Work Description

Date: 30 June 2022 Dataset Title: Trimester-specific phthalate exposures in pregnancy are associated with circulating metabolites in children Dataset Creators: Goodrich J.M., Tang L.,Rodr�guez-Carmona Y., Meijer J.L, Perng W., Watkins D.J., Meeker J.D., Mercado-Garc�a A., Cantoral A., Song P.X., T�llez-Rojo M.M., Peterson K.E. Dataset Contact: Jaclyn M. Goodrich (gaydojac@umich.edu) Funding: NIEHS P20 ES018171; US EPA RD834800 Key Points: -Phthalates are endocrine-disrupting chemicals found in many products that are linked to adverse health effects in children and adults. -We measured phthalate exposures during each trimester of pregnancy and followed-up the offspring later in childhood. -We measured children�s metabolites via untargeted metabolomics. -Prenatal phthalate exposures were associated with altered metabolites in childhood. Research Overview: Phthalates are chemicals found in many products that humans are exposed to. Prenatal exposure to phthalates has been associated with adverse outcomes that are detected in childhood, adolescence, and even adulthood. In this study, we sought to identify subtle biological changes in the metabolome of children that were exposed to phthalates during gestation. We hypothesized that prenatal phthalate exposures would alter metabolic pathways related to adiposity and cardiometabolic health. The article is under review (citation to be added when paper is published). The data included here encompass all exposure, demographic, and untargeted metabolomics data needed for the analysis described in the manuscript. Methodology: We collected samples and data from mother-child dyads from the Early Life Exposure in Mexico to ENvironmental Toxicants (ELEMENT) birth cohort during pregnancy and at a follow-up visit when children were 8-14 years of age. We measured 9 phthalate metabolites and bisphenol A in maternal spot urine samples obtained during each trimester of pregnancy via ICP-MS. Urinary phthalate concentrations were corrected for urinary specific gravity and natural log-transformed. The offspring (110 boys and 124 girls) were followed-up in childhood. We collected information on anthropometry and demographics. Serum samples were analyzed for relative abundance of metabolites via an untargeted metabolomics approach. collected samples for untargeted metabolomics platform. Metabolic features detected in at least >70% of samples with identities that could be annotated were selected for analysis and are included in the dataset (n=572 metabolites). Peak intensities were adjusted for batch and missing values were imputed followed by log transformation and standardization (to mean 0 variance 1). Multivariable linear regression models were then used to assess the associations between individual metabolites and individual trimester exposures, adjusting for children�s age, BMI z-score, sex and pubertal onset. Instrument and/or Software specifications: NA Files contained here: The data presented here focuses on prenatal phthalate exposures, demographics (at birth and at follow-up in childhood), and metabolomics (in childhood). Data is from a cohort based in Mexico City. Survey data and biological samples were collected during in-person study visits (1997-2014). The data files are described below: - Phthalate Metabolites and Demographic Covariates_Dictionary.docx --> Data dictionary for exposure variables and Demographic Covariates - Phthalate Metabolites and Demographic Covariates_Dataset.csv --> Final dataset for exposure variables and Demographic Covariates in �.csv� format - StandarizedMetabolites572.csv --> Final dataset for untargeted metabolomics used in the manuscript (following standardization) in �.csv� format - MetabolitesInfo572.csv --> Data dictionary providing details on each metabolite within the StandardizedMetabolites572 dataset in �.csv� format - MRC2 Untargeted Metabolomics Report.docx --> Document from the laboratory performing metabolomics analysis providing detail on the analytical procedure and quality control. - Informed Consent ELEMENT_P20_EN.pdf--> Original informed consent in English used during the data collection visit - Informed Consent ELEMENT_P20_SP.pdf--> Original informed consent in Spanish used during the data collection visit - Informed Assent ELEMENT_P20_EN.pdf--> Original informed assent for minors in English used during the data collection visit - Informed Assent ELEMENT_P20_SP.pdf--> Original informed assent for minors in Spanish used during the data collection visit Related publication(s): Goodrich J.M., Tang L.,Rodr�guez-Carmona Y., Meijer J.L, Perng W., Watkins D.J., Meeker J.D., Mercado-Garc�a A., Cantoral A., Song P.X., T�llez-Rojo M.M., Peterson K.E. Trimester-specific phthalate exposures in pregnancy are associated with circulating metabolites in children. PLoS One. (Under revision � forthcoming.) Use and Access: This data set is made available under a Creative Commons Attribution-Noncommercial 4.0 International (CC BY-NC 4.0) To Cite Data: Goodrich J.M., Tang L.,Rodr�guez-Carmona Y., Meijer J.L, Perng W., Watkins D.J., Meeker J.D., Mercado-Garc�a A., Cantoral A., Song P.X., T�llez-Rojo M.M., Peterson K.E. (2022). Trimester-specific phthalate exposures in pregnancy are associated with circulating metabolites in children [Covariate Data set]. University of Michigan - Deep Blue.