Appendix1: Differential expression data for zebrafish regeneration and mouse degeneration models.
Appendix2: Gene ontology data for zebrafish regeneration and mouse degeneration models.
Appendix3: Pathway data for zebrafish regeneration and mouse degeneration models.
Appendix4: Differential expression data and genes within linked peaks for mi2004 mutants.
Appendix5: Gene ontology data for mi2004 mutants.
Appendix6: Pathway data for mi2004 mutants.
Appendix7: Linkage plots for mi2004 mutants.
Appendix8: Inverse PCR and genome-walking data.
Sifuentes, C. J. (2016). Regulation of Müller glial stem cell properties: Insights from a zebrafish model (Doctoral dissertation). Retrieved from http://hdl.handle.net/2027.42/135939
This collection was produced as part of the project, “A ‘Big Data’ Approach to Understanding Neighborhood Effects in Chronic Illness Disparities.” The Investigators for the project are Tiffany Veinot, Veronica Berrocal, Phillipa Clarke, Robert Goodspeed, Daniel Romero, and VG Vinod Vydiswaran from the University of Michigan. The study took place from 2015-2016, with funding from the University of Michigan’s Social Sciences Annual Institute, MCubed, and the Sloan and Moore Foundations.
Contact: Tiffany Veinot, MLS, PhD
Office: 3443 North Quad
Phone: 734/615-8281
Email: tveinot@umich.edu
MCubed project page:
https://mcubed.umich.edu/projects/%E2%80%9Cbig-data%E2%80%9D-approach-understanding-neighborhood-effects-chronic-illness-disparities
This Collection is a compilation of data measured in the TCC engine at the University of Michigan, Department of Mechanical Engineering, Quantitative Laser Diagnostics Laboratory. The posted Work Deposits are never changed. However, this collection will be expanded with additional Work Deposits as new experimental data become available. The intent of the collection is to provide a comprehensive experimental data set from the TCC-III engine, for fundamental discovery research on in-cylinder flow and spark-ignited combustion. Also, to enable in-depth support for CFD development and validation. The collection includes data files of in-cylinder flowvelocity and flame imaging, as well as engine and system geometry needed to set up 1-D and CFD simulations. and README for TCCIII_Collection: https://umich.box.com/v/Collection-README-rev20180202
This is a collection of photos of villages located primarily in Central Mali. These photos are primarily of Dogon villages, but there are village photos of other nearby ethnicities, including Bangande, Fulbe, Tuareg, Songay, and Bozo.These photos were taken to document the villages Professor Jeffrey Heath worked in and people he worked with while documenting languages throughout the region. For interactive geographical maps involving these villages see: http://dogonlanguages.org/geography.cfm.
Short documentary videos of practical activities and cultural events of Dogon, Fulbe, Songhay, and Bangande ethnic groups of eastern Central Mali. The videos were byproducts of linguistic research on the local language. They are presented here in three formats: wmv, avi, and either qt or mov. See the "readme" files in each work for a summary of the videos in it. and The footage was shot with various digital cameras. The oldest videos (2010 and one or two from 2011) were edited using iMovie. The later videos were edited using AVS editing software. Several of the 2010 videos, referred to as "compilations," are simple sequences of short clips that combine to illustrate a complex activity such as extracting oil from nuts. The later videos are in more flowing documentary form with overlaid titles in English. In some cases, vocabulary from the relevant native language is included in the titles.
This collection represents various raw data and analysis of cores extracted during the January 2009 mission of the research vessel Sproul in the Santa Barbara Basin., Cores included: box core SPR0901-04BC, box core SPR0901-unnamed, and Kasten core SPR0901-03KC. Core photos, physical properties and magnetic susceptibility from the multisensor track (MST), and the scanning X-ray fluorescence (XRF) data are included in the collection., and Cruise DOI: 10.7284/901089
This research is funded by NSF-OCE 0752093.
