Centrosome misorientation reduces stem cell division during ageing
dc.contributor.author | Cheng, Jun | en_US |
dc.contributor.author | Turkel, Nezaket | en_US |
dc.contributor.author | Hemati, Nahid | en_US |
dc.contributor.author | Fuller, Margaret T. | en_US |
dc.contributor.author | Hunt, Alan J. | en_US |
dc.contributor.author | Yamashita, Yukiko M. | en_US |
dc.date.accessioned | 2009-06-01T17:42:30Z | |
dc.date.available | 2009-06-01T17:42:30Z | |
dc.date.issued | 2008-12-04 | en_US |
dc.identifier.citation | Cheng, Jun; Turkel, Nezaket; Hemati, Nahid; Fuller, Margaret T.; Hunt, Alan J.; Yamashita, Yukiko M.. (2008) "Centrosome misorientation reduces stem cell division during ageing." Nature 456(7222): 599-U40. <http://hdl.handle.net/2027.42/62879> | en_US |
dc.identifier.issn | 0028-0836 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/62879 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=18923395&dopt=citation | en_US |
dc.description.abstract | Asymmetric division of adult stem cells generates one self- renewing stem cell and one differentiating cell, thereby maintaining tissue homeostasis. A decline in stem cell function has been proposed to contribute to tissue ageing, although the underlying mechanism is poorly understood. Here we show that changes in the stem cell orientation with respect to the niche during ageing contribute to the decline in spermatogenesis in the male germ line of Drosophila. Throughout the cell cycle, centrosomes in germline stem cells ( GSCs) are oriented within their niche and this ensures asymmetric division. We found that GSCs containing misoriented centrosomes accumulate with age and that these GSCs are arrested or delayed in the cell cycle. The cell cycle arrest is transient, and GSCs appear to re- enter the cell cycle on correction of centrosome orientation. On the basis of these findings, we propose that cell cycle arrest associated with centrosome misorientation functions as a mechanism to ensure asymmetric stem cell division, and that the inability of stem cells to maintain correct orientation during ageing contributes to the decline in spermatogenesis. We also show that some of the misoriented GSCs probably originate from dedifferentiation of spermatogonia. | en_US |
dc.description.sponsorship | University of Michigan ; March of Dimes Basil O'Conner Starter Scholar Research Award ; Searle Scholar Program ; NIH [P01 DK53074, R01GM072006] | en_US |
dc.description.sponsorship | We thank C. Gonzalez, D. McKearin, N. Rusan, M. Peifer and the Bloomington Stock Center for fly stocks; R. Lehmann, C. Field and the Developmental Studies Hybridoma Bank for antibodies; M. Kiel and D. Nakada for help with X-ray irradiation; and S. Morrison and T. Mahowald for comments on the manuscript. This research was supported by a University of Michigan start-up fund, March of Dimes Basil O'Conner Starter Scholar Research Award and the Searle Scholar Program (to Y.M.Y.), and NIH grants P01 DK53074 (to M.T.F.) and R01GM072006 (to A.J.H.). | en_US |
dc.format.extent | 1103395 bytes | |
dc.format.extent | 2489 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Nature Publishing Group | en_US |
dc.source | Nature | en_US |
dc.title | Centrosome misorientation reduces stem cell division during ageing | en_US |
dc.type | Article | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Yamashita, Yukiko M.] Univ Michigan, Inst Life Sci, Ctr Stem Cell Biol, Ann Arbor, MI 48109 USA | en_US |
dc.contributor.affiliationum | Hunt, Alan J.] Univ Michigan, Dept Biomed Engn, Ctr Ultrafast Opt Sci, Ann Arbor, MI 48109 USA | en_US |
dc.contributor.affiliationum | [Yamashita, Yukiko M.] Univ Michigan, Dept Cell & Dev Biol, Ann Arbor, MI 48109 USA | en_US |
dc.contributor.affiliationother | [Turkel, Nezaket | en_US |
dc.contributor.affiliationother | Hemati, Nahid | en_US |
dc.contributor.affiliationother | [Cheng, Jun | en_US |
dc.contributor.affiliationother | [Fuller, Margaret T.] Stanford Univ, Sch Med, Dept Dev Biol, Stanford, CA 94305 USA | en_US |
dc.contributor.affiliationother | [Fuller, Margaret T.] Stanford Univ, Sch Med, Dept Genet, Stanford, CA 94305 USA | en_US |
dc.identifier.pmid | 18923395 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/62879/1/nature07386.pdf | |
dc.identifier.doi | http://dx.doi.org/10.1038/nature07386 | en_US |
dc.identifier.source | Nature | en_US |
dc.contributor.authoremail | yukikomy@umich.edu | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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