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- Creator:
- Li, Jieming, Zhang, Leyou, Johnson-Buck, Alexander, and Walter, Nils G.
- Description:
- Traces from single-molecule fluorescence microscopy (SMFM) experiments exhibit photophysical artifacts that typically necessitate human expert screening, which is time-consuming and introduces potential for user-dependent expectation bias. Here, we have used deep learning to develop a rapid, automatic SMFM trace selector, termed AutoSiM, that improves the sensitivity and specificity of an assay for a DNA point mutation based on single-molecule recognition through equilibrium Poisson sampling (SiMREPS). The improved performance of AutoSiM is based on accepting both more true positives and fewer false positives than the conventional approach of hidden Markov modeling (HMM) followed by thresholding. As a second application, the selector was used for automated screening of single-molecule Förster resonance energy transfer (smFRET) data to identify high-quality traces for further analysis, and achieves ~90% concordance with manual selection while requiring less processing time. AutoSiM can be adapted readily to novel datasets, requiring only modest Transfer Learning.
- Keyword:
- deep learning, single-molecule fluorescence, total internal reflection microscopy, SiMREPS, smFRET, and Forster resonance energy transfer
- Citation to related publication:
- Li, J., Zhang, L., Johnson-Buck, A., & Walter, N. G. (2020). Automatic classification and segmentation of single-molecule fluorescence time traces with deep learning. Nature Communications, 11(1), 5833. https://doi.org/10.1038/s41467-020-19673-1 and Hayward, S., Lund, P., Kang, Q., Johnson-Buck, A., Tewari, M., Walter, N. (2018). Single-molecule microscopy image data and analysis files for "Ultra-specific and Amplification-free Quantification of Mutant DNA by Single-molecule Kinetic Fingerprinting" [Data set]. University of Michigan - Deep Blue. https://doi.org/10.7302/Z2CZ35DF
- Discipline:
- Science
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- Creator:
- Heath, Jeffrey
- Description:
- images of villages in Mali in which Bangime is the primary language. Each file name contains important information about the photos, and are structured thus: LanguageFamily_Language_IdentificationNumber_GeographicCoordinate_Description_Date_InitialsOfThePhotographer
- Keyword:
- Mali, Bangime, and villages
- Citation to related publication:
- Moran, Steven & Forkel, Robert & Heath, Jeffrey (eds.) 2016. Dogon and Bangime Linguistics. Jena: Max Planck Institute for the Science of Human History. http://dogonlanguages.org
- Discipline:
- Humanities
-
- Creator:
- Heath, Jeffrey
- Description:
- Images of villages in Mali in which Bankan Tey (Dogon family) is the primary language. Each file name contains important information about the photos, and are structured thus: LanguageFamily_Language_IdentificationNumber_GeographicCoordinate_Description_Date_InitialsOfThePhotographer
- Keyword:
- villages, Dogon, Bankan Tey, and Mali
- Citation to related publication:
- Moran, Steven & Forkel, Robert & Heath, Jeffrey (eds.) 2016. Dogon and Bangime Linguistics. Jena: Max Planck Institute for the Science of Human History. http://dogonlanguages.org
- Discipline:
- Humanities
-
- Creator:
- Curlis, JD, Renney, TJ, Davis Rabosky, AR, and Moore, TY
- Description:
- Efficient comparisons of biological color patterns are critical for understanding the mechanisms by which organisms evolve in ecosystems, including sexual selection, predator-prey interactions, and thermoregulation. However, elongate or spiral-shaped organisms do not conform to the standard orientation and photographic techniques required for automated analysis. Currently, large-scale color analysis of elongate animals requires time-consuming manual landmarking, which reduces their representation in coloration research despite their ecological importance. We present Batch-Mask: an automated and customizable workflow to facilitate the analysis of large photographic data sets of non-standard biological subjects. First, we present a user guide to run an open-source region-based convolutional neural network with fine-tuned weights for identifying and isolating a biological subject from a background (masking). Then, we demonstrate how to combine masking with existing manual visual analysis tools into a single streamlined, automated workflow for comparing color patterns across images. Batch-Mask was 60x faster than manual landmarking, produced masks that correctly identified 96% of all snake pixels, and produced pattern energy results that were not significantly different from the manually landmarked data set. The fine-tuned weights for the masking neural network, user guide, and automated workflow substantially decrease the amount of time and attention required to quantitatively analyze non-standard biological subjects. By using these tools, biologists will be able to compare color, pattern, and shape differences in large data sets that include significant morphological variation in elongate body forms. This advance will be especially valuable for comparative analyses of natural history collections, and through automation can greatly expand the scale of space, time, or taxonomic breadth across which color variation can be quantitatively examined.