This collection represents various raw data and analysis of cores extracted during the November 2008 mission of R/V Melville in the Santa Barbara Basin., The core included is the jumbo piston core MV0811-14JC. Core photos, physical properties and magnetic susceptibility from the multisensor track (MST), and the scanning X-ray fluorescence (XRF) data are included in the collection., and Cruise DOI: 10.7284/903459
The research is funded by NSF OCE-1304327.
Craniosynostosis is the premature fusion of cranial bones. The goal of this study was to determine if delivery of recombinant tissue nonspecific alkaline phosphatase (TNAP) could prevent or diminish the severity of craniosynostosis in a C57BL/6 FGFR2C342Y/+ model of neonatal onset craniosynostosis or a BALB/c FGFR2C342Y/+ model of postnatal onset craniosynostosis. Mice were injected with a lentivirus encoding a mineral targeted form of TNAP immediately after birth. Cranial bone fusion as well as cranial bone volume, mineral content and density were assessed by micro computed tomography. Craniofacial shape was measured with calipers., Alkaline phosphatase, alanine amino transferase (ALT) and aspartate amino transferase (AST) activity levels were measured in serum. Neonatal delivery of TNAP diminished craniosynostosis severity from 94% suture obliteration in vehicle treated mice to 67% suture obliteration in treated mice, p<0.02) and the incidence of malocclusion from 82.4% to 34.7% (p<0.03), with no effect on cranial bone in C57BL/6 FGFR2C342Y/+ mice. In contrast, treatment with TNAP improved cranial bone volume (p< 0.01), density (p< 0.01) and mineral content (p< 0.01) but had no effect on craniosynostosis or malocclusion in BALB/c FGFR2C342Y/+ mice. , These results indicate that post-natal recombinant TNAP enzyme therapy diminishes craniosynostosis severity in the C57BL/6 FGFR2C342Y/+ neonatal onset mouse model of Crouzon syndrome, and that effects of exogenous TNAP are genetic background dependent., and Included in this collection is one set of images representing the C57BL/6 FGFR2C342Y/+ model of neonatal onset craniosynostosis, and one for the BALB/c FGFR2C342Y/+ model of postnatal onset craniosynostosis
The University of Michigan Museum of Zoology (UMMZ) is the center for the study of animal diversity on campus, focusing on the evolutionary origins of the planet’s animal species, the genetic information they contain and the communities and ecosystems they help form. Now an integral part of the Department of Ecology and Evolutionary Biology (EEB), the UMMZ houses world-class collections, containing more than 15 million specimens, span almost 200 years of regional and global biodiversity studies and that support a multi-faceted Departmental research and teaching program.
The Division of Mammals at the Museum of Zoology was established in 1837, and has grown steadily to its current size, with over 150,000 specimens. An important feature of the mammal collection at the Museum of Zoology is our emphasis on non-traditional specimens.
The Division of Reptiles and Amphibians maintains a collection that is worldwide in scope. The research collections contain over 200,000 catalogued lots representing approximately 435,000 individual specimens.
This collection contains estimates of the water balance of the Laurentian Great Lakes that were produced by the Large Lakes Statistical Water Balance Model (L2SWBM). Each data set has a different configuration and was used as the supplementary for a published peer-reviewed article (see "Citations to related material" section in the metadata of individual data sets). The key variables that were estimated by the L2SWBM are (1) over-lake precipitation, (2) over-lake evaporation, (3) lateral runoff, (4) connecting-channel outflows, (5) diversions, and (6) predictive changes in lake storage. and Contact: Andrew Gronewold
Office: 4040 Dana
Phone: (734) 764-6286
Email: drewgron@umich.edu
Smith, J. P., & Gronewold, A. D. (2017). Development and analysis of a Bayesian water balance model for large lake systems. arXiv preprint arXiv:1710.10161., Gronewold, A. D., Smith, J. P., Read, L., & Crooks, J. L. (2020). Reconciling the water balance of large lake systems. Advances in Water Resources, 103505., and Do, H.X., Smith, J., Fry, L.M., and Gronewold, A.D., Seventy-year long record of monthly water balance estimates for Earth’s largest lake system (under revision)
This data was produced by the site-based archaeological survey at the nuraghe S'Urachi in west-central Sardinia (San Vero Milis, Oristano, Sardinia). The survey was carried out from 2015-2017 as a part of the ongoing Progetto S'Urachi, an archaeological project that aims to understand daily life around the monumental Bronze Age tower of S'Urachi during the later occupation of the landscape over the course of the 1st millennium BCE.
