Heterologous expression of tylosin polyketide synthase and production of a hybrid bioactive macrolide in Streptomyces venezuelae
dc.contributor.author | Jung, Won Seok | en_US |
dc.contributor.author | Hong, Jay Sung Joong | en_US |
dc.contributor.author | Lee, Sang Kil | en_US |
dc.contributor.author | Sohng, Jae Kyung | en_US |
dc.contributor.author | Park, Sung Ryeol | en_US |
dc.contributor.author | Kim, Byung Gee | en_US |
dc.contributor.author | Choi, Cha Yong | en_US |
dc.contributor.author | Jeong, Soon Jeong | en_US |
dc.contributor.author | Han, Ah Reum | en_US |
dc.contributor.author | Sherman, David H. | en_US |
dc.contributor.author | Yoon, Yeo Joon | en_US |
dc.date.accessioned | 2006-09-11T16:33:25Z | |
dc.date.available | 2006-09-11T16:33:25Z | |
dc.date.issued | 2006-02-22 | en_US |
dc.identifier.citation | Jung, Won Seok; Lee, Sang Kil; Hong, Jay Sung Joong; Park, Sung Ryeol; Jeong, Soon Jeong; Han, Ah Reum; Sohng, Jae Kyung; Kim, Byung Gee; Choi, Cha Yong; Sherman, David H.; Yoon, Yeo Joon; (2006). "Heterologous expression of tylosin polyketide synthase and production of a hybrid bioactive macrolide in Streptomyces venezuelae ." Applied Microbiology and Biotechnology (): 1-7. <http://hdl.handle.net/2027.42/45861> | en_US |
dc.identifier.issn | 0175-7598 | en_US |
dc.identifier.issn | 1432-0614 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/45861 | |
dc.description.abstract | Tylosin polyketide synthase (Tyl PKS) was heterologously expressed in an engineered strain of Streptomyces venezuelae bearing a deletion of pikromycin PKS gene cluster using two compatible low-copy plasmids, each under the control of a pikAI promoter. The mutant strain produced 0.5 mg/l of the 16-membered ring macrolactone, tylactone, after a 4-day culture, which is a considerably reduced culture period to reach the maximum production level compared to other Streptomyces hosts. To improve the production level of tylactone, several precursors for ethylmalonyl-CoA were fed to the growing medium, leading to a 2.8-fold improvement (1.4 mg/ml); however, switching the pikAI promoter to an actI promoter had no observable effect. In addition, a small amount of desosamine-glycosylated tylactone was detected from the extract of the mutant strain, revealing that the native glycosyltransferase DesVII displayed relaxed substrate specificity in accepting the 16-membered ring macrolactone to produce the glycosylated tylactone. These results demonstrate a successful attempt for a heterologous expression of Tyl PKS in S. venezuelae and introduce S. venezuelae as a rapid heterologous expression system for the production of secondary metabolites. | en_US |
dc.format.extent | 187102 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Springer-Verlag | en_US |
dc.title | Heterologous expression of tylosin polyketide synthase and production of a hybrid bioactive macrolide in Streptomyces venezuelae | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Life Sciences Institute, Department of Medicinal Chemistry, University of Michigan, 210 Washtenaw Avenue, Ann Arbor, MI, 48109-2216, USA, | en_US |
dc.contributor.affiliationother | Division of Nano Sciences, Department of Chemistry, Ewha Woman’s University, 11-1 Daehyun-dong, Seodaemun-gu, Seoul, 120-750, South Korea, | en_US |
dc.contributor.affiliationother | Interdisciplinary Program of Biochemical Engineering and Biotechnology, Seoul National University, San 56-1, Shilim-dong, Gwanak-gu, Seoul, 151-742, South Korea, | en_US |
dc.contributor.affiliationother | School of Chemical and Biological Engineering, Seoul National University, San 56-1, Shilim-dong, Gwanak-gu, Seoul, 151-742, South Korea, | en_US |
dc.contributor.affiliationother | Interdisciplinary Program of Biochemical Engineering and Biotechnology, Seoul National University, San 56-1, Shilim-dong, Gwanak-gu, Seoul, 151-742, South Korea, | en_US |
dc.contributor.affiliationother | Division of Nano Sciences, Department of Chemistry, Ewha Woman’s University, 11-1 Daehyun-dong, Seodaemun-gu, Seoul, 120-750, South Korea, | en_US |
dc.contributor.affiliationother | Division of Nano Sciences, Department of Chemistry, Ewha Woman’s University, 11-1 Daehyun-dong, Seodaemun-gu, Seoul, 120-750, South Korea, | en_US |
dc.contributor.affiliationother | Interdisciplinary Program of Biochemical Engineering and Biotechnology, Seoul National University, San 56-1, Shilim-dong, Gwanak-gu, Seoul, 151-742, South Korea, | en_US |
dc.contributor.affiliationother | Institute of Biomolecule Reconstruction, Department of Pharmaceutical Engineering, Sun Moon University, Kalsan-ri, Tangjeong-myeon, Asan-si, Chungnam, 336-708, South Korea, | en_US |
dc.contributor.affiliationother | Interdisciplinary Program of Biochemical Engineering and Biotechnology, Seoul National University, San 56-1, Shilim-dong, Gwanak-gu, Seoul, 151-742, South Korea, ; School of Chemical and Biological Engineering, Seoul National University, San 56-1, Shilim-dong, Gwanak-gu, Seoul, 151-742, South Korea, | en_US |
dc.contributor.affiliationother | Interdisciplinary Program of Biochemical Engineering and Biotechnology, Seoul National University, San 56-1, Shilim-dong, Gwanak-gu, Seoul, 151-742, South Korea, ; School of Chemical and Biological Engineering, Seoul National University, San 56-1, Shilim-dong, Gwanak-gu, Seoul, 151-742, South Korea, | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/45861/1/253_2006_Article_318.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/s00253-006-0318-5 | en_US |
dc.identifier.source | Applied Microbiology and Biotechnology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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