Now showing items 51-60 of 132
A sensitive fluorescent method for the detection of glycoproteins in polyacrylamide gels
(Elsevier, 1976-05-21)
A sensitive method is described for the detection of glycoproteins after polyacrylamide gel electrophoresis. Dansyl hydrazine is condensed with aldehydic functions generated by periodate oxidation of protein bound carbohydrate. ...
Radiometric assay for cytochrome P-450-catalyzed progesterone 16[alpha]-hydroxylation and determination of an apparent isotope effect
(Elsevier, 1987-08-01)
In the course of studies on the oxygenation of steroids by purified P-450 cytochromes, particularly rabbit liver microsomal cytochrome P-450 form 3b, a rapid and reliable radiometric assay has been devised for progesterone ...
Properties of the enzymes catalyzing the biosynthesis of lysophosphatidate and its ether analog in cultured fibroblasts from Zellweger syndrome patients and normal controls
(Elsevier, 1987-05-01)
The activities, properties, and steady-state kinetics of the five enzymes catalyzing the synthesis of 1-acyl- and 1-alkyl-sn-glycerol 3-phosphate in the cultured skin fibroblasts from Zellweger syndrome patients and normal ...
Characterization of gangliosides from Ehrlich ascites tumour cells and their variants
(Kluwer Academic Publishers; Chapman & Hall ; Springer Science+Business Media, 1996-04)
Differences in the nature of the gangliosides present in two types of Ehrlich ascites tumour (EAT) cells, the adherent and non-adherent EAT cells, were studied. Gangliosides were isolated by DEAE Sephadex column chromatography ...
Gas-liquid chromatography of carbohydrate derivatives: the separation and quantitative determination of synthetic and naturally occurring glycosyl derivatives of amino acids
(Elsevier, 1972-05)
A gas-liquid chromatographic separation of the trimethylsilyl derivatives of several synthetic and naturally occurring glycosyl derivatives of amino acids is described. This technique may be used for the qualitative and ...
Radioiodination of sulfhydryl-sensitive proteins
(Elsevier, 1975-08)
A new procedure is described for the radioiodination of proteins with sulfhydryl groups essential for their biological activity. Aniline is iodinated with 125I-labeled sodium iodide in the presence of chloramine-T, the ...
Properties of the lectin from the hog peanut (Amphicarpaea bracteata)
(Elsevier, 1987-05-15)
An N-acetyl--galactosamine-specific lectin has been isolated from the two seed forms of the hog peanut (Amphicarpaea bracteata) using an affinity support containing the synthetic type A blood group trisaccharide ...
Preparation of cyrstalline [alpha]-kojibiose octaacetate from dextran B-1299-S: Its conversion into p-nitrophenyl and p-isothiocyanatophenyl [beta]-kojibioside
(Elsevier, 1973-03)
Acetolysis of dextran B-1299-S provides a preparative approach to crystalline [alpha]-kojibiose octaacetate. The latter was transformed via crystalline hepta-O-acetyl-[alpha]-kojibiosyl bromide into p-nitrophenyl and ...
A microscale isolation of hemins from hemeproteins by use of polyacrylamide gel electrophoresis
(Elsevier, 1975)
An analytical and semipreparative procedure has been developed for the isolation of the prosthetic groups of various hemeproteins by use of polyacrylamide gel electrophoresis. Electrophoresis of erythrocyte formylhemeprotein ...
Properties of NADPH-cytochrome P-450 reductase purified from rabbit liver microsomes
(Elsevier, 1979-07)
NADPH-cytochrome P-450 reductase has been purified to electrophoretic homogeneity from rabbit liver microsomes by a procedure that may be used in conjunction with the isolation of the major forms of cytochrome P-450. The ...