Now showing items 1-10 of 10
Injectable microparticle-gel system for prolonged and localized lidocaine release. I. In vitro characterization
(Wiley Subscription Services, Inc., A Wiley Company, 2004-09-01)
Current treatment protocol for postoperative pain is to infuse anesthetic solution around nerves or into the epidural space. This clinical practice is beset by the short duration of the anesthetic effect unless the infusion ...
Heterologous expression of tylosin polyketide synthase and production of a hybrid bioactive macrolide in Streptomyces venezuelae
(Springer-Verlag, 2006-02-22)
Tylosin polyketide synthase (Tyl PKS) was heterologously expressed in an engineered strain of Streptomyces venezuelae bearing a deletion of pikromycin PKS gene cluster using two compatible low-copy plasmids, each under the ...
Poly(glutamic acid) poly(ethylene glycol) hydrogels prepared by photoinduced polymerization: Synthesis, characterization, and preliminary release studies of protein drugs
(Wiley Subscription Services, Inc., A Wiley Company, 2002-10)
A class of new biodegradable hydrogels based on poly(ethylene glycol) methacrylate-graft-poly(glutamic acid) and poly(ethylene glycol) dimethacrylate was synthesized by photoinduced polymerization. Because all the polymeric ...
Injectable microparticle–gel system for prolonged and localized lidocaine release. II. in vivo anesthetic effects
(Wiley Subscription Services, Inc., A Wiley Company, 2004-09-01)
Current treatment protocols for postoperative pain are beset by either the short duration of the anesthetic effect or requirement of hospitalization of the patients. We reported herein a novel treatment by applying to the ...
Low molecular weight protamine as an efficient and nontoxic gene carrier: in vitro study
(John Wiley & Sons, Ltd., 2003-08)
Background The structural similarity between low molecular weight protamine (LMWP), prepared by enzymatic digestion of protamine, and HIV-TAT protein transduction peptide suggested the feasibility of LMWP as an efficient ...
Adenovirus binding to cultured synoviocytes triggers signaling through MAPK pathways and induces expression of cyclooxygenase-2
(John Wiley & Sons, Ltd., 2005-03)
Background Recombinant adenovirus can be administered in vivo to achieve transduction of a number of cell types including human synoviocytes. Immunogenicity of adenoviruses has limited their utility as vectors for gene ...
Surface-modified poly(lactide- co -glycolide) nanospheres for targeted bone imaging with enhanced labeling and delivery of radioisotope
(Wiley Subscription Services, Inc., A Wiley Company, 2003-12-01)
Surface-modified nanospheres can be utilized for targeting drugs and diagnostic agents to the bone and bone marrow while extending their circulation time in the blood stream. The surface modification of poly(lactide- co ...
Restituting intestinal epithelial cells exhibit increased transducibility by adenoviral vectors
(John Wiley & Sons, Ltd., 2006-12)
Background and aims While mature enterocytes are resistant to transduction by adenovirus type 5 (Ad5) vectors, undifferentiated cells are transduced much more efficiently. Our purpose was to study enterocyte transduction ...
A Less Toxic Heparin Antagonist—Low Molecular Weight Protamine
(Kluwer Academic Publishers-Plenum Publishers; MAIK “Nauka/Interperiodica” ; Springer Science+Business Media, 2003-01)
A new thirteen amino acid peptide, named low molecular weight protamine (LMWP), was obtained through the enzymatic digestion of native protamine. Both in vitro and in vivo results showed that LMWP fully maintained the ...
Lipopolysaccharide recognition protein, MD-2, facilitates cellular uptake of E. coli -derived plasmid DNA in synovium
(John Wiley & Sons, Ltd., 2005-07)
Background Several cell types are susceptible to transfection in vivo using naked plasmid DNA. The mechanisms involved in mediating in vivo transfection are incompletely known, but evidence suggests that receptor-mediated ...