Interest in quantitative imaging of Y-90 and Lu-177 is growing due to their increased use as minimally invasive treatments for primary and metastatic tumors such as HCC and NETs. Accurate quantification of the 3D activity distribution for voxel-level dosimetry requires SPECT/CT and PET/CT imaging. This collection provides research access to anonymized PET/CT and SPECT/CT scans along with the relevant lesion/organ contours taken from University of Michigan clinical research studies of selected patients undergoing radionuclide treatments. All patients signed an informed consent to participate in the research studies. See the readme in each dataset for information on use and citation of this data.
The data presented here were collected in the course of an archaeological survey of the region around Vani in the Republic of Georgia, carried out between 2009 and 2011, with follow-up visits in 2014 and 2017. The survey was sponsored by the University of Michigan, the Institute for the Study of the Ancient World at New York University, and the Otar Lordkipanidze Institute of Archaeological Research in Tbilisi. , The archaeological site of Vani lies in the territory of ancient Colchis, a triangular area bordered by the Black Sea to the west and by the greater and lesser Caucasus Mountains to the north and south – famous in Greek legend as the land of the Golden Fleece and the home of Medea. Vani itself is situated approximately 70 km inland, in the foothills of the lesser Caucasus. A regional culture recognizable on the evidence of distinctive traditions of pottery and metalworking and the appearance of a network of relatively large settlements had emerged in Colchis by the late second and early first millennia BC. , Vani is one of the most extensively studied archaeological sites in Colchis. Excavations have revealed a continuous occupation sequence extending from the 8th to the 1st centuries B.C. Especially notable are the rich and unusual graves of the Classical period (6th-4th centuries), the monumental stone architecture of the Hellenistic period (3rd to 1st centuries), especially fortifications enclosing an area of approximately 6 ha, and the extensive evidence for interaction with the Mediterranean and Near Eastern worlds, including not only commercial and luxury imports (Greek transport amphorae, fine bronze and silver utensils from both the Greek world and Persia), but also local production of bronze sculpture and one long Greek bronze inscription., In spite of the richness of the site, however, important questions about its purpose and function over time remain unresolved. How extensive was the ancient settlement? Was Vani an isolated stronghold, a regional population center, a sanctuary, or a combination of two or all three? How does it compare with other sites in Colchis? In addition to ongoing research at Vani itself, regional survey provides an obvious approach to some of these questions. In previous years, examination of a number of outlying sites had already yielded remains extending in date from the Early Bronze Age to the mediaeval period., and The purpose of the regional survey project begun in 2009 was to integrate existing knowledge about Vani and environs into the kind of technological and conceptual framework characteristic of contemporary American survey archaeology. Of particular importance was the use of Geographical Information Systems (GIS) as an organizational and analytical tool, and of geophysical prospection both in the immediate environs of Vani and at regional sites. Like all regional surveys, our project recorded evidence of all periods, from prehistory to the present day, but we were particularly interested in questions having to do with the increasing social complexity of Colchis in the mid- and later 1st millennium BC – when Greek explorers began to establish colonies on the Black Sea coast of Georgia, and the Persian empire pushed up against the mountains of the Caucasus. What was the nature of Colchian society in this period? How was it affected by interaction with the larger Greek, Persian, and Pontic worlds? And how can regional survey at Vani and throughout Colchis help us to address these questions?
This collection contains a hierarchy of test problems for turbulent reacting flow simulations. It is meant to provide a testbed to build reduced model for relevant challenging reacting flow problems using different methods. In addition, this collection also serves to engage a broad community of experts in computational science and the field of engineering to address certain challenges in constructing reduced models for reacting flow simulations. All the datasets in this collection were generated under the Air Force Center of Excellence on Multi-Fidelity Modeling of Rocket Combustion Dynamics and the goal of the center is to advance the state-of-the-art in Reduced Order Models (ROMs) and enable efficient and accurate prediction of instabilities in liquid fueled rocket combustion systems.
