Craniosynostosis is the premature fusion of cranial bones. The goal of this study was to determine if delivery of recombinant tissue nonspecific alkaline phosphatase (TNAP) could prevent or diminish the severity of craniosynostosis in a C57BL/6 FGFR2C342Y/+ model of neonatal onset craniosynostosis or a BALB/c FGFR2C342Y/+ model of postnatal onset craniosynostosis. Mice were injected with a lentivirus encoding a mineral targeted form of TNAP immediately after birth. Cranial bone fusion as well as cranial bone volume, mineral content and density were assessed by micro computed tomography. Craniofacial shape was measured with calipers., Alkaline phosphatase, alanine amino transferase (ALT) and aspartate amino transferase (AST) activity levels were measured in serum. Neonatal delivery of TNAP diminished craniosynostosis severity from 94% suture obliteration in vehicle treated mice to 67% suture obliteration in treated mice, p<0.02) and the incidence of malocclusion from 82.4% to 34.7% (p<0.03), with no effect on cranial bone in C57BL/6 FGFR2C342Y/+ mice. In contrast, treatment with TNAP improved cranial bone volume (p< 0.01), density (p< 0.01) and mineral content (p< 0.01) but had no effect on craniosynostosis or malocclusion in BALB/c FGFR2C342Y/+ mice. , These results indicate that post-natal recombinant TNAP enzyme therapy diminishes craniosynostosis severity in the C57BL/6 FGFR2C342Y/+ neonatal onset mouse model of Crouzon syndrome, and that effects of exogenous TNAP are genetic background dependent., and Included in this collection is one set of images representing the C57BL/6 FGFR2C342Y/+ model of neonatal onset craniosynostosis, and one for the BALB/c FGFR2C342Y/+ model of postnatal onset craniosynostosis
In a broad sense, this project explores morphological and phonological processing in English monolinguals and two bilingual populations, Chinese-English and Spanish-English, using a battery of standardized and self-developed behavioral measures, as well as fNIRS neuroimaging. (T1=NEW PARTICIPANTES -TESTED BEHAVIORAL AND fNIRS-, T2= RETURNING PARTICIPANTS -JUST TESTED WITH BEHAVIORAL ASSESSMENTS)
Appendix1: Differential expression data for zebrafish regeneration and mouse degeneration models.
Appendix2: Gene ontology data for zebrafish regeneration and mouse degeneration models.
Appendix3: Pathway data for zebrafish regeneration and mouse degeneration models.
Appendix4: Differential expression data and genes within linked peaks for mi2004 mutants.
Appendix5: Gene ontology data for mi2004 mutants.
Appendix6: Pathway data for mi2004 mutants.
Appendix7: Linkage plots for mi2004 mutants.
Appendix8: Inverse PCR and genome-walking data.
Sifuentes, C. J. (2016). Regulation of Müller glial stem cell properties: Insights from a zebrafish model (Doctoral dissertation). Retrieved from http://hdl.handle.net/2027.42/135939
This collection was produced as part of the project, “A ‘Big Data’ Approach to Understanding Neighborhood Effects in Chronic Illness Disparities.” The Investigators for the project are Tiffany Veinot, Veronica Berrocal, Phillipa Clarke, Robert Goodspeed, Daniel Romero, and VG Vinod Vydiswaran from the University of Michigan. The study took place from 2015-2016, with funding from the University of Michigan’s Social Sciences Annual Institute, MCubed, and the Sloan and Moore Foundations.
Contact: Tiffany Veinot, MLS, PhD
Office: 3443 North Quad
Phone: 734/615-8281
Email: tveinot@umich.edu
MCubed project page:
https://mcubed.umich.edu/projects/%E2%80%9Cbig-data%E2%80%9D-approach-understanding-neighborhood-effects-chronic-illness-disparities
Fossil energy production, processing, flaring, and transmission all can harm climate and air quality by emitting greenhouse gases and air pollutants. Studies now show that onshore oil and gas production emit much more methane than what is inventoried, and that local air quality impacts can be significant, however, natural gas flaring and offshore systems have been largely overlooked.
The F3UEL (Flaring & Fossil Fuels: Uncovering Emissions & Losses) project aims to address these gaps by improving our understanding of offshore emissions, characterizing how flares behave in the real world, identifying what portion of the offshore system is responsible for emissions, and determining how such systems can be monitored.
Spanning three years (2020-2022), the project employed an aircraft platform to measure including both greenhouse gas and air quality measurements. To sample the largest regions of current and potential future offshore production and flaring, airborne measurements targeted the Gulf of Mexico, offshore California and Alaska, the Bakken Formation (North Dakota) and the Permian and Eagle Ford Basins (Texas).
Data provided here includes the airborne measurements collected using Scientific Aviation’s Mooney aircraft platform, equipped with spectroscopic instrumentation to measure methane, carbon dioxide, water vapor, nitrous oxide, and nitrogen oxide, in addition to meteorological variables such as wind speed and direction. Data products from our analysis of these airborne measurements are also provided, including estimated flare destruction removal efficiency for the Bakken, Eagle Ford, and Permian basins.
Each data file is in .csv format and is accompanied by a readme file with further information and descriptors of the variables included. All users should cite the papers and datasets provided in the readme files for each individual dataset.
Website: https://graham.umich.edu/f3uel
This project is funded by the Alfred P. Sloan Foundation with additional support from the Environmental Defense Fund, Scientific Aviation, and University of Michigan (College of Engineering, Climate and Space Sciences and Engineering; Graham Sustainability Institute).