Functional Analysis of MycE and MycF, Two O -Methyltransferases Involved in the Biosynthesis of Mycinamicin Macrolide Antibiotics
dc.contributor.author | Li, Shengying | en_US |
dc.contributor.author | Anzai, Yojiro | en_US |
dc.contributor.author | Kinoshita, Kenji | en_US |
dc.contributor.author | Kato, Fumio | en_US |
dc.contributor.author | Sherman, David H. | en_US |
dc.date.accessioned | 2009-06-01T19:16:46Z | |
dc.date.available | 2010-06-02T14:34:28Z | en_US |
dc.date.issued | 2009-05-25 | en_US |
dc.identifier.citation | Li, Shengying; Anzai, Yojiro; Kinoshita, Kenji; Kato, Fumio; Sherman, David H. (2009). "Functional Analysis of MycE and MycF, Two O -Methyltransferases Involved in the Biosynthesis of Mycinamicin Macrolide Antibiotics." ChemBioChem 10(8): 1297-1301. <http://hdl.handle.net/2027.42/62994> | en_US |
dc.identifier.issn | 1439-4227 | en_US |
dc.identifier.issn | 1439-7633 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/62994 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=19415708&dopt=citation | en_US |
dc.description.abstract | Mg motors : We characterized the in vitro function of MycE and MycF, two O -methyltransferases involved in the biosynthesis of mycinamicin antibiotics. Each enzyme was confirmed to be an S -adenosyl- L -methionine (SAM)-dependent deoxysugar methyltransferase. Their optimal activities require the presence of Mg 2+ . With the reconstituted in vitro assays, the order of mycinamicin VI→III→IV in the post-PKS (polyketide synthase) tailoring pathway of mycinamicin was unambiguously determined. | en_US |
dc.format.extent | 439830 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | WILEY-VCH Verlag | en_US |
dc.subject.other | Chemistry | en_US |
dc.subject.other | Biochemistry and Biotechnology | en_US |
dc.title | Functional Analysis of MycE and MycF, Two O -Methyltransferases Involved in the Biosynthesis of Mycinamicin Macrolide Antibiotics | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Life Sciences Institute, Department of Medicinal Chemistry, Chemistry, and Microbiology and Immunology, University of Michigan, 210 Washtenaw Avenue, Ann Arbor, MI 48109-2216 (USA), Fax: (+1) 734-615-3641 | en_US |
dc.contributor.affiliationum | Life Sciences Institute, Department of Medicinal Chemistry, Chemistry, and Microbiology and Immunology, University of Michigan, 210 Washtenaw Avenue, Ann Arbor, MI 48109-2216 (USA), Fax: (+1) 734-615-3641 ; Faculty of Pharmaceutical Sciences, Toho University, 2-2-1 Miyama, Funabashi, Chiba 274-8510 (Japan) | en_US |
dc.contributor.affiliationum | Life Sciences Institute, Department of Medicinal Chemistry, Chemistry, and Microbiology and Immunology, University of Michigan, 210 Washtenaw Avenue, Ann Arbor, MI 48109-2216 (USA), Fax: (+1) 734-615-3641 | en_US |
dc.contributor.affiliationother | School of Pharmaceutical Sciences, Mukogawa Women's University, 11-68 Kyuban-cho, Koshien, Nishinomiya 663-8179 (Japan) | en_US |
dc.contributor.affiliationother | Faculty of Pharmaceutical Sciences, Toho University, 2-2-1 Miyama, Funabashi, Chiba 274-8510 (Japan) | en_US |
dc.identifier.pmid | 19415708 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/62994/1/1297_ftp.pdf | |
dc.identifier.doi | 10.1002/cbic.200900088 | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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