The outer epithelial layer of zebrafish retinae contains a crystalline array of cone photoreceptors, called the cone mosaic. As this mosaic grows by mitotic addition of new photoreceptors at the rim of the hemispheric retina, topological defects, called “Y-Junctions”, form to maintain approximately constant cell spacing. The generation of topological defects due to growth on a curved surface is a distinct feature of the cone mosaic not seen in other well-studied biological patterns like the R8 photoreceptor array in the _ Drosophila compound eye. Since defects can provide insight into cell-cell interactions responsible for pattern formation, here we characterize the arrangement of cones in individual Y-Junction cores (see Set of images for Figures 1 and 2 and 6 and Supplementary Figure 7) as well as the spatial distribution of Y-junctions across entire retinae (see Dataset for analyzing spatial distribution of Y-junctions in flat-mounted retinae). We find that for individual Y-junctions, the distribution of cones near the core corresponds closely to structures observed in physical crystals (see Set of images for Figures 1 and 2 and 6 and Supplementary Figure 7). In addition, Y-Junctions are organized into lines, called grain boundaries, from the retinal center to the periphery (see Dataset for analyzing spatial distribution of Y-junctions in flat-mounted retinae and Dataset for measuring tendency of Y-junctions to line up into grain boundaries during incorporation into retinae). In physical crystals, regardless of the initial distribution of defects, defects can coalesce into grain boundaries via the mobility of individual particles. By imaging in live fish, we demonstrate that grain boundaries in the cone mosaic instead appear during initial mosaic formation, without requiring defect motion (see Dataset for measuring tendency of Y-junctions to line up into grain boundaries during incorporation into retinae and Dataset for analyzing Y-junction motion in live fish retinae). Motivated by this observation, we show that a computational model of repulsive cell-cell interactions generates a mosaic with grain boundaries (see Code and example simulations of phase-field crystal model (for cone mosaic formation)). In contrast to paradigmatic models of fate specification in mostly motionless cell packings (see Code and accompanying input data for simulating lateral inhibition on motionless cell packing), this finding emphasizes the role of cell motion, guided by cell-cell interactions during differentiation, in forming biological crystals. Such a route to the formation of regular patterns may be especially valuable in situations, like growth on a curved surface, where the resulting long-ranged, elastic, effective interactions between defects can help to group them into grain boundaries.
This collection is comprised of a number of works that collectively represent the imaging studies and information necessary for dosimetric analysis of a patient treated with Lutathera. All works may be used as standalone datasets or in conjunction with the others in this collection depending on the analysis performed. Files are stored using the DICOM standard widely accepted for storage and transmission of medical images and related information. All patient private information has been anonymized using MIM commercial software (MIM Software Inc.).
Data from 2 patients, referred to as patient 4 and patient 6, has been provided in this collection and is divided among 6 works as outlined below:, 1) Pre-Therapy Diagnostic Images.
Description: Patient diagnostic scans performed prior to Lutathera treatment. Used for identifying lesions and measuring progression. Note that the date of the baseline scan may be several months before the Lutathera treatment and changes in the anatomy are possible.
Files: (1) Ga68 Dotatate PET/CT, Either: (1) MRI, (1) standalone diagnostic CT, 2) Planar Whole Body Scans.
Description: Planar whole body Lu-177 scans taken at 4 time points within a week after treatment. Two views (Anterior and Posterior) and 3 energy windows (one main window at 208 keV and 2 adjacent scatter windows) are available for each time point. The units of this image is counts. Energy window information, acquisition data/time and duration can be found in DICOM header.
Files: (6) individual images at each time point (24 total images per patient)
, 3) SPECT/CT Scans.
