Targeted metabolomics analysis of primary WT murine bone marrow-derived macrophages (BMDM) mock-treated or infected with methicillin-resistant Staphylococcus aureus (MRSA) (File S1). Targeted metabolomics analysis of mock-treated or MRSA-infected WT or Ifnar1-/- iBMDM (File S2).
Type I interferon governs immunometabolic checkpoints that coordinate inflammation during Staphylococcal infection Mack B. Reynolds, Benjamin Klein, Michael J. McFadden, Norah K. Judge, Hannah E. Navarrete, Britton C Michmerhuizen, Dominik Awad, Tracey L. Schultz, Paul W. Harms, Li Zhang, Teresa R. O’Meara, Jonathan Z. Sexton, Costas A. Lyssiotis, J. Michelle Kahlenberg, Mary X. O’Riordan bioRxiv 2024.01.10.575104; doi: https://doi.org/10.1101/2024.01.10.575104 and Reynolds et al., Type I Interferon governs immunometabolic checkpoints that coordinate inflammation during Staphylococcal infection, Cell Reports (2024), https://doi.org/10.1016/j.celrep.2024.114607
Untargeted lipidomics (Data S1) and targeted metabolomics (Data S2) analysis from in vitro culture of a murine macrophage cell line expressing shRNA targeted to Cardiolipin synthase (CRLS1), referred to as CRLS1 knockdown (KD), or a paired non-target shRNA-expressing (NT-Control). CRLS1 KD and NT-Control macrophages were either directly analyzed (untargeted lipidomics) or stimulated with lipopolysaccharide for a variety of timepoints and then analyzed (targeted metabolomics). Datasets are available as .csv files.
Reynolds M.B. et al. (2023). Cardiolipin coordinates inflammatory metabolic reprogramming through regulation of Complex II disassembly and degradation. Science Advances, 9(5). DOI: 10.1126/sciadv.ade8701
