Now showing items 831-840 of 897
Mechanism and Specificity of Human DNA Ligase I
(2019)
Cellular DNA replication and repair pathways conclude with faithful rejoining of broken phosphodiester bonds by DNA ligases. Therefore, a thorough dissection of the molecular underpinnings of DNA ligation is crucial to a ...
Mapping Protein-Protein Interactions in Self-Sufficient Cytochrome P450 Enzymes
(2021)
Cytochrome P450 (P450) proteins are heme-containing mixed function oxidase enzymes that play a critical role in the metabolism of drugs and toxicants. For P450 enzyme catalysis, a flavin-containing reductase must shuttle ...
Structural and Biochemical Insights into Methylation Site and State Specificity of JMJD2 Lysine Demethylases.
(2012)
The human JMJD2/KDM4 family of histone lysine demethylases comprises four homologs: JMJD2A, JMJD2B, JMJD2C and JMJD2D. These enzymes have been implicated in a number of biological processes such as transcriptional activation, ...
Regulation of Gene Expression Through Nucleic Acid Binding Proteins: New Paradigms, Perspectives, and Tools
(2019)
More than 50 years ago, Jacob and Monod first laid out a model for the regulation of gene expression through the interaction of proteins and nucleic acids. With the advent of high-throughput sequencing and the unprecedented ...
Development of a Chemoproteomic Approach to Study Kinase-Substrate Interactions and the Discovery of CDK4 as a 4E-BP1 Kinase
(2019)
An instrumental aspect of cancer progression is the acquired deregulation of the cellular processes that regulate growth and proliferation. Most of these processes are, at some level, controlled by a kinase-catalyzed change ...
Inhibiting Functional Amyloid Formation Using Protein Engineering
(2022)
Amyloids are a class of protein assembly known for their extreme stability and ordered, fibrous structure. Functional amyloids are a subclass of amyloids that can be found in all domains of cellular life. Functional amyloids ...
Architecture-Function Analysis of the Kinetochore Reveals the Mechanism of Spindle Assembly Checkpoint Signaling.
(2015)
In a dividing cell, equal segregation of chromosomes between the two daughters is necessary for accurate genome inheritance. For successful segregation, chromosomes must attach to and move along microtubule tracks provided ...
Intracellular Trafficking Regulators for the Atherogenic Lipoproteins LDL and Lp(a)
(2022)
Elevated levels of low-density lipoprotein (LDL) and its derivative lipoprotein(a) (Lp(a)) in the bloodstream are directly implicated in the development and progression of atherosclerotic cardiovascular diseases (ASCVD). ...
Examination of Aggregation Prone Proteins and their Higher Order Interactions by Ion Mobility-Mass Spectrometry.
(2015)
Amyloid forming peptides and proteins present an extreme challenge for modern analytical measurement techniques. When natively-folded biomolecules partition into misfolded forms, they produce a myriad of inherently unstable ...
Combining Active Site and Allosteric Inhibitors to Study Protein Tyrosine Kinases
(2016)
Kinases play a crucial role in a variety of cell signaling pathways that control processes such as: growth, motility, and angiogenesis. Not surprisingly, dysregulation of proper kinase signaling often leads to cancer. Since ...