- Keyword:
- convolutional neural network, photography, sensory ecology, color evolution, vision, and image segmentation
- Citation to related publication:
- Curlis, Renney, Davis Rabosky, Moore (submitted) Batch-Mask: An automated Mask R-CNN workflow to isolate non-standard biological specimens for color pattern analysis.
- Discipline:
- Engineering and Science
-
- Creator:
- Tye, Alexander R, Wolf, Aaron S, and Niemi, Nathan A
- Description:
- Detrital zircon age distributions provide robust insights into past sedimentary systems, but these age distributions are often complex and multi-peaked, with sample sizes too small to confidently resolve population distributions. This limited sampling hinders existing quantitative methods for comparing detrital zircon age distributions, which show systematic dependence on the sizes of compared samples. The proliferation of detrital zircon studies motivates the development of more robust quantitative methods. We present the first attempt, to our knowledge, to infer probability model ensembles (PMEs) for samples of detrital zircon ages using a Bayesian method. Our method infers the parent population age distribution from which a sample is drawn, using a Monte Carlo approach to aggregate a representative set of probability models that is consistent with the constraints that the sample data provide. Using the PMEs inferred from sample data, we develop a new estimate of correspondence between detrital zircon populations called Bayesian Population Correlation (BPC). Tests of BPC on synthetic and real detrital zircon age data show that it is nearly independent from sample size bias, unlike existing correspondence metrics. Robust BPC uncertainties can be readily estimated, enhancing interpretive value. When comparing two partially overlapping zircon age populations where the shared proportion of each population is independently varied, BPC results conform almost perfectly to expected values derived analytically from probability theory. This conformity of experimental and analytical results permits direct inference of the shared proportions of two detrital zircon age populations from BPC. We provide MATLAB scripts to facilitate the procedures we describe.
- Keyword:
- provenance, statistics, zircon, Bayesian, detrital, and density estimation
- Citation to related publication:
- A.R. Tye, A.S. Wolf, N.A. Niemi, Bayesian population correlation: A probabilistic approach to inferring and comparing population distributions for detrital zircon ages, Chemical Geology, Volume 518, 2019, Pages 67-78, ISSN 0009-2541, https://doi.org/10.1016/j.chemgeo.2019.03.039
- Discipline:
- Science
-
- Creator:
- Heath, Jeffrey
- Description:
- Five-part documentary on making apiaries and collecting honey. Vigué ethnicity, Viemo language. location: near Karangasso-Vigué, southwestern Burkina Faso. credits at end of videos. Other documentaries from Burkina may be added later.
- Keyword:
- Burkina Faso, Vigué, Viemo, beekeeping, and apiary
- Discipline:
- Humanities
-
- Creator:
- Reed, Matthew P., Boyle, K.
- Description:
- This is the first physical anthropomorphic test device to be based both on statistical body shape models as well as 3D printing.
