Now showing items 41-50 of 132
Quantitation of oligosaccharides released by the [beta]-elimination reaction
(Elsevier, 1980-01-01)
A quantitative micromethod has been described for monitoring the rate and extent of the [beta]-elimination reaction as applied to O-glycosyl-glycoproteins utilizing alkaline tritiated borohydride. The procedure simultaneously ...
Immobilization and assay of low-molecular-weight phosphomannosyl receptor in multiwell plates
(Elsevier, 1987-10)
A novel sensitive binding assay for quantitation of a low-molecular-weight phosphomannosyl receptor (41-46 K) was devised. The receptor was immobilized by immunochemical means in the wells of polystyrene multiwell plates. ...
S18.20 Preparation of two kinds of sialic acid specific monomeric monovalent lectin derivatives and their application to the study of cell surface glycoconjugates by flow cytometry
(Kluwer Academic Publishers; Chapman & Hall ; Springer Science+Business Media, 1993-08)
Isolation of a novel plant lectin with an unusual specificity from Calystegia sepium
(Kluwer Academic Publishers; Chapman and Hall ; Springer Science+Business Media, 1997-01)
A novel plant lectin has been isolated from the rhizomes of Calystegia sepium (hedge bindweed) and partially characterized. The lectin is a dimeric protein composed of two identical non-covalently linked subunits of 16kDa. ...
The interaction of nitrous oxide with cobalamin-dependent methionine synthase
(Elsevier, 1991-08)
Bovine testicular [beta]-galactosidase: Purification of enzyme fractions that exhibit high affinity for phosphomannosyl receptors
(Elsevier, 1989-11-01)
An improved method is described for the preparation of bovine testicular [beta]-galactosidase that allows the isolation of enzyme fractions that bind avidly to phosphomannosyl receptors. The procedure permits removal of a ...
An inexpensive, microcomputer-based, video densitometer for quantitating thin-layer chromatographic spots
(Elsevier, 1983-07-01)
An inexpensive video densitometer based on an Apple II microcomputer using commercially available components was developed. The device was used to quantitate charred lipid and colored spots on thin-layer chromatography ...
Subcellular distribution of terminal α-D- and β-D-galactosyl residues in Ehrlich tumour cells studied by lectin-gold techniques
(Kluwer Academic Publishers; Chapman & Hall ; Springer Science+Business Media, 1995-04)
We have studied by high resolution in situ light and electron microscopic lectin-gold techniques the subcellular distribution of α- d -Gal residues using the Griffonia simplicifolia I-B 4 isolectin and compared it with ...
Subcellular distribution and properties of acyl/alkyl dihydroxyacetone phosphate reductase in rodent livers
(Elsevier, 1986-03)
On subcellular fractionation, the enzyme acyl/alkyl dihydroxyacetone phosphate (DHAP) reductase (EC 1.1.1.101) in guinea pig and rat liver was found to be present in both the light mitochondrial (L) and microsomal fractions. ...