluwer Academic Publishers-Plenum Publishers; Plenum Publishing Corporation ; Springer Science+Business Media, 1999-01)
The ACCO gene from Lycopersicon esculentum (tomato) has been cloned into the expression vector PT7-7. The highly expressed protein was recovered in the form of inclusion bodies. ACCO is inactivated by diethyl pyrocarbonate ...
Discriminative and aversive properties of [beta]-carboline-3-carboxylic acid ethyl ester, a benzodiazepine receptor inverse agonist, in rhesus monkey
(Elsevier, 1986-03-17)
Rhesus monkeys were trained to discriminate injections of saline from those of [beta]-carboline-3-carboxylic acid ethyl ester ([beta]-CCE), a compound that binds to the benzodiazepine receptor, but often has actions opposite ...
Biosynthesis of phenolic tetrahydroisoquinoline alkaloids of peyote
(Elsevier, 1970-08)
Data are presented supporting a proposed pathway of biosynthesis of the phenolic tetrahydroisoquinoline alkaloids of peyote from dopamine. It is suggested that meta-O-methylation of dopamine, hydroxylation in the 5-position, ...
A 13 base pair deletion in exon 1 of HPRT Illinois forms a functional GUG initiation codon
(Springer-Verlag, 1994-03)
More than 50 mutations in the human hypoxanthine-guanine phosphoribosyltransferase (HPRT) locus have been described, yet only 2 alter the AUG initiation codon. One, variant HPRT 1151 , results in Lesch-Nyhan syndrome (LNS), ...