Description: Lu-177 SPECT/CT scans at 4 time points within a week after treatment (same time points as the planar scans). Images were acquired on a Siemens Intevo system and reconstructed using xSPECT Quant. The units of this image is Bq/mL. Information on the reconstruction, acquisition date/time, duration, Lu-177 administration time and activity can be found in the DICOM header.
Files: (1) Folder with reconstructed SPECT slices per time point (4 folders total per patient), (1) Folder containing co-registered CT slices per time point (4 folders total per patient), 4) Lesion and Organ Volumes of Interest.
Description: DICOM RT structure files containing organ and lesion volumes of interest (VOI) that were defined on the CT of the scan1 SPECT/CT in 3). Organs were defined using semi-automatic tools (atlas based and CNN-based) while lesions were defined manually by a radiologist guided by baseline scans. Only lesions >2 cc were defined.
Files: (1) File containing organ contours, (1) File containing lesion contours, 5) Time Integrated Activity Maps.
Description: A DICOM file containing the time-integrated activity map over all 4 time points within a week after treatment. This combines the SPECT/CT scans provided in 3) into a single integrated activity map. This map was generated via the MIM MRT Dosimetry package: The 4 time points were registered to the reference SPECT scan (time point 1) using a contour intensity based SPECT alignment and the voxel-level time-activity data was fit using exponential functions. Voxel-level integration was performed to generate the TIA map. The units of this image is Bq/mL * sec.
Files: (1) Folder with Time-integrated activity image per patient, and 6) Projection Data and CT based Attenuation Coefficient Maps.
Description: SPECT projection data for each of the 4 Lutathera scans taken within a week after treatment is provided in 3 forms: unaltered, Siemens [Reformatted], and Siemens [Advanced]. The difference between the Projections and the [Advanced] Projections is that the [Advanced] consists of uncorrected raw projection data and the other the corrected projection data (e.g. camera uniformity corrections). The [Advanced] projections are used in xSPECT reconstruction (where all corrections are done during the reconstruction), while the other is used in Flash 3D reconstruction. CT-based attenuation coefficient maps (mumaps) are provided for each of the 4 scans taken within a week after treatment. Two methods are provided for each mumap: xSPECT and F3D as the matrix size is different for the 2 cases (256 x 256 for xSPECT and 128 x 128 for Flash3D).
Files: (3) Folders containing raw SPECT projections, (2) Folders containing CT attenuation coefficient maps (mumaps)
This collection concerns the CRIMSON (CardiovasculaR Integrated Modelling and SimulatiON) software environment. CRIMSON provides a powerful, customizable and user-friendly system for performing three-dimensional and reduced-order computational haemodynamics studies via a pipeline which involves: 1) segmenting vascular structures from medical images; 2) constructing analytic arterial and venous geometric models; 3) performing finite element mesh generation; 4) designing, and 5) applying boundary conditions; 6) running incompressible Navier-Stokes simulations of blood flow with fluid-structure interaction capabilities; and 7) post-processing and visualizing the results, including velocity, pressure and wall shear stress fields. , The minimum specifications to run CRIMSON are: Any AMD64 CPU (note: Intel Core i series are AMD64), Windows (only tested on Windows 10 but might work on Windows 7), 8 GB of RAM
, If you are running non-trivial models you will want to have: Quad core CPU or higher, Solid state drive for storing data, Windows, 16 GB of RAM, Dedicated discrete GPU for rendering models.
, and Software in this collection is a snapshot; please visit https://github.com/carthurs/CRIMSONGUI & www.crimson.software for more general information and the most up to date version of the software.
CRIMSON: An Open-Source Software Framework for Cardiovascular Integrated Modelling and Simulation C.J. Arthurs, R. Khlebnikov, A. Melville, et al. bioRxiv 2020.10.14.339960; doi: https://doi.org/10.1101/2020.10.14.339960
Professor Revzen and his team at the Biologically Inspired Robotics and Dynamical Systems (BIRDS) Lab are working on discovering, modeling, and reproducing the strategies animals use when interacting with physical objects. This work consists of collaboration with biomechanists to analyze experimental data, developing new mathematical tools for modeling and estimation of model parameters, and construction of robots which employ the new principles.