Now showing items 371-380 of 444
Cartridge Methods for Oligonucleotide Purification
(Wiley Periodicals, Inc., 2000-07)
Protocols are given for purification of oligonucleotides by dimethoxytrityl‐sensitive and affinity desalting methods. The protocols are applicable for many of the convenient disposable products available for rapid ...
Micropatterned Fiber Scaffolds for Spatially Controlled Cell Adhesion
(WILEY-VCH Verlag, 2009-10-01)
Because the local microstructure plays a pivotal role for many biological functions, a wide range of methods have been developed to design precisely engineered substrates for both fundamental biological studies and ...
A proteomic analysis of Psychrobacter articus 273-4 adaptation to low temperature and salinity using a 2-D liquid mapping approach
(WILEY-VCH Verlag, 2007-02)
Psychrobacter 273-4 was isolated from a 20 000–40 000–year-old Siberian permafrost core, which is characterized by low temperature, low water activity, and high salinity. To explore how 273-4 survives in the permafrost ...
Chemistry of CpG DNA
(Wiley Periodicals, Inc., 2003-03)
The vertebrate immune system can recognize specific pathogen‐associated molecular patterns in invading microorganisms, including the unmethylated CpG dinucleotide. This unit discusses the receptors that recognize CpG motifs ...
Incorporation of Halogenoalkyl, 2‐Pyridyldithioalkyl, or Isothiocyanate Linkers into Ligands
(IRL PressWiley Periodicals, Inc., 2001-07)
Ligands can be introduced at the 5’ terminus of an oligonucleotide by adding a linker to the ligand and modifying the 5’ terminus of the oligonucleotide. These are then reacted to give the ligand‐oligonucleotide conjugate. ...
Phase II trial of fenretinide in advanced renal carcinoma
(Kluwer Academic Publishers; Springer Science + Business Media, Inc., 2005-01)
Purpose : Fenretinide, a synthetic form of retinoid, induced apoptosis even in chemotherapy resistant cell lines. A phase II study was hence conducted to evaluate toxicity and efficacy of fenretinide in metastatic renal ...
Raulin award lecture: Wilson's disease therapy with zinc and tetrathiomolybdate
(John Wiley & Sons, Inc., 2000)
Zinc and tetrathiomolybdate, effective, nontoxic drugs, have now been added to the treatment of most phases of Wilson's disease. Zinc is used for maintenance as well as treating presymptomatic, pregnant, and pediatric ...
Impact of an extruded nucleotide on cleavage activity and dynamic catalytic core conformation of the hepatitis delta virus ribozyme
(Wiley Subscription Services, Inc., A Wiley Company, 2007-04-05)
The self-cleaving hepatitis delta virus (HDV) ribozyme is essential for the replication of HDV, a liver disease causing pathogen in humans. The catalytically critical nucleotide C75 of the ribozyme is buttressed by a trefoil ...
Statistical mechanics of protein folding by cluster distance geometry
(Wiley Subscription Services, Inc., A Wiley Company, 2004-10-15)
This is our second type of model for protein folding where the configurational parameters and the effective potential energy function are chosen in such a way that all conformations are described and the canonical partition ...
Existence of a critical carbon number in the aging of a wax-oil gel
(Wiley Subscription Services, Inc., A Wiley Company, 2001-09)
A fundamental study was carried out to understand the aging (or hardening) of the wax-oil gels formed in the subsea pipelines during the flow of crude oil from offshore wells to shore. The aging process is a counterdiffusion ...