Now showing items 1-10 of 106
Immunochemical studies on the metabolism of nitrosamines by ethanol-inducible cytochrome P-450
(Elsevier, 1985-04-30)
The ethanol-induced rabbit liver microsomal cytochrome P-450, P-450LM3a, has been shown previously to efficiently catalyze the demethylation of N-nitrosodimethylamine (NDMA) with a Km of 2.9 mM. Since the predominant Km ...
Datura stramonium lectin: isolation and characterization of the homogeneous lectin
(Elsevier, 1981-07-20)
Renewed interest in the polyphosphoinositides
(Elsevier, 1984-02)
The significance of the enhanced cellular phosphatidate and phosphatidylinositol turnover which occurs in response to specific extracellular messengers has been the subject of much interest and speculation. Until recently, ...
Biosynthesis of alkyl-ether containing lipid from dihydroxyacetone phosphate
(Elsevier, 1969-10-22)
A lipid, stable to alkaline methanolysis, is formed when liver mitochondria or brain microsomes are incubated with dihydroxyacetone phosphate, long chain alcohol, ATP, CoA, NaF and Mg++. The lipid has been identified as ...
Acetylcholine stimulates hydrolysis of 32P-labeled phosphatidic acid in guinea pig synaptosomes
(Elsevier, 1973-02-05)
[3H]-inositol or [3H]-arachidonate was injected intracerebrally into guinea pigs. Labeled nerve endings were incubated with Ach* or CCh, both of which stimulate labeling of PhA and PhI from 32Pi by > 100% and 70% ...
Detection of intermediate protein conformations by room temperature tryptophan phosphorescence spectroscopy during denaturation of Escherichia coli alkaline phosphatase
(Elsevier, 1993-12)
The reversible denaturation of Escherichia coli alkaline phosphatase (AP) was followed by monitoring changes in enzymatic activity as well as by measurements of the time-resolved room temperature phosphorescence from Trp ...
Coupling of dihydroriboflavin oxidation to the formation of the higher valence states of hemeproteins
(Elsevier, 1991-11-27)
SummaryThe reactions between hydrogen peroxide and hemeproteins have been coupled to the oxidation of dihydroriboflavin so as to provide a simple method for measuring the rate constant of hemeprotein peroxidation. ...
Properties of the tryptophan residue in rabbit liver microsomal cytochrome P-450 isozyme 2 as determined by fluorescence
(Elsevier, 1985-04-30)
Cytochrome P-450 isozyme 2 from rabbit liver microsomes fluoresces upon excitation at 295 nm due to the single tryptophyl residue (Trp121) in the protein. The fluorescence spectrum, which is not altered by the presence of ...
Enzyme stereoselectivity: The reversible reaction catalyzed by 2-keto-4-hydroxyglutarate aldolase of Escherichia coli
(Elsevier, 1975-08-04)
SummaryThe condensation of [3-3H3]-pyruvate with glyoxylate in 3HOH to form [3-3H2]-ketohydroxyglutarate was catalyzed by ketohydroxyglutarate aldolase of Escherichia coli to study the stereochemical behavior of the enzyme. ...