Now showing items 41-50 of 70
Modulation of methylenetetrahydrofolate reductase activity by S-adenosylmethionine and by dihydrofolate and its polyglutamate analogues
(Elsevier, 1982)
Methylenetetrahydrofolate reductase catalyzes the reduction of methylenetetrahydrofolate ot methyltetrahydrofolate. This reaction commits one carbon units to the pathways of adenosylmethionine-dependent methylation in ...
Carbohydrate binding studies on the lectin from Datura stramonium seeds
(Elsevier, 1984-06)
The carbohydrate-binding properties of the Datura stramonium seed lectin were studied by equilibrium dialysis, quantitative precipitation of natural and synthetic glycoproteins, and hapten inhibition of precipitation. The ...
Characterization of the extended carbohydrate binding site of concanavalin A: Specificity for interaction with the nonreducing termini of [alpha]-(1-->2)-linked disaccharides
(Elsevier, 1981-07)
p-Nitrophenyl 2-O-[alpha]--galactopyranosyl-[alpha]--mannopyranoside and p-nitrophenyl 2-O-[alpha]--glucopyranosyl-[alpha]--mannopyranoside were synthesized and the interactions of these disaccharides with concanavalin A ...
A rapid method for the isolation of peroxisomes from rat liver
(Elsevier, 1986-11-15)
A preparative method for the isolation of peroxisomes from the liver of normal, untreated rats is described. The peroxisome-enriched "light mitochondrial" fraction is layered on a 30% Nycodenz (5-[N-2,3-dihydroxypropylac ...
Lectin-binding domains on laminin
(Elsevier, 1983-11)
The nature and location of carbohydrate moieties on the laminin molecule were identified by studying the binding affinity of a series of lectins for purified, proteasederived fragments of laminin. Laminin is a cross-shaped ...
Synthesis of N-acetyllactosamine containing a -[6-3H]galactopyranosyl group
(Elsevier, 1984-07-01)
A simple and convenient method for the introduction of radiolabel onto C-6' of N-acetyllactosamine is described. 1-N-Benzyl-3-O-[beta]--galactopyranosyl--arabinosylamine (1) was synthesized from 3-O-[beta]--galactopyrano ...
Carbohydrate binding properties of the stinging nettle (Urtica dioica) rhizome lectin
(Elsevier, 1986-08-15)
The interaction of the stinging nettle rhizome lectin (UDA) with carbohydrates was studied by using the techniques of quantitative precipitation, hapten inhibition, equilibrium dialysis, and uv difference spectroscopy. The ...
Identification of P-450ALC in microsomes from alcohol dehydrogenase-deficient deermice: Contribution to ethanol elimination in vivo
(Elsevier, 1988-07)
Isozyme 3a of rabbit hepatic cytochrome P-450, also termed P-450ALC, was previously isolated and characterized and was shown to be induced 3- to 5-fold by exposure to ethanol. In the present study, antibody against rabbit ...
N,N'-diacetylchitobionic N-alkylamides: Synthesis, and interaction with two 2-acetamido-2-deoxy--glucose-binding lectins
(Elsevier, 1981-10-16)
Condensation of N,N'-diacetylchitobiono-1,5-lactone, obtained by oxidation of N,N'-diacetylchitobiose, with n-alkylamines gave N,N'-diacetylchitobionic N-alkylamides. The interaction of these glycolipids with wheat-germ ...
Resolution of nucleotide sugars and oligosaccharides by lectin affinity chromatography
(Elsevier, 1980-02)
Bandeiraea simplicifolia I (BS I) isolectins, immobilized on Sepharose beads, specifically retarded low molecular weight ligands containing terminal [alpha]--galactopyranosyl and 2-acetamido-2-deoxy-[alpha]--galactopyranosyl ...