- Keyword:
- Child Belt Fit Manikin
- Discipline:
- Engineering
-
- Creator:
- Heath, Jeffrey
- Description:
- Images of villages in Mali in which Ben Tey (Dogon family) is the primary language. Each file name contains important information about the photos, and are structured thus: LanguageFamily_Language_IdentificationNumber_GeographicCoordinate_Description_Date_InitialsOfThePhotographer
- Keyword:
- villages, Dogon, Ben Tey, and Mali
- Citation to related publication:
- Moran, Steven & Forkel, Robert & Heath, Jeffrey (eds.) 2016. Dogon and Bangime Linguistics. Jena: Max Planck Institute for the Science of Human History. http://dogonlanguages.org
- Discipline:
- Humanities
-
- Creator:
- BIRDS Lab, U. Michigan
- Description:
- These data were produced in an attempt to characterize the turning and steering behaviors of 1-DoF multi-legged (hexpedal in this case) robots. Such turning behaviors require sliding contact points. All the data is provided in a single, large .csv.gz file (416256 rows); additional details and example code in the README
- Keyword:
- robot, multilegged, and steering
- Citation to related publication:
- BIRDS Lab, U. BigAnt v6 robot motion tracking data - RAW dataset [Data set], University of Michigan - Deep Blue Data. https://doi.org/10.7302/024q-kk06, Revzen, S., & Guckenheimer, J. (2008). Estimating the phase of synchronized oscillators. Phys. Rev. E, 78, 051907. http://dx.doi.org/10.1103/PhysRevE.78.051907, and Dan Zhao and Shai Revzen 2020 Bioinspir. Biomim. 15 045001 https://doi.org/10.1088/1748-3190/ab84c0
- Discipline:
- Engineering
-
- Creator:
- BIRDS Lab, U. Michigan
- Description:
- These data were produced in an attempt to characterize the turning and steering behaviors of 1-DoF multi-legged (hexpedal in this case) robots. Such turning behaviors require sliding contact points. The .tar file contains multiple trials in .csv.gz format, with names following an informative naming convention documented in the README. Additional metadata for the trials is given in the metadata.py file in both machine and human readable form.
- Keyword:
- robot, multilegged, and steering
- Citation to related publication:
- Dan Zhao and Shai Revzen 2020 Bioinspir. Biomim. 15 045001 https://doi.org/10.1088/1748-3190/ab84c0
- Discipline:
- Engineering
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- Creator:
- BIRDS Lab, U. Michigan
- Description:
- These data were produced for ARO W911NF-14-1-0573 "Morphologically Modulated Dynamics" and ARO MURI W911NF-17-1-0306 "From Data-Driven Operator Theoretic Schemes to Prediction, Inference, and Control of Systems" to explore the trade-offs between various oscillator coupling models in modeling multilegged locomotion of Multipod robots with 6,8,10 and 12 legs. The data is stored in .csv.gz files, one file for each robot morphology. Details of how to run the processing code on the raw dataset to generate the processed files found here, as well as example code for loading the data found here, are in the README. This dataset is self contained and can be used on its own without running any of the provided code.
- Citation to related publication:
- Zhao, D. & Revzen, S. Multi-legged steering and slipping with low DoF hexapod robots Bioinspiration & biomimetics, 2020, 15, 045001 https://doi.org/10.1088/1748-3190/ab84c0, Zhao, D. Ph.D. Thesis "Locomotion of low-DOF multi-legged robots" University of Michigan 2021 https://deepblue.lib.umich.edu/handle/2027.42/169985, and BIRDS Lab Multipod robot motion tracking data - RAW data, doi:10.7302/m05a-0d90
- Discipline:
- Engineering and Science
-
- Creator:
- Sealey, Briana A., Larson, Joanna G., Westeen, Erin P., Sánchez-Paredes, Ciara M., Moore, Talia Y., and Davis Rabosky, Alison R.
- Description:
- In this study, we experimentally tested for the effects of four simulated predator cues on defensive displays in two species of South American calico snakes (genus Oxyrhopus). We found that juvenile snakes were both more likely to respond and to respond more strongly than adults and that displays were most common in response to tactile stimuli than to other treatments. However, we also found broad similarity across both simulated predator treatments and species in the components used in each snake’s defensive display, suggesting a high degree of stereotyping. This research suggests an important role for both ontogeny and intensity of predation risk in structuring variation in defensive behavior in Neotropical snakes and emphasizes the foundational importance of context dependence in conceptual frameworks for understanding predator-prey interactions. and *On January 4, 2024, “Supplementary_material.pdf” was replaced with an updated version that has slightly different versions of Figures S4 and S8 after an error in code was corrected. Within “HeatmapFigures.zip,” two code files, “IndividualHeatmaps_matrices.R” and “FigureS4_S8_averagedHeatmaps.R” were updated to correct the code error. Three additional files were added to both the “figures” and “matrices” folders within the subfolder “heatmaps.” These files represent the correlation matrices, by body part, and graphical representation of the matrices for one experimental trial that had previously been excluded due to the code error.
