Now showing items 1-8 of 8
GTP[gamma]S-stimulated hydrolysis of phosphatidyinositol-4,5-bisphosphate by soluble phospholipase C from human platelets requires soluble GTP-binding protein
(Elsevier, 1988-07-15)
GTP-binding activity was fractionated into two peaks (GI and GII) by chromatography on heparin-agarose. GTP-dependent PLC activity eluted as a single peak, which co-chromatographed with GTP-binding peak GII. Rechromatography ...
Differential activation of human skin cells by platelet activating factor: Stimulation of phosphoinositide turnover and arachidonic acid mobilization in keratinocytes but not in fibroblasts
(Elsevier, 1989-09-29)
Treatment of cultured adult human keratinocytes with platelet activating factor (PAF) resulted in a rapid, dose dependent accumulation of inositol phosphates. Inositol trisphosphate (IP3), inositol bisphosphate (IP2) and ...
Retinoic acid and synthetic analogs differentially activate retinoic acid receptor dependent transcription
(Elsevier, 1990-11-30)
SummaryWe have developed an assay where the potency of retinoids in retinoic acid receptor (RAR) mediated transcriptional activation can be rapidly evaluated. In this assay hRAR-[alpha], hRAR-[beta] and hRAR-[gamma] were ...
A retinoic acid-inducible skin-specific gene (RIS-1/psoriasin): molecular cloning and analysis of gene expression in human skin in vivo and cultured skin cells in vitro
(Kluwer Academic Publishers; Springer Science+Business Media, 1994-06)
A retinoic acid (RA) inducible skin-specific gene transcript (RIS-1) was isolated by differential hybridization screening of a RA-treated human skin cDNA library. The library was constructed from pooled RNA derived from ...
GTP and cytosol stimulate phosphoinositide hydrolysis in isolated platelet membranes
(Elsevier, 1986-06-13)
Hydrolysis of polyphosphoinositides by phospholipase C was examined in isolated membranes prepared from [32P]labelled platelets. In the presence of GTP[gamma]S, thrombin increased the release of inositol triphosphate and ...
Insertional mutation of the hairless locus on mouse Chromosome 14
(Springer-Verlag, 1993-11)
Crosses between heterozygous transgenic mice from line 5053 produced offspring with progressive irreversible hair loss beginning at day 10. With increasing age, the skin of these animals became thicker and plicated in ...
Increased membrane-associated transglutaminase activity in psoriasis
(Elsevier, 1989-10-16)
Terminal differentiation of human skin involves the formation of an insoluble cross-linked protein envelope (CLE), which functions as an external barrier. To characterize terminal differentiation in the skin disease ...
Increased cyclic GMP and decreased cyclic AMP levels in the hyperplastic, abnormally differentiated epidermis of psoriasis
(Elsevier, 1973-09-16)
The genetic skin disease psoriasis has been examined as a model system that may provide an understanding of the control of normal epidermal specialization (differentiation) and the perturbed regulatory processes in ...