Now showing items 21-30 of 132
Simple, rapid, and highly efficient separation of amino acid phenylthiohydantoins by reversed-phase high-performance liquid chromatography
(Elsevier, 1982-04)
A rapid and efficient separation of amino acid phenylthiohydantoins by high-performance liquid chromatography has been accomplished by step-gradient elution with use of a mobile phase of acetate-buffered aqueous acetonitrile ...
Quantitative determination of reducing sugars, oligosaccharides, and glycoproteins with [3H]borohydride
(Elsevier, 1973-09)
A very simple and direct radioactive isotope method has been described for the quantitative determination of reducing sugars. The method can be adapted to give any degree of sensitivity desired. The procedure is of general ...
Initiation of poly-N-acetyllactosamine chain biosynthesis occurs preferentially on complex multiantennary asparagine-linked oligosaccharides
(Elsevier, 1990-08-01)
An N-acetyl-[beta]--glucosaminyltransferase activity involved in the initiation of poly-N-acetyllactosamine chain biosynthesis can be solubilized from Ehrlich ascites tumor cell microsomal membranes. The ability of this ...
Binding of hydrophobic ligands to plant lectins: Titration with arylaminonaphthalenesulfonates
(Elsevier, 1983-07-15)
Binding of the Hydrophobic ligands 1,8-anilinonaphthalenesulfonic acid (ANS) and 2,6-toluidinylnaphthalenesulfonic acid (TNS) to a variety of plant lectins was studied by lectin-induced alteration of the fluorescence spectra ...
Isolation and characterization of a glycopeptide from human senescent erythrocytes
(Elsevier, 1983-08-16)
A glycopeptide (called "senescence-factor glycopeptide", SF-G) has been isolated from a tryptic digest of human erythrocytes by specific adsorption and elution from immobilized peanut lectin. SF-G was detectable in old but ...
Synthesis of high-affinity, hydrophobic monosaccharide derivatives and study of their interaction with concanavalin A, the pea, the lentil, and fava bean lectins
(Elsevier, 1992-12)
Concanavalin A (Con A) and agglutinins from the pea (PSA), lentil (LCH), and fava bean (VFA) constitute a group of -mannose/-glucose binding legume lectins. In addition to their sugar binding specificity, these lectins ...
The purification and properties of an aryl [beta]-hexosidase from bovine liver
(Elsevier, 1977-01-30)
An aryl [beta]-hexosidase was purified 800-fold from bovine liver. The purified enzyme hydrolyzed p-nitrophenyl glycosylpyranoside derivatives of [beta]--galactose, [beta]--glucose, [beta]--xylose, [beta]--mannose, and ...
Protein---carbohydrate interaction : Part XXI. The interaction of concanavalin a with d-fructans
(Elsevier, 1969-06)
The immunochemical techniques of quantitative precipitation and hapten inhibition have been employed in a study of the interaction of concanavalin A with several levans of bacterial and plant origin. Of the polysaccharides ...
Role of alcohol P-450-oxygenase (APO) in microsomal ethanol oxidation
(Elsevier, 1985)
The form of liver microsomal cytochrome P-450 induced by chronic administration of ethanol to rabbits, designated as P-450 or P-450 isozyme 3a, has been purified to homogeneity as judged by several criteria, including NH2- ...