- Keyword:
- context-dependence, anti-predator behavior, Peruvian Amazon, ontogeny, coral snake mimicry, Colubridae
- Citation to related publication:
- Sealey, B.A.*, Larson, J.G.*, Westeen, E.P., Sanchez-Paredes, C.M., Moore, T.Y., Davis Rabosky, A.R. Body size and predator cues structure variation in defensive displays of Neotropical calico snakes (Oxyrhopus spp.). Ethology. In press. *Authors contributed equally
- Discipline:
- Science
-
Bounce-Averaged Quasi-Linear Diffusion Model Simulation Input/Output on Mars’ Crustal Magnetic Field
- Creator:
- Alexander Shane
- Description:
- To study the effect of whistler mode waves on the superthermal electron velocity space at Mars, a numerical model was built to solve the bounce-averaged quasi-linear diffusion equation on a crustal field. This dataset includes the input and output variables to this model for the simulations performed in Shane and Liemohn, 2022. The studies using this dataset were conducted by Alex Shane in the Climate and Space Sciences and Engineering Department at the University of Michigan. This research was supported by the National Aeronautics and Space Administration (NASA) Grant NNX16AQ04G to the University of Michigan and the Rackham Predoctoral Fellowship.
- Keyword:
- Mars, Electron, and Crustal Fields
- Citation to related publication:
- Shane, A. D., & Liemohn, M. W. (2022). Modeling wave-particle interactions with photoelectrons on the dayside crustal fields of Mars. Geophysical Research Letters, 49, e2021GL096941. https://doi.org/10.1029/2021GL096941
- Discipline:
- Science
-
- Creator:
- Heath, Jeffrey
- Description:
- Images of villages in Mali in which Bozo Jenama (Mande family) is the primary language. Each file name contains important information about the photos, and are structured thus: LanguageFamily_Language_IdentificationNumber_GeographicCoordinate_Description_Date_InitialsOfThePhotographer
- Keyword:
- villages, Mande, Bozo Jenama, and Mali
- Citation to related publication:
- Moran, Steven & Forkel, Robert & Heath, Jeffrey (eds.) 2016. Dogon and Bangime Linguistics. Jena: Max Planck Institute for the Science of Human History. http://dogonlanguages.org
- Discipline:
- Humanities
-
Bozo languages of Mali (spreadsheets, media)
User Collection- Creator:
- Heath, Jeffrey G.
- Description:
- This collection will archive lexical spreadsheets, audio files, geographic information, images, and videos that complement the reference grammars in pdf and docx form in the collection “Bozo languages of Mali (documents)” that are archived in Deep Blue Documents ( https://dx.doi.org/10.7302/6632). See the “readme” for that collection and the introductory material in the reference grammars for general information about the languages and the fieldwork., The initial material archived in the present collection consists of audio files. They are recordings of narratives, interviews, and conversations. Some of them have been transcribed and are presented as appendices in the reference grammars. Others have not been transcribed; they are presented here in the hope that they can eventually be transcribed or at least listened to by native speakers. If the author is able to transcribe some of them in the future, the transcriptions will be added here (and to the Deep Blue Documents collection)., Many of the recordings, as well as most of the images and videos to be added to this collection, have been made by project assistant Minkailou Djiguiba. He has courageously traveled into Bozo-speaking zones, some of which are highly insecure, to do this work. In addition, he has been instrumental in recruiting and transporting Bozo speakers to the author’s base in Bobo Dioulasso where much of the grammatical and lexical work has been done., and The author’s fieldwork is supported by grant PD-1941828 (2020-2024) from the National Science Foundation, Documenting Endangered Languages program, which is also supported by the National Endowment for the Humanities.
- Keyword:
- Bozo, Jenaama, Cliffs, Kelenga, Tigemaxo, and Tiéyaxo
- Discipline:
- Humanities
5Works -
- Creator:
- Heath, Jeffrey
- Description:
- Images of villages in Mali in which Bunoge (Dogon family) is the primary language. Each file name contains important information about the photos, and are structured thus: LanguageFamily_Language_IdentificationNumber_GeographicCoordinate_Description_Date_InitialsOfThePhotographer
- Keyword:
- villages, Dogon, Bunoge, and Mali
- Citation to related publication:
- Moran, Steven & Forkel, Robert & Heath, Jeffrey (eds.) 2016. Dogon and Bangime Linguistics. Jena: Max Planck Institute for the Science of Human History. http://dogonlanguages.org
- Discipline:
- Humanities
-
Burkina Faso documentary videos
User Collection- Creator:
- Heath, Jeffrey G.
- Description:
- This is a collection of mostly short documentary-style videos related to linguistic fieldwork in southwestern Burkina Faso. The initial batch consists of videos produced in 2023, and others will be added. Versions of some of these videos overdubbed in native languages will also be produced for local consumption in Burkina. Most of the videos document everyday practical activities; some also feature useful native plants and insects. This collection is parallel to collections of videos from neighboring Mali: see "Central Mali documentary videos" (with documentaries produced up to 2018) and "Mali documentary videos from 2023 on" for the newer ones. A small collection from north-central Côte d'Ivoire is also in the works. Within each collection, the videos are organized into "works" based on the general type of activity documented.
- Keyword:
- Burkina Faso and documentary videos
- Discipline:
- Humanities
7Works -
- Creator:
- Lynch, Erin and van der Pluijm, Ben
- Description:
- High-resolution, low-angle XRD analysis of oriented clay samples (.txt files) and TC/EA, Mass Spectronometric analysis of oxygen and hydrogen isotopes (.xslx files)
- Keyword:
- fault gouge and geofluids
- Citation to related publication:
- In submission
- Discipline:
- Science
-
- Creator:
- Forrest, Stephen R., Panda, Anurag, Qu, Yue, Che, Xiaozhou, Coburn, Caleb, and Burlingame, Quinn
- Description:
- Mathematica Diffusion Simulation: Programmed by Coburn, Caleb. Simulation of diffusion in organic heterostructures, including least square fits and statistical goodness of fit analysis. Used to calculate fits to transient data in Fig 1, 3 and Extended Data Fig.2. Example data file included for download Matlab Montecarlo simulation: Programmed by Coburn, Caleb. Montecarlo simulation of charge diffusion on a cubic lattice to determine lateral diffusion length as a function of barrier height, assuming thermionic emission over the barrier. Matlab 2D Diffusion Simulation:Programmed by Coburn, Caleb. Modified from BYU Physics 430 Course Manual. Simulates diffusion around a film discontinuity, such a cut. Used to generate fits to Extended Data Fig. 1
- Keyword:
- Organic semiconductors and Charge diffusion
- Citation to related publication:
- Burlingame, Q., Coburn, C., Che, X., Panda, A., Qu, Y., & Forrest, S. R. (2018). Centimetre-scale electron diffusion in photoactive organic heterostructures. Nature, 554(7690), 77-80. https://doi.org/10.1038/nature25148
- Discipline:
- Science
-
- Creator:
- Heath, Jeffrey
- Description:
- Videos produced in the course of linguistic fieldwork. Most are presented here in three different video formats. "Gardening Diondiori" illustrates dry-season farming mostly of cash crops using ground water (springs, drying ponds and rivers, underground water sources). The other videos in this block are of ordinary rainwater agriculture done in the rainy season, featuring Dogon people and, in the case of "Rice harvest and threshing," Bangande (speakers of Bangime), who have the same agricultural methods. The principal rainy season crop in the zone is millet (Cenchrus spicatus), but most of the documentaries here are about secondary crops (cowpea, fonio, groundnut, peanut, groundnut, roselle, rice, sesame, sweet potato). "Driving off grain-eating birds" is based on an unsteady cellphone video brought to us, except for the final segments which we shot.
- Keyword:
- Mali, Dogon, and agriculture
- Citation to related publication:
- Moran, Steven & Forkel, Robert & Heath, Jeffrey (eds.) 2016. Dogon and Bangime Linguistics. Jena: Max Planck Institute for the Science of Human History. http://dogonlanguages.org
- Discipline:
- Humanities